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A ThT Derivative as Zn2+ Sensor Based on DNA G-quadruplex

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Abstract

In this report, we developed a sensing strategy based on ThT-E (a ThT derivative) and DNA G-quadruplex for the label-free detection of Zn2+. In the absence of Zn2+, there was a fluorescence enhancement of ThT-E by interaction with human telomere sequence. On the addition of Zn2+, Zn2+ induced a more compact antiparallel G-quadruplex to release ThT-E, resulting in fluorescence quenching. The detection limit was 0.6996 μM, and the fluorescence intensity showed a good linear relationship with the concentration of Zn2+ in the range of 0-10 μM. This sensing strategy which only needs to mix two kinds of materials has the characteristics of label-feel, simple operation, short response time, economical and efficient.

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Availability of Data and Materials

The datasets used or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant Number 21807034, 82270303 ) and Natural Science Foundation of Hebei Province (Grant Number B2020209079).

Funding

This work was supported by the National Natural Science Foundation of China (Grant Number 21807034, 82270303) and Natural Science Foundation of Hebei Province (Grant Number B2020209079).

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Authors and Affiliations

  1. College of Chemical Engineering, North China University of Science and Technology, Tangshan, 063210, China

    **nyu Yuan, **ufeng Zhang, **shan Hu, Buyue Zhang, **aoying Ma, **xing He & Lei Shi

  2. Hebei Key Laboratory of Medical-Industrial Integration Precision Medicine, North China University of Science and Technology, Tangshan, 063210, China

    **ufeng Zhang & Lei Shi

Authors
  1. **nyu Yuan
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  2. **ufeng Zhang
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  4. Buyue Zhang
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  5. **aoying Ma
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  6. **xing He
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  7. Lei Shi
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Contributions

**nyu Yuan, **ufeng Zhang, Lei Shi contributed to the conception of the study; **nyu Yuan, Buyue Zhang, ****ng He, **aoying Ma performed the experiment; **nyu Yuan, **ufeng Zhang, Lei Shi, **shan Hu contributed significantly to analysis and manuscript preparation; **nyu Yuan, **ufeng Zhang, Lei Shi performed the data analyses and wrote the manuscript. All authors reviewed the manuscript.

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Correspondence to **ufeng Zhang.

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Yuan, X., Zhang, X., Hu, J. et al. A ThT Derivative as Zn2+ Sensor Based on DNA G-quadruplex. J Fluoresc 34, 353–358 (2024). https://doi.org/10.1007/s10895-023-03278-0

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