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Anti-angiogenic effect of hexahydrocurcumin in rat corneal neovascularization

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Abstract

Aim

This study was to investigate the anti-angiogenic effect of hexahydrocurcumin (HHC) to evaluate gene (p-basic fibroblast growth factor (bFGF)-SAINT-18 & p-vascular endothelial growth factor (VEGF)-SAINT-18 complex)-induced corneal neovascularization (CorNV) in rats.

Methods

CorNV was induced in 24 eyes of 24 rats. Four groups (Group A: 0 μg, B: 0.01 μg, C: 0.1 μg, and D: 1 μg) of HHC were prepared and implanted into the rat subconjunctival substantia propria 1.5 mm from the limbus at temporal side. The 1 μg of p-bFGF-SAINT-18 & p-VEGF-SAINT-18 complex were prepared and implanted into the rat corneal stroma 1.5 mm from the limbus at the same side. Inhibition of CorNV was observed and quantified from day 1 to day 60. bFGF and VEGF protein expression were analyzed by biomicroscopic examination, western blot analysis, and immunohistochemistry.

Results

Subconjunctival injection by 1 μg HHC successfully inhibited gene-induced CorNV in rats. bFGF and VEGF protein expression were reduced after 6 days. Meanwhile, the reduction of HLA-DR expression was detected.

Conclusions

Our study showed that the HHC might provide an important anti-angiogenesis factor to inhibit CorNV development at the corneal experimental angiogenesis model.

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Acknowledgements

This work was supported by a project grant from Chang Gung Memorial Hospital Grand (CMRPG6D0141).

Author information

Author notes

  1. Chien-Neng Kuo and Ching-Hsein Chen have contributed equally to this work.

Authors and Affiliations

  1. Department of Ophthalmology, Chang Gung Memorial Hospital, No. 6, W. Sec., Jiapu Rd, Puzi City, Chiayi County, 61363, Taiwan

    Chien-Neng Kuo, Jou-Cheng Huang, Li-Ju Lai & Chien-Hsiung Lai

  2. Chang Gung University College of Medicine, No. 259, Wenhua 1st Rd., Guishan Township, Taoyuan County, 33302, Taiwan

    Chien-Neng Kuo, Jou-Cheng Huang, Li-Ju Lai, Chien-Hsiung Lai & Chia-Hui Hung

  3. Chang Gung University of Science and Technology, No. 2, W. Sec., Jiapu Rd., Puzi City, Chiayi County, 61363, Taiwan

    Chien-Neng Kuo, Jou-Cheng Huang, Li-Ju Lai, Chien-Hsiung Lai & Chia-Hui Hung

  4. Department of Ophthalmology, Changhua Christian Hospital, Yun Lin Branch, No. 375, Shichang S. Rd., **luo Township, Yunlin County, 64866, Taiwan

    Chien-Neng Kuo

  5. Department of Microbiology, Immunology and Biopharmaceuticals, College of Life Sciences, National Chiayi University, No. 300 Syuefu Rd., Chiayi City, 60004, Taiwan

    Ching-Hsein Chen

  6. Department of Ophthalmology, Changhua Christian Hospital, No. 135, Nanxiao St., Changhua City, Changhua County, 50006, Taiwan

    San-Ni Chen

  7. School of Medicine, Chung-Shan Medical University, No. 110, Sec. 1, Jianguo N. Rd., Taichung City, 40201, Taiwan

    San-Ni Chen

  8. Department of Dermatology, Chang Gung Memorial Hospital, No. 6, W. Sec., Jiapu Rd., Puzi City, Chiayi County, 61363, Taiwan

    Chia-Hui Hung

  9. Department of Pharmacology, Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung City, 80708, Taiwan

    Chien-Hsing Lee

  10. School of Medical and Health Sciences, Fooyin University, No. 151, **xue Rd., Daliao Dist., Kaohsiung City, 83102, Taiwan

    Chung-Yi Chen

Authors
  1. Chien-Neng Kuo
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  2. Ching-Hsein Chen
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  6. Chien-Hsiung Lai
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  7. Chia-Hui Hung
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Corresponding authors

Correspondence to Chien-Hsing Lee or Chung-Yi Chen.

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Kuo, CN., Chen, CH., Chen, SN. et al. Anti-angiogenic effect of hexahydrocurcumin in rat corneal neovascularization. Int Ophthalmol 38, 747–756 (2018). https://doi.org/10.1007/s10792-017-0530-6

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  • DOI: https://doi.org/10.1007/s10792-017-0530-6

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