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Ascorbate oxidation stimulates rice root growth via effects on auxin and abscisic acid levels

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Abstract

Ascorbic acid (AA) and AA oxidation play a vital role in plant growth and development. In this study, we investigated the role of AA and AA oxidation in rice (Oryza sativa) root growth. Our results show, that rice AA biosynthesis mutant vitamin C 1 (vtc1) seedlings have a defect in radicle and early vegetative root growth. AA measurement displayed significantly lower levels of total AA, and mainly lower dehydroascorbic acid (DHA) in the roots of the vtc1 mutant. Phytohormone analysis shows that roots of the vtc1 mutant also contain lower levels of Indole-3-acetic acid (IAA) and abscisic acid (ABA). The vtc1 radicle root phenotype could be complemented by exogenous ABA or auxin (1- naphthalene acetic acid (NAA)) application, but not by AA application. Also, NAA and ABA treatments promoted radicle and early vegetative root growth similarly in WT as in the vtc1 mutant, implicating that they act downstream of AA biosynthesis. Both the radicle and the early vegetative root growth phenotype of vtc1 could be complemented by treatments with DHA or ascorbate oxidase (AO), the enzyme that oxidizes AA to DHA. Our data further demonstrate accumulation of IAA and ABA upon AO treatment in wildtype seedlings, implicating that AO-induced rice root growth is regulated via auxin and ABA levels. Taken together, these results imply that ascorbic acid and its oxidation stimulates rice root growth via positive effects on auxin and ABA levels.

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Data Availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Funding

This research was funded by the Special Research Fund (Bijzonder Onderzoeksfonds) of Ghent University through a BOF Starting Grant to Tina Kyndt.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Proeftuinstraat 86, B–9000, Ghent, Belgium

    Richard Raj Singh & Tina Kyndt

  2. Department of Green Chemistry and Technology, Research group EnVOC, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, B–9000, Ghent, Belgium

    Kristof Demeestere

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  1. Richard Raj Singh
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by RRS, KD and TK. The first draft of the manuscript was written by RRS and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Tina Kyndt.

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Singh, R.R., Demeestere, K. & Kyndt, T. Ascorbate oxidation stimulates rice root growth via effects on auxin and abscisic acid levels. Plant Growth Regul 103, 151–163 (2024). https://doi.org/10.1007/s10725-023-01096-9

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