Abstract
The present investigation explored the capability of priming treatment with 250 mg L−1 of humic acid (HA) to improve nano-ZnO tolerance of two rice cultivars seedlings (Zhu Liang You 06 and Qian You No. 1). The results showed that seed germination, seedling growth, total soluble protein, sugar and starch contents significantly improved by seed priming with HA especially under high nano-ZnO stress (500 and 750 mg L−1) as compared to unprimed seeds. In contrast, electrolyte leakage significantly increased after exposure to 500 and 750 mg L−1 nano-ZnO, but decreased obviously after HA priming in both cultivars. Abscisic acid (ABA) content in the seeds germinated under nano-ZnO stress was higher than those grown under non-stress condition; while gibberellins (GA) content decreased under nano-ZnO stress. HA priming down-regulated the relative expression levels of OsABA8ox2 and OsNCED1, which were key genes in ABA biosynthesis and catabolism of rice seeds. In contrast, up-regulation in OsGA20ox2 and OsGA3ox1 were induced by HA priming. The histochemical analysis reported that higher concentration of hydrogen peroxide (H2O2) and superoxide radical (O2·−) were observed in stressed roots with nano-ZnO as compared with non-stress condition, indicating reduction of root cell viability and severe oxidative burst. However, HA priming reduced obviously H2O2, O2·−, antioxidant enzymes activities (SOD, POD, CAT) and malondialdehyde (MDA) content of rice seedlings under stress. Significant increases in dehydroascorbate reductase (DHAR) and monodehydroascorbate reductase (MDAR) activities were observed in nano-ZnO stressed seedlings, but they were diminished evidently by HA priming. In addition, the improvement of HA on cell ultra structure of root tip and leaf mesophyll was detected under nano-ZnO stress especially 750 mg L−1 concentration. Therefore, it was suggested that HA priming could definitely improve the rice seed germination and seedling growth under nano-ZnO stress to some extent.
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References
Ashraf M, Foolad MR (2007) Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ Exp Bot 59:206–216
Asli S, Neumann PM (2010) Rhizosphere humic acid interacts with root cell walls to reduce hydraulic conductivity and plant development. Plant Soil 336:313–322
Blaylock MJ, Huang JW (2000) Phytoextraction of metals. In: Raskin I, Ensley BD (eds) Phytoremediation of toxic metals: using plants to clean up the environment. Wiley, Toronto, p 303
Bradford NM (1976) Rapid and sensitive method for quantitation of microgram quantities of protein utilizing principle of protein-dye binding. Anal Biochem 72:248–254
Chaoui A, Mazhoudi S, Ghorbal MH, Ferlani EE (1997) Cadmium and zinc induction of lipid peroxidation and effects on antioxidant enzyme activities in bean (Phaseolus vulgaris L.). Plant Sci 127: 139–147
Choudhury S, Panda SK (2005) Toxic effect, oxidative stress and ultrastructural changes in moss Taxitheelium nepalense (Schwaegr.) Broth. under lead and chromium toxicity. Water Air Soil Pollut 167:73–90
Daud MK, Variath MT, Ali S, Najeeb U, Muhammad J, Hayat Y, Dawood M, Khan MI, Zaffar M, Sardar AC, Tong XH, Zhu S (2009) Cadmium-induced ultramorphological and physiological changes in leaves of two transgenic cotton cultivars and their wild relative. J Hazard Mater 168:614–625
Daud MK, Quiling H, Lei M, Ali B, Zhu SJ (2015) Ultrastructural, metabolic and proteomic changes in leaves of upland cotton in response to cadmium stress. Chemosphere 120:309–320
Finch-Savage WE, Leubner-Metzger G (2006) Seed dormancy and the control of germination. New Phytol 171:501–523
Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48:909–930
Gill RA, Hu XQ, Ali B, Yang C, Shou JY, Wu YY, Zhou WJ (2014) Genotypic variation of the responses to chromium toxicity in four oilseed rape cultivars. Biol Plant 58:539–550
Halliwell B, Gutteridge JMC, Aruoma O (1987) The deoxyribose method: a simple ‘test tube’ assay for determination of rate constants for reactions of hydroxyl radicals. Anal Biochem 165:215–219
Hu Q, Fu Y, Guan, Y, Lin C, Cao D, Hu W, Sheteiwy M, Hu J (2016) Inhibitory effect of chemical combinations on seed germination and pre-harvest sprouting in hybrid rice. Plant Growth Regul 80:281–289
International Seed Testing Association (ISTA) (2004) Seed Sci Technol 24: 1–335
Jiang M, Zhang J (2003) Effect of abscisic acid on active oxygen species, antioxidative defence system and oxidative damage in leaves of maize seedlings. Plant Cell Physiol 42:1265–1273
Kool PL, Ortiz MD, Gastel VCA (2011) Chronic toxicity of ZnO nanoparticles, non-nano ZnO and ZnCl2 to Folsomia candida (Collembola) in relation to bioavailability in soil. Environ Pollut 159:2713–2719
Law MY, Charles SA, Halliwell B (1983) Glutathione and ascorbic-acid in spinach (Spinacia oleracea) chloroplasts, the effect of hydrogen-peroxide and of paraquat. Biochem J 210:899–903
Lesmana SO, Febriana N, Soetaredjo FE, Sunarso J, Ismadji S (2009) Studies on potential applications of biomass for the separation of heavy metals from water and waste water. Biochem Eng J 44:19–41
Lin D, **ng B (2007) Phytotoxicity of nanoparticles: inhibition of seed germination and root growth. Environ Pollut 150:243–250
Liu Y, Ye N, Liu R, Chen M, Zhang J (2010) H2O2 mediates the regulation of ABA catabolism and GA biosynthesis in Arabidopsis seed dormancy and germination. J Exp Bot 61:2979–2990
Mahmoud A, Ezgi O, Merve A, Ozhan G (2016) In vitro toxicological assessment of magnesium oxide nanoparticle exposure in several mammalian cell types. Int J Toxicol 35:429–437
Martins LL, Mourato MP, Baptista S, Reis R, Carvalheiro F, Almeida AM, Fevereiro P, Cuypers A (2014) Response to oxidative stress induced by cadmium and copper in tobacco plants (Nicotiana tabacum) engineered with the trehalose-6-phosphate synthase gene (AtTPS1). Acta Physiol Plant 3:755–765
Meloni A, Oliva MA, Martinez CA, Cambraia J (2003) Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Environ Exp Bot 49:69–76
Miyake C, Asada K (1992) Thylakoid-bound ascorbate peroxidase in spinach chloroplasts and photoreduction of its primary oxidation product monodehydroascorbate radicals in thylakoids. Plant Cell Physiol 33:541–553
Moghadam HRT (2013) Humic acid as an ecological pathway to protect corn plants against oxidative stress. Biological Forum 7:1704–1709.
Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplast. Plant Cell Physiol 22:867–880
Nambara E, Marion-Poll A (2005) Abscisic acid biosynthesis and catabolism. Plant Biol 56:165–185
Panda SK (2007) Chromium-mediated oxidative stress and ultrastructural changes in root cells of develo** rice seedlings. J Plant Physiol 164:1419–1428
Parera CA, Cantliffe DJ (1994) Pre-sowing seed priming. Hortic Rev 16:109–141
Pokhrel LR, Dubey B (2013) Evaluation of developmental responses of two crop plants exposed to silver and zinc oxide nanoparticles. Sci Total Environ 453:321–332
Qadir S, Qureshi MI, Javed S, Abdin MZ (2004) Genotypic variation in phytoremediation potential of Brassica juncea cultivars exposed to Cd stress. Plant Sci 167:1171–1181
Qian P, Sun R, Ali B, Gill RA, Xu L, Zhou WJ (2014) Effects of hydrogen sulfide on growth, antioxidative capacity, and ultrastructural changes in oilseed rape seedlings under aluminum toxicity. J Plant Growth Regul 33:526–538
Reddy AM, Kumar SG, Jyothsnakumari J, Thimmanaik S, Sudhakar C (2005) Lead induced changes in antioxidant metabolism of horsegram (Macrotyloma uniflorum (Lam.) Verdc.) and bengalgram (Cicer arietinum L.). Chemosphere 60:97–104
Schaedle M, Bassham JA (1977) Chloroplast glutathione reductase. Plant Physiol 59:1011–1012
Shaw AK, Zahed H (2013) Impact of nano-CuO stress on rice (Oryza sativa L.) seedlings. Chemosphere 93:906–915
Shaw AK, Supriya G, Hazem MK, Karolina B, Marian B, Marek Z, Zahed H (2014) Nano-CuO stress induced modulation of antioxidative defense and photosynthetic performance of Syrian barley (Hordeum vulgare L.). Environ Exp Bot 102:37–47
Sheteiwy MS, Guan Y, Cao D, Li J, Nawaz A, Hu Q, Hu W, Ning M, Hu J (2015) Seed priming with polyethylene glycol regulating the physiological and molecular mechanism in rice (Oryza sativa L.) under nano-ZnO stress. Sci Rep 5:14278
Sheteiwy MS, Fu Y, Hu Q, Nawaz A, Guan Y, Li Z, Huang Y, Hu J (2016) Seed priming with polyethylene glycol induces antioxidative defense and metabolic regulation of rice under nano-ZnO stress. Environ Sci Pollut Res 23:19989–20002
Sheteiwy M, Shen H, Xu J, Guan Y, Song W, Hu J (2017) Seed polyamines metabolism induced by seed priming with spermidine and 5-aminolevulinic acid for chilling tolerance improvement in rice (Oryza sativa L.) seedlings. Environ Exp Bot 137:58–72
Valipour M (2013a) Evolution of irrigation-equipped areas as share of cultivated areas. Irrig Drain Sys Eng 2:1
Valipour M (2013b) Use of surface water supply index to assessing of water resources management in Colorado and Oregon. US 3:631–640
Valipour M (2015) Land use policy and agricultural water management of the previous half of century in Africa. Appl Water Sci 5:367–395
Velikova V, Yordanov I, Edreva A (2000) Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Plant Sci 151:59–66
Viero DP, Valipour M (2017) Modeling anisotropy in free-surface overland and shallow inundation flows. Adv Water Res 104:1–14
Wu F, Zhang G, Dominy P (2003) Four barley genotypes respond differently to cadmium: lipid peroxidation and activities of antioxidant capacity. Environ Exp Bot 50:67–78
Yin L, Colman BP, McGill BM, Wright JP, Bernhardt ES (2012) Effects of silver nanoparticle exposure on germination and early growth of eleven wetland plants. PLoS ONE 7:1–7
Zhang W (2003) Nanoscale iron particles for environmental remediation: an overview. J Nanopart Res 5:323–332
Zhang H, Hu LY, Li P, Hu KD, Jiang CX, Luo JP (2010) Hydrogen sulfide alleviated chromium toxicity in wheat. Biol Plant 54:743–747
Zhou WJ, Leul M (1999) Uniconazole-induced tolerance of rape plants to heat stress in relation to changes in hormonal levels, enzyme activities and lipid peroxidation. Plant Growth Regul 27:99–104
Zhu JK (2000) Genetic analysis of plant salt tolerance using Arabidopsis. Plant Physiol 124:941–948
Zhu G, Ye N, Zhang J (2009) Glucose-induced delay of seed germination in rice is mediated by the suppression of ABA catabolism rather than an enhancement of ABA biosynthesis. Plant Cell Physiol 50:644–651
Zhu L, Cao D, Hu Q, Guan Y, Hu W, Nawaz A, Hu J (2015) Physiological changes and sHSPs genes relative transcription in relation to the acquisition of seed germination during maturation of hybrid rice seed. J Sci Food Agric 96:1–8
Acknowledgements
This research was supported by the National Natural Science Fund (Nos. 31201279, 31371708, 31671774), Zhejiang Provincial Natural Science Foundation (LZ14C130002, LY15C130002), the Project of the Science and Technology Department of Zhejiang Province (Nos. 2013C02005), Dabeinong Funds for Discipline Development and Talent Training in Zhejiang University and Jiangsu Collaborative Innovation Center for Modern Crop Production, P.R. China.
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Sheteiwy, M.S., Dong, Q., An, J. et al. Regulation of ZnO nanoparticles-induced physiological and molecular changes by seed priming with humic acid in Oryza sativa seedlings. Plant Growth Regul 83, 27–41 (2017). https://doi.org/10.1007/s10725-017-0281-4
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DOI: https://doi.org/10.1007/s10725-017-0281-4