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Assessment of genetic diversity of Camellia yuhsienensis based on leaf structure and inter simple sequence repeat (ISSR) markers

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Abstract

Genetic diversity provides valuable genetic resources and forms the basis of plant breeding. Plant leaves are closely connected to the external environment and play important roles in plant growth and development. Here, 13 cultivars of Camellia yuhsienensis were collected from Southern China to examine the magnitude of genetic diversity and differentiation, using cluster analysis based on 21 leaf traits and ten Inter simple sequence repeat (ISSR) markers. Morphological study indicated that all traits varied to different degrees, indicating a high degree of genetic diversity in C. yuhsienensis. Eight typical leaf characteristics were screened using principal component analysis, which showed that the selected traits were highly correlated and clustered. Average polymorphism percentage was obtained from the ISSR marker pattern using ten primers. A total of 201 loci were amplified, of which 175 were polymorphic with an average polymorphism percentage of 87.06%. Polymorphism information content (PIC), marker index (MI) and resolving power (RP) revealed that the selected primers were found to be informative in distinguishing C. yuhsienensis germplasm resources. Nei's diversity Index and the Shannon’s diversity index values indicated a certain level of genetic diversity. Cluster analysis results based on leaf structure and molecular markers were generally consistent, indicating a high degree of effective loci for morphological markers of C. yuhsienensis. Our results revealed genetic differences and commonalities among the cultivars, providing valuable information to identify appropriate seedling cultivars and conserve germplasm resources for future utilization.

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References

  • Budak H, Shearman RC, Parmaksiz I et al (2004) Comparative analysis of seeded and vegetative biotype buffalograsses based on phylogenetic relationship using ISSRs, SSRs, RAPDs, and SRAPs. Theor Appl Genet 109:280–288

    Article  CAS  PubMed  Google Scholar 

  • Cai DS, Pei XX, Min XX et al (2023) Comparative analysis of anatomical structure and photosynthetic characteristics of Camellia oleifera leaves in “Sanhua Series.” Anhui Agric Sci Bull 29:52–57

    Google Scholar 

  • Cao LQ, Zhong QP, Zou YL et al (2023) Leaf structure variations and relationship with environmental factors among germplasm resources of Vernicia fordii. J Nan**g for Univ (nat Sci Ed) 47:95–102

    Google Scholar 

  • Chen Y, Yuan DY, Li YM et al (2020) Floral morphological and breeding characteristics of the F1 generation of Camellia. J Fujian Agric for Univ (nat Sci Ed) 49:440–446

    Google Scholar 

  • Chen L (2020) Phenotype evaluation and analysis of genetic diversity based on SSR molecular markers of wild Camellia oleifera in **nxian. Dissertation. Jiangxi Agricultural University. (in Chinese)

  • Gong W, **ao S, Wang L et al (2022) Chromosome-level genome of Camellia lanceoleosa provides a valuable resource for understanding genome evolution and self-incompatibility. Plant J 110:881–898. https://doi.org/10.1111/tpj.15739

    Article  CAS  PubMed  Google Scholar 

  • Guo Y, Zhang SH, Li Y et al (2022) Diversity analysis of 36 leaf phenotypic traits of Chinese chestnut. Sci Agric Sin 55:991–1009

    Google Scholar 

  • He ZL, Liu CX, Wang XN et al (2020) Assessment of genetic diversity in Camellia oleifera Abel. accessions using morphological traits and simple sequence repeat (SSR) markers. Breed Sci 70:586–593. https://doi.org/10.1270/JSBBS.20066

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hu Y, Yang L, Gao C et al (2022) A comparative study on the leaf anatomical structure of Camellia oleifera in a low-hot valley area in Guizhou Province, China. PLoS ONE 17:e0262509. https://doi.org/10.1371/JOURNAL.PONE.0262509

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jiang Y, Yao XH, Cao YQ et al (2016) Variation and comprehensive evaluation of fruit quality of “Changlin” series of Camellia oleifera at different distribution regions. Nonwood Forest Research 34:42–48

    Google Scholar 

  • Khan RT, Abbas SR, Ahmed SD (2020) Assessment of genetic diversity of tomato genotypes by using molecular markers. Pure Appl Biol 9:1532–1540. https://doi.org/10.19045/bspab.2020.90160

    Article  CAS  Google Scholar 

  • Li J, Li DD, Wang L et al (2023) Analysis of leaf anatomical structure of 43 tea plant (Camellia sinensis) germplasm resources in Hanzhong. Acta Agric Boreali-Occidentalis Sin 32:62–71

    Google Scholar 

  • Li GS (2015) The research of ISSR on relationship 5 species of wild camellia of genus camellia and fingerprint construction. Dissertation. Central South University of Forestry & Technology Publishing. (in Chinese)

  • Lin P, Wang KL, Wang Y et al (2022) The genome of oil-Camellia and population genomics analysis provide insights into seed oil domestication. Genome Biol 23:14. https://doi.org/10.1186/s13059-021-02599-2

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lin P, Wang KL, Yao XH, Ren HD (2023) Development of DNA molecular ID in Camellia oleifera germplasm based on transcriptome-wide SNPs. Sci Agric Sin 56:217–235

    Google Scholar 

  • Peng FZ, Wu YY, Hao MZ et al (2012) Genetic diversity of Camellia oleifera using ISSR and SRAP markers. J Nan**g for Univ (nat Sci Ed) 36:19–25

    CAS  Google Scholar 

  • Qi HS, Sun XX, Yan WP et al (2022) Genetic relationships and low diversity among the tea-oil Camellia species in Sect. Oleifera, a bulk woody oil crop in China. Front Plant Sci 13:996731–996731. https://doi.org/10.3389/FPLS.2022.996731

    Article  PubMed  Google Scholar 

  • Ray A, Jena S, Haldar T et al (2019) Population genetic structure and diversity analysis in Hedychium coronarium populations using morphological, phytochemical and molecular markers. Ind Crops Prod 132:118–133. https://doi.org/10.1016/j.indcrop.2019.02.015

    Article  CAS  Google Scholar 

  • Ren W (2019) Genetic diversity and cross compatibility of elite genotypes in Camellia oleifera. Dissertation. Fujian Agriculture and Forestry University. (in Chinese)

  • Seyit AK, Erdogan EH, Emine P (2010) Comparison of effectiveness of ISSR and RAPD markers in genetic characterization of seized marijuana (Cannabis sativa L.) in Turkey. Afr J Agr Res 5:2925–2933

    Google Scholar 

  • Shen TF, Huang B, Xu M et al (2022) The reference genome of Camellia chekiangoleosa provides insights into Camellia evolution and tea oil biosynthesis. Hortic Res 9:3692–3702. https://doi.org/10.1093/hr/uhab083

    Article  Google Scholar 

  • Song QQ, Fu MW, He YF et al (2017) ISSR analysis of genetic diversity of Camellia chekiangoleosa Hu in Fujian province. J Sichuan Agric Univ 35:370–374

    Google Scholar 

  • Tan, (2023) Advances in the molecular breeding of Camellia oleifera. J Cent South Univ for Technol 43:1–24

    Google Scholar 

  • Xu Y, Huang L, Li JH et al (2022) Identification and genetic diversity analysis of olive cultivars in Longnan based on phenotype and SSR markers. For Res 35:33–43

    Google Scholar 

  • Yan CY, Li JX, He YM et al (2014) Genetic diversity of 30 Camellia oleifera germplasms by using SRAP marker. Genomics Appl Biol 33:617–623

    CAS  Google Scholar 

  • Yan C, Lin P, Lyu T et al (2018) Unraveling the roles of regulatory genes during domestication of cultivated Camellia: evidence and insights from comparative and evolutionary genomics. Genes 9:488. https://doi.org/10.3390/genes9100488

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Yan HQ, Qi HS, Li Y et al (2022) Assessment of the genetic relationship and population structure in oil-tea Camellia species using simple sequence repeat (SSR) markers. Genes 13:2162–2162. https://doi.org/10.3390/GENES13112162

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Yan M (2020) Genetic diversity analysis and regional applicability evaluation of Camellia oleifera varieties in Jiangxi province. Dissertation. Jiangxi Agricultural University. (in Chinese)

  • Yang Y, Yuan DY, Liu DM et al (2020) Effect of different pollination method on fruit setting rate and fruit growth of Camellia yubsienensis Hu. J Cent S Univ for Technol 36:61–65

    Google Scholar 

  • Yao XH, Huang Y, Ren HD et al (2014) Analysis on the leaf phenotype frequency distribution and diversity index of Camellia meiocarpa different populations. J Southwest for Univ 34:47–52

    Google Scholar 

  • Yu XY, Yu FY, Liu JB et al (2013) Identification and genetic diversity analysis of Camellia oleifera varieties using ISSR marker. J Nan**g for Univ (nat Sci Ed) 37:61–66

    CAS  Google Scholar 

  • Zhang P, Yang Y, ** RC et al (2018) Leaf phenotypic variation in populations of Camellia gauchowensis. For Environ Sci 34:13–19

    Google Scholar 

  • Zhang TJ (2019) Phenotypic and anatomical diversity of leaf of Davidia involucrata natural populations in Sichuan province. Dissertation. Sichuan Agricultural University. (in Chinese)

  • Zhou CC, Yang DM, Li SK et al (2020) Genetic relationship analysis of 18 Cerasus material based on inter-simple sequence repeat molecular marker. J for Environ 40:46–53

    Google Scholar 

  • Zhou LY (2014) ISSR analysis on the diversity of oil-tea camellia germplasms. Dissertation. Hunan Normal University. (in Chinese)

  • Zhu YL, Guo FG (2020) Genetic diversity analysis of Cotoneaster buxifolius in Yunnan Province based on phenotype characters and ISSR marker. For Res 33:19–26

    Google Scholar 

  • Zhu YZ, Liang DY, Song ZJ et al (2022) Genetic diversity analysis and core germplasm collection construction of Camellia oleifera based on fruit phenotype and SSR data. Genes 13:2351–2351. https://doi.org/10.3390/GENES13122351

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

We would like to thank Editage (www.editage.cn) for English language editing.

Funding

This research was supported by Fundamental Research Funds for the Central Non-profit Research Institution (CAFYBB2021MA003, CAFYBB2023ZA001-2, CAFYBB2019MB003).

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Authors and Affiliations

  1. Experimental Center of Subtropical Forestry, Chinese Academy of Forestry, Fenyi, 336600, Jiangxi, China

    Yuling Zou, ** Zhong, Danyang Chen, Zexin Chen, Yaqi Yuan, Hongyan Guo, Youcheng Zhou, **feng Wang & Linqing Cao

Authors
  1. Yuling Zou
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  2. **aoning Ge
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  3. Chao Yan
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  4. Qiu** Zhong
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  5. Danyang Chen
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  6. Zexin Chen
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  7. Yaqi Yuan
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  8. Hongyan Guo
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  9. Youcheng Zhou
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  10. **feng Wang
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  11. Linqing Cao
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Contributions

QZ and LC conceived and designed the study. DC, ZC, YY, HG and JW collected the samples. YZ, XG, CY and YZ collected and analyzed the data. YZ wrote the manuscript. LC revised the manuscript accordingly. All the authors have read and approved the final manuscript.

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Correspondence to Qiu** Zhong or Linqing Cao.

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Zou, Y., Ge, X., Yan, C. et al. Assessment of genetic diversity of Camellia yuhsienensis based on leaf structure and inter simple sequence repeat (ISSR) markers. Genet Resour Crop Evol (2024). https://doi.org/10.1007/s10722-024-01910-x

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