Abstract
Leaf color affects the efficiency of photosynthesis, and leaf color mutants are important genetic materials for studying the mechanisms of photosynthesis, chlorophyll biosynthesis, and chloroplast development in rice. In this study, a white-striped leaf mutant, wst1, was obtained from the mutant population of the indica restorer line ‘Chuanhui 907’ (R907) when treated with 60Co-γ radiation. Compared to the wild-type, the wst1 mutant showed normal leaf color before tillering and white stripes on the leaf and leaf sheaths after tillering. The chlorophyll and carotenoid contents were significantly reduced, and the thylakoids of chloroplasts developed abnormalities in wst1 plants in the tillering stage. The results of agronomic trait analysis showed that the number of effective panicles, plant height, seed setting rate, and 1000-grain weight of the wst1 mutant were significantly lower than those of the wild-type. Genetic analysis revealed that the phenotype of the wst1 mutant is controlled by a pair of recessive nuclear genes. The candidate gene was mapped to a 72 kb region between the InDel markers M6 and M12 on the short arm of chromosome 1 using molecular marker linkage analysis. Candidate genes were sequenced on the interval, and a G base was replaced by A at the 6972nd position on the 16th exon of LOC_Os01g01920, which encoded a previously reported protein containing the HD domain, WSF3/WFSL1, leading to alternative splicing, causing a 104 bp deletion in the coding region, and resulting in mistranslation after the 490 amino acid of the encoded protein translation in wst1. RT-qPCR analysis showed that the expression levels of most genes related to chlorophyll synthesis and chloroplast development were significantly altered in wst1 plants. Our study identified a novel allele of wsf3 and wfsl1 mutant and provided a new genetic resource and theoretical basis for further understanding of the molecular mechanism of WST1 gene regulation of white-striped leaves in rice.
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Acknowledgements
This study was supported by the National Natural Science Foundation of China-Youth Science Fund (32001531), Sichuan Science and Technology Program (2022ZDZX0012, and 2021YFYZ0016).
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This study was supported by the National Natural Science Foundation of China-Youth Science Fund (32001531), Sichuan Science and Technology Program (2022ZDZX0012, and 2021YFYZ0016).
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BH, ZP, and PW planned and designed the research. BH, ZH, XX, AD, HL, MW, LW, and CZ performed the experiments. LW and PW conducted fieldwork. BH, ZH, and ZP analyzed the data. BH and XX wrote the manuscript. All authors approved the manuscript.
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Hu, B., He, Z., ** of a white stripe leaf mutant in rice. Genet Resour Crop Evol (2024). https://doi.org/10.1007/s10722-024-01897-5
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DOI: https://doi.org/10.1007/s10722-024-01897-5