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Screening wild Brassica species against Alternaria brassicicola (Schw.) Wiltsh for breeding Alternaria leaf spot resistance in Brassica vegetables

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Abstract

Alternaria leaf spot is a major disease of Brassica crops, and it causes huge economic loss to both the cultivated Brassica oilseeds and vegetables. The present study was aimed to develop a non-destructive robust method for screening of wild Brassica species and to find resistant wild species against Alternaria brassicicola (Schw.) Wiltsh. For this, 38 wild species of Brassicaceae were screened at adult plant stage by an in vitro detached leaf inoculation method in three consecutive years i.e. 2019–2020, 2020–2021 and 2021–2022. The new screening protocol created favourable environment (temperature 25 ± 2 °C; relative humidity > 90%) for the pathogen by using incubator. The host leaves were retained in turgid condition by placing sucrose 5% w/v on slanting cut at petiolar base. The consistency in reactions of the wild species against A. brassicicola during all three years of testing indicates the robustness of the protocol. Multiple parameters were recorded on leaf condition and disease response of the wild species and devised a score system for disease rating. Complete resistance was observed in Capsella while resistance in Lepidium, Camelina and Biscutella. Both Capsella bursa pastoris (L.) Medik (early) and C. bursa pastoris (late) were symptomless resistant. Camelina sativa (L.) Crantz, Diplotaxis erucoides (L.) DC and Diplotaxis gomez-campoi Mart.-Laborde were found to be resistant against A. brassicicola. The significant correlation between disease parameters indicates the robustness and effectiveness of the screening protocol for wild Brassica species.

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References

  • Ahmad A, Ashraf Y (2016) In vitro and in vivo management of Alternaria leaf spot of Brassica campestris L. J Plant Pathol Microbiol 7:365. https://doi.org/10.4172/2157-7471.1000365

    Article  CAS  Google Scholar 

  • Ansar M, Ghatak G (2018) Occurrence of Alternaria brassicae in seed-producing cauliflower and its role of seed infection. Int J Plant Sci 13(1):67–70

    Article  Google Scholar 

  • Browne LM, Conn KL, Ayer WA, Tewari JP (1991) The camalexins: new phytoalexins produced in the leaves of Camelina sativa (Cruciferae). Tetrahedron 47:3909–3914

    Article  CAS  Google Scholar 

  • Buchwaldt L, Green H (1992) Phytotoxicity of destruxin B and its possible role in the pathogenesis of Alternaria brassicae. Plant Pathol 41(1):55–63

    Article  CAS  Google Scholar 

  • Chrungu B, Verma N, Mohanty A, Pradhan A, Shivanna KR (1999) Production and characterization of interspecific hybrids between Brassica maurorum and crop Brassicas. Theor Appl Genet 98:608–613

  • Conn KL, Tewari JP (1989) Interaction of Alternaria brassicae conidia with leaf epicuticular wax of canola. Mycol Res 93(2):240–242

    Article  Google Scholar 

  • Conn KL, Tewari JP, Dahiya JS (1988) Resistance to Alternaria brassicae and phytoalexin-elicitation in rapeseed and other crucifers. Plant Sci 56(1):21–25

    Article  CAS  Google Scholar 

  • Corlett M, MacLatchy IA (1996) Alternaria brassicicola. Can J Plant Pathol 18(4):484–485

    Article  Google Scholar 

  • Deep S, Sharma P (2012) Host age as predisposing factor for incidence of black leaf spot of cauliflower caused by Alternaria brassicicola. Indian Phytopathol 65:71–75

    Google Scholar 

  • Dhingra M, Arora N, Aujla IS (2013) Alternaria. Agric Sci Digest 33(2):92–97

    CAS  Google Scholar 

  • Dickson MH, Petzoldt R (1993) Plant age and isolate source affect expression of downy mildew resistance in broccoli. HortScience 28:730–731

    Article  Google Scholar 

  • Doullah MAU, Meah MB, Okazaki K (2006) Development of an effective screening method for partial resistance to Alternaria brassicicola (dark leafspot) in Brassica rapa. Eur J Plant Pathol 116(1):33–43

    Article  Google Scholar 

  • Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12(1):13–15

  • Downey RK, Rimmer SR (1993) Agronomic improvements in oilseed Brassicas. Adv Agron 50:1–66

    Article  Google Scholar 

  • Hansen LN, Earle ED (1997) Somatic hybrids between Brassica oleracea L. and Sinapis alba L. with resistance to Alternaria brassicae (Berk.) Sacc. Theor Appl Genet 94(8):1078–1085

    Article  Google Scholar 

  • Hong CX, Fitt BDL, Welham SJ (1996) Effects of wetness period and temperature on development of dark pod spot (Alternaria brassicae) on oilseed rape (Brassica napus). Plant Pathol 45:1077–1089

    Article  Google Scholar 

  • Hussain A, Thakur RN (1963) Some sources of resistance to Alternaria blight of rapeseed and mustard. Indian Oilseed J 7:259–261

  • Kolte SJ (1985) Diseases of Annual Edible Oilseed Crops, vol II. CRC Press Inc., Boca Raton, p 11

    Google Scholar 

  • Kumar D, Maurya N, Bharti YK, Kumar A, Kumar K, Srivastava K et al (2014) Alternaria blight of oilseed brassicas: a comprehensive review. Afr J Microbiol Res 8:2816–2829

    Article  Google Scholar 

  • Kumari P, Bisht DS, Bhat SR (2018) Stable, fertile somatic hybrids between Sinapis alba and Brassica juncea show resistance to Alternaria brassicae and heat stress. Plant Cell Tissue Organ Cult 133:77–86

  • Lawrence CB, Mitchell TK, Craven KD, Cho Y, Cramer RA Jr, Kim KH (2008) At death’s door: Alternaria pathogenicity mechanisms. Plant Pathol J 24(2):101–111

    Article  CAS  Google Scholar 

  • MacKinnon SL, Keifer P, Ayer WA (1999) Components from the phytotoxic extract of Alternaria brassicicola, a black spot pathogen of canola. Phytochemistry 51:215–221

    Article  CAS  Google Scholar 

  • Meena PD, Meena RL, Chattopadhyay C, Kumar A (2004) Identification of critical stage for disease development and biocontrol of Alternaria blight of Indian mustard (Brassica juncea). J Phytopathol 152:204–209

    Article  Google Scholar 

  • Meena PD, Jambhulkar SJ, Gupta R, Meena HS, Singh D (2016) Rapid screening technique for Alternaria blight resistance in Indian mustard (Brassica juncea L.) using cotyledonary leaf method. J Plant Pathol 98(3):463–469

    Google Scholar 

  • Michereff SJ, Noronha MA, Xavier Filha MS, Câmara MP, Reis A (2012) Survey and prevalence of species causing Alternaria leaf spots on Brassica species in Pernambuco. Hortic Bras 30:345–348

    Article  Google Scholar 

  • Mondal KK, Chatterjee SC, Viswakarma N, Bhattacharya RC, Grover A (2003) Chitinase-mediated inhibitory activity of Brassica transgenic on growth of Alternaria brassicae. Curr Microbiol 47(3):0171–0173

    Article  CAS  Google Scholar 

  • Nowakowska M, Wrzesinska M, Kaminski P, Szczechura W, Lichocka M, Tartanus M, Kozik EU, Nowicki M (2019) Alternaria brassicicola–Brassicaceae pathosystem: insights into the infection process and resistance mechanisms under optimized artificial bio-assay. Eur J Plant Pathol 153:131–151

  • Nowicki M, Nowakowska M, Niezgoda A, Kozik E (2012) Alternaria black spot of crucifers: symptoms, importance of disease, and perspectives of resistance breeding. Veg Crops Res Bull. https://doi.org/10.2478/v10032-012-0001-6

    Article  Google Scholar 

  • Prasad L, Vishunavat K (2004) Detection of Alternaria brassicae, Alternaria brassicicola and their transmission through seed to seedling and to plants in cauliflower. Seed Res 32(1):84–88

  • Prasad L, Vishnuvat K (2006) Assessment of yield loss in cauliflower seed crop due to Alternaria blight. Indian Phytopathol 59:185–189

    Google Scholar 

  • Pryor BM, Michallides TJ (2002) Morphological, pathogenic, and molecular characterization of Alternaria isolates associated with Alternaria late blight of pistachio. Phytopathology 92(4):406–416

    Article  CAS  PubMed  Google Scholar 

  • Ryschka U, Schumann G, Klocke E, Scholze P, Neumann M (1996) Somatic hybridization in Brassicaceae. Acta Hort 407:201–204

    Article  Google Scholar 

  • Saha P, Kalia P, Sharma M, Singh D (2016) New source of black rot disease resistance in Brassica oleracea and genetic analysis of resistance. Euphytica 207:35–48

  • Scholze P (2002) In-vitro-germinability of conidia and differences in aggressivity in Alternaria brassicicola isolates on Brassicaceous vegetables (Brassica oleracea L.). Pflanzenschutz Ber 60:105–114

    Google Scholar 

  • Sharma BR, Dhiman JS, Thakur JC, Singh A, Bajaj KL (1995) Multiple disease resistance in cauliflower. Adv Hortic Sci 5(1):30–34

    Google Scholar 

  • Sharma G, Dinesh Kumar V, Haque A et al (2002) Brassica coenospecies: a rich reservoir for genetic resistance to leaf spot caused by Alternaria brassicae. Euphytica 125:411–417

    Article  Google Scholar 

  • Sharma P, Sharma SR, Sindhu M (2004) A detached leaf technique for evaluation of resistance in cabbage and cauliflower against three major pathogens. Indian Phytopath 57:315–318

    Google Scholar 

  • Sharma P, Deep S, Sharma M, Bhati DS (2012) Genetic variation of Alternaria brassicae (Berk) Sacc causing dark leaf spot of cauliflower and mustard in India. J Gen Plant Pathol 79:41–45

    Article  Google Scholar 

  • Shi X, Zeng K, Wang X, Liang Z, Wu X (2021) Characterization of Alternaria species causing leaf spot on Chinese cabbage in Shanxi province of China. J Plant Pathol 103(1):283–293

    Article  Google Scholar 

  • Shrestha SK, Munk L, Mathur SB (2005) Role of weather on Alternaria leaf blight disease and its effect on yield and yield components of mustard. Nepal Agric Res J 6:62–72

    Article  Google Scholar 

  • Singh DA, Prasad RA, Yadav SP, Kumar SO (2014) Characterization of Alternaria brassicae causing Alternaria blight of rapeseed-mustard (Brassica juncea L.) and its management. Suppl Plant Pathol. 9(3):1207–1212.

  • Singh S, Kalia P (2021) Advances in cauliflower (Brassica oleracea var. botrytis L.) breeding, with emphasis on India. Advances in plant breeding strategies: vegetable crops: Volume 10: leaves, flowerheads, green pods, mushrooms and truffles, pp 247–301

  • Singh N, Palat P, Dabas MR, Chandra DR (2008) Screening of germplasm for Alternaria leaf spot resistance in yellow sarson. Int J Plant Prot 1(2):116–117

    Google Scholar 

  • Singh S, Sharma SR, Kalia P, Sharma P, Kumar V, Kumar R et al (2013) Screening of cauliflower (Brassica oleracea L. var. botrytis L.) germplasm for resistance to downy mildew [Hyaloperonospora parasitica Constant (Pers.: Fr) Fr.] and designing appropriate multiple resistance breeding strategies. J Hort Sci Biotechnol 88(1):103–109

    Article  Google Scholar 

  • Singh S, Kalia P, Sharma SR (2022) New sources of downy mildew resistance in cauliflower and their prospects in resistance breeding. Eur J Plant Pathol 163(4):813–826

  • Tewari JP, Conn KL (1993) Reactions of some wild crucifers to Alternaria brassicae. Bull OILS SROP 16:53–58

    Google Scholar 

  • Vishwanath, Kolte SJ (1999) Methods of inoculation for resistance to Alternaria blight of rapeseed and mustard. J Mycol Plant Pathol 29:96–99

    Google Scholar 

  • Vishwanath KSJ, Singh MP, Awasthi RP (1999) Induction of resistance in mustard (Brassica juncea) against Alternaria black spot with an avirulent Alternaria brassicae isolate-D. Eur J Plant Pathol 105:217–220

    Article  Google Scholar 

  • Westman AL, Kresovich S, Dickson MH (1999) Regional variation in Brassica nigra and other weedy Crucifers for disease reaction to Alternaria brassicicola and Xanthomonas campestris pv. campestris. Euphytica 106:253–259

    Article  Google Scholar 

  • Wheeler BEJ (1969) An introduction to plant diseases. John Wiley and Sons Ltd., London

    Google Scholar 

  • White TJ, Bruns T, Lee SJWT, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: A Guide to Methods and Applications, vol 18, no 1, pp 315–322

  • Zhang K, Yuan-Ying S, Cai L (2013) An optimized protocol of single spore isolation for fungi. Cryptogam Mycol 34(4):349–356

    Article  CAS  Google Scholar 

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Acknowledgements

The authors are grateful to DST-SERB for financial support for the project ‘Map** and marker assisted breeding for Alternaria leaf spot resistance in Indian cauliflower (ECR/2017/003136) and the director, IARI, New Delhi for facilities.

Funding

The senior author acknowledges DBT-SERB for funding the study through Research grant No. ECR/2017/003136.

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Authors and Affiliations

  1. Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Shrawan Singh, Sushma Sagar & Sandhya Saroha

  2. ICAR-National Institute of Plant Biotechnology, New Delhi, 110012, India

    Mahesh Rao & Pooja Garg

  3. Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Lakshman Prasad

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  1. Shrawan Singh
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  2. Sushma Sagar
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Contributions

SS1 conceptualization, supervision and draft preparation; SS2 performed screening experiment and draft preparation; MR maintained and provided wild Brassica species and edited manuscript; SS3 and PG managed plants and brought samples; LP helped in inoculum preparation and edited manuscript.

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Correspondence to Shrawan Singh.

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Singh, S., Sagar, S., Rao, M. et al. Screening wild Brassica species against Alternaria brassicicola (Schw.) Wiltsh for breeding Alternaria leaf spot resistance in Brassica vegetables. Genet Resour Crop Evol (2023). https://doi.org/10.1007/s10722-023-01776-5

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