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Dominant gene for common bean resistance to common bacterial blight caused by Xanthomonas axonopodis pv. phaseoli

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Abstract

The common bacterial blight pathogen [Xanthomonas axonopodis pv. phaseoli (Xap)] is a limiting factor for common bean (Phaseolus vulgaris L.) production worldwide and resistance to the pathogen in most commercial cultivars is inadequate. Variability in virulence of the bacterial pathogen has been observed in strains isolated from Puerto Rico and Central America. A few common bean lines show a differential reaction when inoculated with different Xap strains, indicating the presence of pathogenic races. In order to study the inheritance of resistance to common bacterial blight in common bean, a breeding line that showed a differential foliar reaction to Xap strains was selected and was crossed with a susceptible parent. The inheritance of resistance to one of the selected Xap races was determined by analysis of segregation patterns in the F1, F2, F3 and F4 generations from the cross between the resistant parent PR0313-58 and the susceptible parent ‘Rosada Nativa’. The F1, F2 and F3 generations were tested under greenhouse conditions. Resistant and susceptible F3:4 sister lines were tested in the field. The statistical analysis of all generations followed the model for a dominant resistance gene. The resistant phenotype was found to co-segregate with the SCAR SAP6 marker, located on LG 10. These results fit the hypothesis that resistance is controlled by a single dominant gene. The symbol proposed for the resistance gene is Xap-1 and for the bacterial race, XapV1.

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Acknowledgments

Financial support for research at the Agricultural Experiment Station, University of Puerto Rico, Mayagüez campus was provided by the Hatch project 351 “Genetic improvement of beans for yield, pest resistance and food value”. The authors wish to thank Ms. R. Gaud for technical assistance during the study and to Dr. Linda Beaver and Dr. Carlos Ortiz for reviewing the manuscript.

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Authors and Affiliations

  1. Department of Crops and Agro-Environmental Sciences, University of Puerto Rico, Mayagüez, PR, 00681-9000, USA

    Mildred Zapata & James S. Beaver

  2. USDA-ARS Tropical Agriculture Research Station, Mayagüez, PR, 00680, USA

    Timothy G. Porch

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  1. Mildred Zapata
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  2. James S. Beaver
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  3. Timothy G. Porch
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Correspondence to Mildred Zapata.

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Zapata, M., Beaver, J.S. & Porch, T.G. Dominant gene for common bean resistance to common bacterial blight caused by Xanthomonas axonopodis pv. phaseoli . Euphytica 179, 373–382 (2011). https://doi.org/10.1007/s10681-010-0313-x

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