Abstract
Wastewater from shrimp farming is rich in organic material, solids, and nutrients, which cause a series of environmental problems when released into the environment. Currently, for the removal of nitrogen compounds from wastewater, among the most studied methods is biological denitrification. The objective of this study was to evaluate the operational parameters for the development of a more sustainable technology for the removal of nitrogen compounds from shrimp farm wastewater, using Bambusa tuldoides (a species of bamboo) as a source of carbon and a material conducive to the development of selected denitrifying bacteria. To optimize the process, biological denitrification assays were performed varying the following parameters: bamboo length (cm), pH, temperature, and stoichiometric proportions of C and N. The operational stability of the process with the reuse of the bamboo biomass was also evaluated. Cronobacter sakazakii and Bacillus cereus were identified as denitrifying microorganisms present in reactor with bamboo biomass. The best operational conditions observed were pH 6 to 7 and temperature 30 to 35 °C, and the addition of an external carbon source was not necessary for the denitrification process to occur efficiently. Under these conditions, biological denitrification occurred with an average efficiency above 90% based on the removal of the nitrogen contaminants evaluated (NO3˗N and NO2-N). Regarding operational stability, 8 cycles were performed using the same source of carbon without reducing the efficiency of the process.
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The dataset utilized/analyzed during the current study will be available from the corresponding author upon request.
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Acknowledgements
This study was partially financed by the Coordination for Foundation to support research and innovation in the state of Santa Catarina (FAPESC), Ânima Institute, Federal University of Rio Grande do Sul and Technological Center-University of the South of Santa Catarina (UNISUL) that we deeply thank.
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Jéssica Mendonça Ribeiro Cargnin developed the methodology and wrote the first draft of the manuscript, did the experiments, and organized the structure of the manuscript.
Harry Luiz Pilz Júnior did the investigation for microbiological identification.
Jair Juarez João did the conceptualization and supervision, analyzed the data, and edited the final draft of the manuscript.
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Cargnin, J.M.R., Júnior, H.P. & João, J.J. Sustainable technology: potential of biomass (Bambusa tuldoides) for biological denitrification of wastewater generated in shrimp farming. Environ Monit Assess 195, 736 (2023). https://doi.org/10.1007/s10661-023-11351-1
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DOI: https://doi.org/10.1007/s10661-023-11351-1