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Biocontrol efficacy of antagonists Trichoderma and Bacillus against post-harvest diseases in mangos

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Abstract

Stem-end and anthracnose fruit rot diseases caused by Lasiodiplodia theobromae and Colletotrichum gloeosporioides are devastating post-harvest diseases occurring in mangos and many other economically important fruit crops. In this study, the marine antagonists Trichoderma and Bacillus isolated from marine sponges and sea fans displayed potent biocontrol activity against both diseases on mango cultivars Nam Dok Mai Si Thong and Nam Dok Mai. Mangos were exposed to a spore suspension of T. asperellum KUFA 0042 at a concentration of 106 spores mL−1 or a crude extract of the same strain at a concentration of 10 g L−1 through a dip** process and then inoculated with pathogens. Remarkably, there was a significant suppression (p < 0.05) of lesion development caused by L. theobromae, with a reduction rate reaching up to 95%. Similarly, the treatment significantly reduced the lesion development of anthracnose disease caused by C. gloeosporioides by up to 93%. Additionally, mangos treated with a crude extract of marine B. subtilis KUFA 0163 at a concentration of 10 g L−1 also showed a reduction in the incidences of both stem-end rot and anthracnose diseases, with disease suppression of up to 94%. The spore suspensions of Trichoderma, Bacillus, and their crude extracts had no effect on mango physiology. This study’s results show the potential of marine-derived Trichoderma and Bacillus strains as promising candidates for the development of novel biocontrol agents. These could effectively manage post-harvest diseases in mango crops without impacting the fruit's physiology

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Acknowledgements

This work was financially supported by the Office of the Ministry of Higher Education, Science, Research and Innovation; and the Thailand Science Research and Innovation through the Kasetsart University Reinventing University Program 2021 and National Research Council of Thailand (NRCT), grant no. N41A640083.

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Authors and Affiliations

  1. Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok, 10900, Thailand

    Yarida Nujthet & Tida Dethoup

  2. Faculty of Science and Technology, Phranakhon Si Ayutthaya Rajabhat University, Ayutthaya, 13000, Thailand

    Chutima Kaewkrajay

  3. ICBAS—Instituto de Ciências Biomédicas Abel Salazar and CIIMAR, Rua de Jorge Viterbo Ferreira, 228, 4050–313, Porto, Portugal

    Anake Kijjoa

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  1. Yarida Nujthet
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Correspondence to Tida Dethoup.

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Nujthet, Y., Kaewkrajay, C., Kijjoa, A. et al. Biocontrol efficacy of antagonists Trichoderma and Bacillus against post-harvest diseases in mangos. Eur J Plant Pathol 168, 315–327 (2024). https://doi.org/10.1007/s10658-023-02757-1

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