Abstract
Current phytosanitary (import) measures for the false codling moth, Thaumatotibia leucotreta (Lepidoptera: Tortricidae) and the cotton bollworm moth Helicoverpa armigera (Lepidoptera: Noctuidae), do not provide protection to countries located in southern and central portions of the European Union (EU). Only glasshouses in northern parts of the EU benefit from EU wide phytosanitary regulation. The primary pathway for introduction of T. leucotreta and H. armigera into glasshouses in northern parts of the EU is via the import of cut flowers such as roses and to a lesser extent natural spread or migration. A limitating factor to the management and control of T. leucotreta and H. armigera is accurate identification. The aim of this study was to develop a loop-mediated isothermal amplification (LAMP) assay for specific identification of T. leucotreta and H. armigera.
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Acknowledgements
This work was funded by the Dutch Ministry of Agriculture, Nature and Food Economic Affairs and the private partners (Rijk Zwaan, Florensis, Anthura, Kwaliteits Controle Bureau, ENZA Zaden, Syngenta, BASF Vegetable Seeds, Agrifirm Plant, Monsanto Holland, Bejo Zaden, VGB, NVWA) in the Topsector Program “Horticulture and Starting Materials” under theme “Plant Health” nr. KV 1406-038. The authors want to thank Mrs. Maja vd Straten (NVWA) for supplying the insect specimens used in this study and the KCB inspection service to test the LAMP assays.
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Griekspoor, Y., Kurm, V., Jakomin, T. et al. Development of an on-site LAMP assay for identification of Thaumatotibia leucotreta and Helicoverpa armigera larvae on rose. Eur J Plant Pathol 165, 593–601 (2023). https://doi.org/10.1007/s10658-022-02619-2
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DOI: https://doi.org/10.1007/s10658-022-02619-2