Abstract
Miscanthus sinensis (Poaceae) is typical of many dominant grasses of East Asia. Due to its characteristics of fast growth and high biomass, Miscanthus, a C4 plant, has been long explored for the potential usage as biofuel. In this study, we described the development of nine microsatellite loci from M. sinensis for genetic studies. These new markers were tested in 25 individuals of five populations in three varieties. The number of alleles ranged from 3 to 8. The expected (H E) and observed (H O) heterozygosities were 0.37–0.78 and 0.05–0.52, respectively. All microsatellite loci are significantly deviated from Hardy–Weinberg expectations likely due to the population structure within samples. Linkage disequilibrium between four loci pairs were observed from the total 36 pairwise comparisons of loci. Cross-species transferability revealed that all loci can be applied to its closely related species, M. floridulus.
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References
Chiang YC, Schaal BA, Chou CH, Huang S, Chiang TY (2003) Contrasting selection modes at the Adh1 locus in outcrossing Miscanthus sinensis vs. inbreeding Miscanthus condensatus (Poaceae). Am J Bot 90:561–570. doi:10.3732/ajb.90.4.561
Chou CH, Chiang YC, Chiang ZY (2000) Genetic variability and phytogeography of Miscanthus sinensis var. condensatus, an apomictic grass, based on a RAPD fingerprints. Can J Bot 78:1262–1268. doi:10.1139/cjb-78-10-1262
Cifarelli RA, Gallitelli M, Cellini F (1995) Random amplified hybridization microsatellites (Rahm): isolation of a new class of microsatellite-containing DNA clones. Nucleic Acids Res 23:3802–3803. doi:10.1093/nar/23.18.3802
Clifton-Brown JC, Stampel PF, Jones MB (2004) Miscanthus biomass production for energy in Europe and its potential contribution to decreasing fossil fuel emissions. Glob Chang Biol 10:509–518. doi:10.1111/j.1529-8817.2003.00749.x
Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15
Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50
Hsu KC, Wang JP, Chen XL, Chiang TY (2004) Isolation and characterization of microsatellite loci in Acrossocheilus paradoxus (Cyprinidae) using PCR-based isolation of microsatellite arrays (PIMA). Conserv Genet 5:113–115. doi:10.1023/B:COGE.0000014051.67562.55
Huang CC, Chiang TY, Hsu TW (2008) Isolation and characterization of microsatellite loci in Taxus sumatrana (Taxaceae) using PCR-based isolation of microsatellite arrays (PIMA). Conserv Genet 9:471–473. doi:10.1007/s10592-007-9341-z
Lunt DH, Hutchinson WF, Carvalho GR (1999) An efficient method for PCR-based isolation of microsatellite arrays (PIMA). Mol Ecol 8:891–893. doi:10.1046/j.1365-294X.1999.00636.x
Raymond M, Rousset F (1995) Genepop (version-1.2)—population genetics software for exact tests and ecumenicism. J Hered 86:248–249
Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386
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Kuo-Hsiang Hung and Tzen-Yuh Chiang are contributed equally.
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Hung, KH., Chiang, TY., Chiu, CT. et al. Isolation and characterization of microsatellite loci from a potential biofuel plant Miscanthus sinensis (Poaceae). Conserv Genet 10, 1377–1380 (2009). https://doi.org/10.1007/s10592-008-9699-6
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DOI: https://doi.org/10.1007/s10592-008-9699-6