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Bulk industrial textile production of bio scouring for cellulosic fabric utilizing indigenous hot springs triacylglycerol acylhydrolases from Bacillus toyonensis and Bacillus thuringiensis

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Abstract

A sustainable approach in all industrial processes is prerequisite in industrial revolution 5 and 6. The need for optimization of all industrial products with less greenhouse gas emissions is beneficial for a better-quality product too. Bio scouring is a sustainable alternative to traditional scouring methods for cellulosic (cotton) fabrics. This research aimed to identify optimal parameters for employing triacylglycerol acylhydrolase (EC 3.1.1.3 Lipase) from Bacillus toyonensis and Bacillus thuringiensis consortium sourced from indigenous hot springs. To optimize the process time, temperature, and pH, the RSM was applied. The experimental ranges for temperature, reaction time, and pH were 6–8, 60–80 °C, and 50–150 min, respectively. The focus of the study was directed towards seven outcomes: Tensile strength warp and weft, tear strength warp and weft, wettability, whiteness index, and weight loss. Through a systematic experimental approach, the study determined the optimal parameter settings for the independent variables, setting the temperature at 70 °C, reaction time at 50 min, and pH at 8. The accuracy of the combination of variables was confirmed by subsequent validation experiments, which produced significant results. This validated the efficacy of the enzyme-assisted bio-scouring approach in enhancing cotton fabric properties while adhering to environmentally friendly practices with comparatively less GHG emissions.

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Funding

The research was funded by the Department of Botany and ORIC, Government College University, Lahore, Pakistan.

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Contributions

TB; Student, researcher trials, RSM software and applications, TI; Main supervisor, RSM designing, Methodology, total research plan, RSM, results interpretations, HM; Co supervisor, bio scouring process, production, bulk industrial textile trials, fabric testing, paper write up, results interpretations.

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Correspondence to Tehreema Iftikhar or Hammad Majeed.

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There was no conflict of interest acknowledged by the authors.

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This research does not involve any human or animal trials. The other ethics were reviewed and approved by the departmental research approval committee.

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Bashir, T., Iftikhar, T. & Majeed, H. Bulk industrial textile production of bio scouring for cellulosic fabric utilizing indigenous hot springs triacylglycerol acylhydrolases from Bacillus toyonensis and Bacillus thuringiensis. Cellulose 31, 1353–1381 (2024). https://doi.org/10.1007/s10570-023-05666-y

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