Abstract
Cellulose-based nanofibers are a versatile material with broad applications, including wound-healing/dressing biomaterials, food packing, water treatments, sensors, and energy storage materials. In this work, a cellulose acetate (CA) nanofiber membrane obtained from the electrospinning process was hydrolyzed to a regenerated cellulose (RC) nanofiber membrane. The RC nanofiber membrane coupled with Reactive Orange 4 (RO4) as the dye ligand was used in the purification of malate dehydrogenase (MDH) from the disrupted baker’s yeast in a single step. The optimal pH and the immobilized dye density for MDH adsorption were at pH 7.5 and ~ 520 mg dye/g membrane, respectively. The maximum equilibrium binding capacity for MDH was 3985.65 U/g, and the surface reaction was the main rate-limiting step for MDH transport to the nanofiber membrane throughout the adsorption process. The purification of the MDH process was performed by using a membrane contactor (i.d. 25 mm) in a flow system. An elution solution (pH 5) made of 10 mM NADH and 0.1 M NaCl resulted in high efficiency of MDH elution, recording a recovery yield of 89% and a purification factor of 78 folds. Similar performance of MDH purification was obtained using a larger membrane contactor (i.d. 47 mm), thereby proving the linear scalability of the purification process.
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Acknowledgments
YKC gratefully acknowledges the financial support provided by the Ministry of Science and Technology of Taiwan (Grant numbers MOST 107-2622-E-131-005-CC3 and 107-2221-E-131-013-).
Funding
Research grants from the Ministry of Science and Technology, Taiwan (Grant no. MOST 109–2622-E-131–004-CC3) supports salaries, equipment, supplies, reimbursement for attending symposia, and other expenses related to this research.Ministry of Science and Technology,Taiwan,MOST 107-2622-E-131-005-CC3,Yu-Kaung Chang,MOST 107-2221-E-131-013-,Yu-Kaung Chang
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SJ, J Investigation, Data curation, Formal analysis. S S S W: Writing—review & editing. C W O: Writing—review & editing, Conceptualization. B C H: Writing—review & editing. Y R L: Data curation, Formal analysis. C Y C: Formal analysis, Writing—review & editing. M H: Funding acquisition K H C: Formal analysis, Supervision, Writing—review & editing, Project administration. Y K C: Conceptualization, Supervision, Writing—review & editing, Project administration.
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Jian, SJ., Wang, S.SS., Ooi, C.W. et al. Cellulose-based nanofiber membrane functionalized with dye affinity ligand for purification of malate dehydrogenase from Saccharomyces cerevisiae. Cellulose 29, 9251–9281 (2022). https://doi.org/10.1007/s10570-022-04815-z
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DOI: https://doi.org/10.1007/s10570-022-04815-z