SpringerLink
Log in

Preparation and characterization of bamboo fiber/chitosan/nano-hydroxyapatite composite membrane by ionic crosslinking

  • Original Research
  • Published:
Cellulose Aims and scope Submit manuscript

Abstract

To obtain an ideal guided bone tissue regeneration (GBR) membrane, carboxylated bamboo fiber (BF) was designed to be introduced into the chitosan/nano-hydroxyapatite (CS/n-HA) system by ionic crosslinking to fabricate a novel BF/CS/n-HA composite membranes with different weight ratios. The formation mechanism of the BF/CS/n-HA ternary composite membrane and the effect of BF different contents on the BF/CS/n-HA composite membranes were studied by Fourier Transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), electromechanical universal tester, in vitro soaking in simulated body fluid (SBF). Results demonstrated that CS and modified BF would be ionic crosslinked by electrostatic interaction to form a three dimensional polyelectrolyte structure, meanwhile, n-HA was loaded in the polyelectrolyte structure by hydrogen bonding, which ensured the modified BF/CS/n-HA composite membranes to possess excellent tensile strength, compared to the CS/n-HA and untreated BF/CS/n-HA composite membrane. Moreover, the weight ratio of the modified BF/CS/n-HA of 2:6:2 displayed the highest tensile strength, whose tensile strength was improved by over two folds than that of CS/n-HA composite membrane owing to the most suitable proportion. In addition, in vitro simulated body fluids soaking results indicated that the modified BF/CS/n-HA composite membranes presented different degradation rates and could promote the apatite to deposit by investigating the weight loss, water absorption rate, SEM morphology observation and tensile strength reduce. All results revealed that the ionic crosslinking of carboxylated BF and CS was conducive to fabricate BF/CS/n-HA composite membrane with higher mechanical property, more suitable degradation and better bioactivity, in contrast with the CS/n-HA and untreated BF/CS/n-HA composite membranes, which had a great promising to obtain more satisfactory GBR membrane, moreover, the study would provide a new application for natural BF in the biomedical membrane field.

Graphic abstract

In this manuscript, carboxylated bamboo fiber (BF) was designed to be introduced into the chitosan/nano-hydroxyapatite (CS/n-HA) system to fabricate a novel BF/CS/n-HA composite membranes with different weight ratios. The formation mechanism of the BF/CS/n-HA ternary composite membrane and the effect of modified BF different contents on the BF/CS/n-HA composite membranes were studied, and the main purpose of the study was to explore the feasibility of preparing the new BF/CS/n-HA composite membrane by ionic crosslinking. Results revealed that the ionic crosslinking of carboxylated BF and CS played an essential role in fabricating BF/CS/n-HA composite membrane with higher mechanical properties, more suitable degradation and better bioactivity. The study proved that the carboxylated BF/CS/n-HA composite membrane fabricated by ionic crosslinking had a great potential in obtaining more satisfactory GBR membrane in contrast with the CS/n-HA and untreated BF/CS/n-HA composite membranes, which would open up a new application for natural BF in the biomedical membrane field.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

References

  • Aryaei A, Liu J, Jayatissa AH, Jayasuriya AC (2015) Cross-linked chitosan improves the mechanical properties of calcium phosphate-chitosan cement. Mat Sci Eng C-Mater 54:14–19

    CAS  Google Scholar 

  • Baheti V, Militky J, Marsalkova M (2013) Mechanical properties of poly lactic acid composite films reinforced with wet milled jute nanofibers. Polym Compos 34:2133–2141

    CAS  Google Scholar 

  • Bierhalz ACK, Moraes AM (2017) Composite membranes of alginate and chitosan reinforced with cotton or linen fibers incorporating epidermal growth factor. Mat Sci Eng C-Mater 76:287–294

    CAS  Google Scholar 

  • Bottino MC, Thomas V, Schmidt G, Vohra YK, Chu TMG, Kowolik MJ, Janowski GM (2012) Recent advances in the development of GTR/GBR membranes for periodontal regeneration-A materials perspective. Dent Mater 8:703–721

    Google Scholar 

  • Cao LH, Weng WZ, Chen X, Ding YT, Yan YG, Li HH, Zhao H, Shin JW, Wei J, Ji F, Su JC (2015) Development of degradable and bioactive composite as bone implants by incorporation of mesoporous bioglass into poly(l-lactide). Composites B 77:454–461

    CAS  Google Scholar 

  • Dias LL, Mansur HS, Donnici CL, Pereira MM (2011) Synthesis and characterization of chitosan-polyvinyl alcohol-bioactive glass hybrid membranes. Biomatter 1:114–119

    PubMed  PubMed Central  Google Scholar 

  • Elgali I, Turri A, **a W, Norlindh B, Johansson A, Dahlin C, Thomsen P, Omar O (2016) Guided bone regeneration using resorbable membrane and different bone substitutes: early histological and molecular events. Acta Biomater 29:409–423

    CAS  Google Scholar 

  • Ezati M, Safavipour H, Houshmand B, Faghihi S (2018) Development of a PCL/gelatin/chitosan/beta-TCP electrospun composite for guided bone regeneration. Prog Biomater 7:225–237

    CAS  PubMed  PubMed Central  Google Scholar 

  • Grigoriadou I, Nianias N, Hoppe A, Terzopoulou Z, Bikiaris D, Will J, Hum J, Roether JA, Detsch R, Boccaccini AR (2014) Evaluation of silica-nanotubes and strontium hydroxyapatite nanorods as appropriate nanoadditives for poly(butylene succinate) biodegradable polyester for biomedical applications. Composites B 60:49–59

    CAS  Google Scholar 

  • Guan QQ, Chen JJ, Chen D, Chai XS, He L, Peng LC, Zhang JH, Li J (2019) A new sight on the catalytic oxidation kinetic behaviors of bamboo cellulose fibers under TEMPO-oxidized system: the fate of carboxyl groups in treated pulps. J Catal 370:304–309

    CAS  Google Scholar 

  • Guimaraes M, Botaro VR, Novack KM, Teixeira FG, Tonoli GHD (2015) Starch/PVA-based nanocomposites reinforced with bamboo nanofibrils. Ind Crops Prod 70:72–83

    CAS  Google Scholar 

  • Gurunathan T, Mohanty S, Nayak SK (2015) A review of the recent developments in biocomposites based on natural fibres and their application perspectives. Composites A 77:1–25

    CAS  Google Scholar 

  • Huang D, Niu LL, Li J, Du JJ, Wei Y, Hu YC, Lian XJ, Chen WY, Wang KQ (2018) Reinforced chitosan membranes by microspheres for guided bone regeneration. J Mech Behav Biomed 81:195–201

    CAS  Google Scholar 

  • Jiang LY, Li YB, Wang XJ, Zhang L, Wen JQ, Gong M (2008) Preparation and properties of nano-hydroxyapatite/chitosan/carboxymethy cellulose composite scaffold. Carbohyd Polym 74:680–684

    CAS  Google Scholar 

  • Jiang LY, Li YB, **ong CD (2009) A novel composite membrane of chitosan-carboxymethyl cellulose polyelectrolyte complex membrane filled with nano-hydroxyapatite I. Preparation and properties. J Mater Sci 16:65

    Google Scholar 

  • Jiang T, Carbone EJ, Lo KWH, Laurencin CT (2015) Electrospinning of polymer nanofibers for tissue regeneration. Prog Polym Sci 46:1–24

    Google Scholar 

  • Jiang LY, Li Y, **ong CD, Su SP, Ding HJ (2017) Preparation and properties of bamboo fiber/nano-hydroxyapatite/poly(lactic-co-glycolic) composite scaffold for bone tissue engineering. ACS Appl Mater Int 9:4890–4897

    CAS  Google Scholar 

  • Jiang LY, Ma BL, Li Y, Ding HJ, Su SP, **ong CD (2019) Effect of bamboo fiber on the degradation behavior and in vitro cytocompatibility of the nano-hydroxyapatite/poly(lactide-co-glycolide) (n-HA/PLGA) composite. Cellulose 26:1099–1110

    CAS  Google Scholar 

  • Khalil HPSA, Bhat IUH, Jawaid M, Zaidon A, Hermawan D, Hadi YS (2012) Bamboo fibre reinforced biocomposites: a review. Mater Des 42:353–368

    Google Scholar 

  • Kokubo T, Takadama H (2006) How useful is SBF in predicting in vivo bone bioactivity? Biomaterials 27:2907–2915

    CAS  PubMed  Google Scholar 

  • Kumar JP, Lakshmi L, Jyothsna V, Balaji DR, Saravanan S, Moorthi A, Selvamurugan N (2014) Synthesis and characterization of diopside particles and their suitability along with chitosan matrix for bone tissue engineering in vitro and in vivo. J Biomed Nanotechnol 10:970–981

    CAS  PubMed  Google Scholar 

  • Li WW, Ma GW, Brazile B, Li N, Dai W, Butler JR, Claude AA, Wertheim JA, Liao J, Wang B (2015) Investigating the potential of amnion-based scaffolds as a barrier membrane for guided bone regeneration. Langmuir 31:8642–8653

    CAS  PubMed  Google Scholar 

  • Li P, Jia Z, Wang Q, Tang Wang PM, Wang K, Fang J, Zhao C, Ren F, Ge X, Lu X (2018) A resilient and flexible chitosan/silk cryogel incorporated Ag/Sr co-doped nanoscale hydroxyapatite for osteoinductivity and antibacterial properties. J Mater Chem B 6:7427–7438

    CAS  PubMed  Google Scholar 

  • Liu D, Song J, Anderson DP, Chang PR, Hua Y (2012) Bamboo fiber and its reinforced composites: structure and properties. Cellulose 19:1449–1480

    CAS  Google Scholar 

  • Long HB, Wu ZQ, Dong QQ, Shen YT, Zhou WY, Luo Y, Zhang CQ, Dong XM (2019) Effect of polyethylene glycol on mechanical properties of bamboo fiber-reinforced polylactic acid composites. J Appl Polym Sci 136:47709

    Google Scholar 

  • Manchineella S, Thrivikraman G, Basu B, Govindaraju T (2016) Surface-functionalized silk fibroin films as a platform to guide neuron-like differentiation of human mesenchymal stem cells. ACS Appl Mater Interface 8:22849–22859

    CAS  Google Scholar 

  • Norowski PA, Fujiwara T, Clem WC, Adatrow PC, Eckstein EC, Haggard WO, Bumgardner JD (2015) Novel naturally crosslinked electrospun nanofibrous chitosan mats for guided bone regeneration membranes: material characterization and cytocompatibility. J Tissue Eng Regen M 9:577–583

    CAS  Google Scholar 

  • Palazzo B, Izzo D, Scalera F, Cancelli AN, Gervaso F (2014) Bio-hybrid scaffolds for bone tissue engineering: nano-hydroxyapatite/chitosan composites. Key Eng Mater 631:300–305

    Google Scholar 

  • Park SM, Kim HS (2017) Preparation of acetylated chitosan/carbonated hydroxyapatite composite barriers for guided bone regeneration. Macromol Res 25:158–164

    CAS  Google Scholar 

  • Phuong HAL, Ayob NAI, Blanford CF, Rawi NFM, Szekely G (2019) Nonwoven membrane supports from renewable resources: bamboo fiber reinforced poly(lactic acid) composites. ACS Sustain Chem Eng 7:11885–11893

    Google Scholar 

  • Soundhar A, Kandasamy J (2019) Mechanical, chemical and morphological analysis of crab shell/sisal natural fiber hybrid composites. J Nat Fibers. https://doi.org/10.1080/15440478.2019.1691127

    Article  Google Scholar 

  • Tamburaci S, Tihminlioglu F (2017) Diatomite reinforced chitosan composite membrane as potential scaffold for guided bone regeneration. Mat Sci Eng C-Mater 80:222–231

    CAS  Google Scholar 

  • Tu Y, Chen C, Li YB, Hou Y, Huang M, Zhang L (2017) Fabrication of nano-hydroxyapatite/chitosan membrane with asymmetric structure and its applications in guided bone regeneration. Bio-Med Mater Eng 28:223–233

    CAS  Google Scholar 

  • Wang ZL, Xu Y, Wang Y, Ito Y, Zhang PB, Chen XS (2016) Enhanced in vitro mineralization and in vivo osteogenesis of composite scaffolds through controlled surface grafting of L-lactic acid oligomer on nano-hydroxyapatite. Biomacromol 17:818–829

    CAS  Google Scholar 

  • Wu ZY, Tang TT, Guo H, Tang SC, Niu YF, Zhang J, Zhang WJ, Ma R, Su JC, Liu CS, Wei J (2014) In vitro degradability, bioactivity and cell responses to mesoporous magnesium silicate for the induction of bone regeneration. Colloid Surf B 120:38–46

    CAS  Google Scholar 

  • **ang WY, Li SB, Li K, Li N (2016) Preparation of bamboo fibers adsorbent modified by citric acid. Chin J Environ Eng 10:5542–5548

    Google Scholar 

  • Xu LJ, Jiang LY, **ong CD, Jiang LX (2014) Effect of different synthesis conditions on the microstructure, crystallinity and solubility of Mg-substituted hydroxyapatite nanopowder. Adv Powder Technol 25:1142–1146

    CAS  Google Scholar 

  • Ye HL, Zhu JJ, Deng D, ** SE, Li JD, Man Y (2019) Enhanced osteogenesis and angiogenesis by PCL/chitosan/Sr-doped calcium phosphate electrospun nanocomposite membrane for guided bone regeneration. J Biomater Sci-Polym E 30:1505–1522

    CAS  Google Scholar 

  • Zhang Y, Wu H, Qiu Y (2010) Morphology and properties of hybrid composites based on polypropylene/polylactic acid blend and bamboo fiber. Bioresour Technol 101:7944–7950

    CAS  Google Scholar 

  • Zhang Y, Zhang X, Shi B, Miron RJ (2013) Membranes for guided tissue and bone regeneration. Ann Oral Maxillofac Surg 1:10

    Google Scholar 

  • Zhou T, Liu X, Sui BY, Liu C, Mo XM, Sun J (2017a) Development of fish collagen/bioactive glass/chitosan composite nanofibers as a GTR/GBR membrane for inducing periodontal tissue regeneration. Biomed Mater 12:055004

    PubMed  Google Scholar 

  • Zhou YS, Dong Q, Yang HJ, Liu X, Yin XZ, Tao YZ, Bai ZK, Xu WL (2017b) Photocrosslinked maleilated chitosan/ methacrylated poly (vinyl alcohol) bicomponent nanofibrous scaffolds for use as potential wound dressings. Carbohyd Polym 168:220–226

    CAS  Google Scholar 

Download references

Acknowledgments

The authors would like to acknowledge the support of the Science and technology project of Changsha (kq1907132), Innovation Platform Open Found of Hunan Provincial Education Department (Grant No. 17K055), Opening Found of Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education of China), Hunan Normal University (No. KLCBTCMR201812) and Hunan Engineering Laboratory for Preparation Technology of Poly(vinyl alcohol) Fiber Material, Huaihua University (Grant No. HGY201812).

Author information

Authors and Affiliations

  1. National & Local Joint Engineering Laboratory for New Petro-Chemical Materials and Fine Utilization of Resources, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, People’s Republic of China

    Shuo Tang, Liuyun Jiang, Bingli Ma, Chunyan Tang, Yue Wen, Na Zhang, Yan Zhang & Shengpei Su

  2. Key Laboratory of Sustainable Resources Processing and Advanced Materials, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, People’s Republic of China

    Shuo Tang, Liuyun Jiang, Bingli Ma, Chunyan Tang, Yue Wen, Na Zhang, Yan Zhang & Shengpei Su

  3. Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, People’s Republic of China

    Shuo Tang, Liuyun Jiang, Bingli Ma, Chunyan Tang, Yue Wen, Na Zhang, Yan Zhang & Shengpei Su

Authors
  1. Shuo Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Liuyun Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Bingli Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chunyan Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yue Wen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Na Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yan Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Shengpei Su
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Liuyun Jiang.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Tang, S., Jiang, L., Ma, B. et al. Preparation and characterization of bamboo fiber/chitosan/nano-hydroxyapatite composite membrane by ionic crosslinking. Cellulose 27, 5089–5100 (2020). https://doi.org/10.1007/s10570-020-03145-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10570-020-03145-2

Keywords

Search

Navigation