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Enzymatic Hydrolysis of Waste Cooking Oil by Lipase Catalysis: Simplex Mixture Design Optimization

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Abstract

A growing generation of waste cooking oil (WCO) and its inappropriate disposal has led to the worsening of serious environmental problems. Thus, such a massive amount of WCO generated by household consumption and the food industry encourages the development of new technologies for reusing it to produce products of high economic value. The use of lipases in enzymatic hydrolysis of WCO allows employing the free fatty acids (FFA) and glycerol in the production of various bioproducts in oleochemical industries. Enzymatic hydrolysis offers advantages if compared to conventional processes of FFA production (Colgate-Emery), as it can operate under milder pressure and temperature conditions and high substrate specificity, resulting in high purity products and reduced by-product formation. Therefore, a simultaneous use of three different types of lipases in WCO hydrolysis was evaluated. For such a purpose, a simplex centroid mixture design (SMD) was used to model the influence of lipase composition on hydrolysis yield. Three commercial lipases were used: Burkholderia cepacia (LBC), Rhizopus oryzae (LRO) and porcine pancreas (LPP). Results reveal that high reaction yields of approximately 97% hydrolysis were achieved. SMD provided optimal conditions for lipase mixtures and higher hydrolysis yield, resulting in 15% LPP lipase and 85% LBC.

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Acknowledgements

The present work was supported the Coordination for the Improvement of Higher Education Personnel (CAPES)—Financing Code 001. The authors also thank the Fundação de Amparo à Pesquisa do Estado de Minas Gerais—FAPEMIG for granting the master's degree scholarship to Grazielle Pereira and CNPq for the financial support (Process Number 409346/2018-7).

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Authors and Affiliations

  1. Federal University of Alfenas, Alfenas, MG, Brazil

    Grazielle P. R. Souza, Thais B. A. Correia & Ernandes B. Pereira

  2. Engineering School of Lorena-University of São Paulo, Lorena, SP, 12602-810, Brazil

    Willian S. M. Reis, Eduardo H. Bredda & Patrícia C. M. Da Rós

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  1. Grazielle P. R. Souza
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Correspondence to Patrícia C. M. Da Rós.

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Souza, G.P.R., Correia, T.B.A., Reis, W.S.M. et al. Enzymatic Hydrolysis of Waste Cooking Oil by Lipase Catalysis: Simplex Mixture Design Optimization. Catal Lett 153, 689–697 (2023). https://doi.org/10.1007/s10562-022-04025-z

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