SpringerLink
Log in

Metabolic regulation of ammonia emission in different senescence phenotypes of Nicotiana tabacum

  • Brief Communication
  • Published:
Biologia Plantarum

Abstract

In order to reveal the character of ammonia emission in senescent tobacco (Nicotiana tabacum), the content of NH4 +, total nitrogen, and soluble protein, and the activities of nitrogen metabolism-related enzymes were measured in leaves of a quick-leaf-senescence phenotype ZY90 and a slow-leaf-senescence phenotype NC89. Compared with NC89, ZY90 had a higher NH4 + accumulation, a lower glutamine synthetase activity, and a significantly higher stomatal ammonia compensation point, and ammonia emission during 40 to 60 d after leaf emergence. During senescence, the quick-leafsenescence phenotype was characterized by nitrogen re-transfer by ammonia emmission, whereas the slow-leafsenescence phenotype by nitrogen re-assimilation. The ammonia emission was primarily regulated by glutamine synthetase activity, apoplastic pH, and NH4 + content.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Abbreviations

DAE:

days after leaf emergence

GDH:

glutamate dehydrogenase

GS:

glutamine synthetase

[H+]apo :

apoplastic H+content

[NH4 +]apo :

apoplastic NH4 +content

pHapo :

apoplastic pH

χs :

ammonia stomatal compensation point

References

  • Agüera, E., Cabello, P., Haba, P.D.L.: Induction of leaf senescence by low nitrogen nutrition in sunflower (Helianthus annuus) plants. — Physiol. Plant. 138: 256–267, 2010.

    Article  PubMed  Google Scholar 

  • Cruz, C., Bio, A.F.M., Domínguez-Valdivia, M.D., Aparicio-Tejo, P.M., Lamsfus, C., Martins-Loução, M.A.: How does glutamine synthetase activity determine plant tolerance to ammonium. — Planta 223: 1068–1080, 2006.

    Article  CAS  PubMed  Google Scholar 

  • Duan, W.J., Yang, T.Z., Dai, Y., LI, D.L., Zhang, X.Q., Liu, H.B., Wang, C.G.: Stomatal NH3 compensation point and its metabolic regulation in senescence phenotypes of Nicotiana tabacum. — Biol. Plant. 56: 771–774. 2012.

    Article  CAS  Google Scholar 

  • Herrmann, B., Mattsson, M., Jones, S.K., Cellier, P., Milford, C., Sutton, M.A., Schjoerring, J.K., Neftel, A.: Vertical structure and diurnal variability of ammonia exchange potential within an intensively managed grass canopy. — Biogeosciences 6: 15–23, 2009.

    Article  CAS  Google Scholar 

  • Horneck, D.A., Miller, R.O.: Determination of total nitrogen in plant tissue. — In: Karla, Y.P. (ed.): Handbook of Reference Methods for Plant Analysis. Pp. 75–83. CRC Press, New York 1998.

    Google Scholar 

  • Husted, S., Schjoerring, J.K.: Ammonia flux between oilseed rape plants and the atmosphere in response to changes in leaf temperature, light intensity and air humidity. — Plant Physiol. 112: 67–74, 1996.

    PubMed Central  CAS  PubMed  Google Scholar 

  • Husted, S., Hebbern, C.A., Mattsson, M., Schjoerring, J.K.: Determination of ammonium, low molecular weight amines and amides in plant tissue. — Physiol. Plant. 109: 167–179, 2000.

    Article  CAS  Google Scholar 

  • Husted, S., Schjoerring, J.K.: Apoplastic pH and ammonium concentration in leaves of Brassica napus L. — Plant Physiol. 109: 1453–1460, 1995.

    PubMed Central  CAS  PubMed  Google Scholar 

  • Masclaux, D., C., Valadier, M.H., Carrayol, E., Reisdorf-Cren, M. Hirel, B.: Diurnal changes in the expression of glutamate dehydrogenase and nitrate reductase are involved in the C/N balance of tobacco source leaves. — Plant Cell Environ. 25: 1451–1462, 2002.

    Article  Google Scholar 

  • Massad, R.S., Loubet, B., Tuzet, A., Cellier, P.: Relationship between ammonia stomatal compensation point and nitrogen metabolism in arable crops: current status of knowledge and potential modeling approaches. — Environ. Pollut. 154: 390–403, 2008.

    Article  CAS  PubMed  Google Scholar 

  • Mattsson, M., Schjoerring, J.K.: Senescence-induced changes in apoplastic and bulk tissue ammonia concentrations of ryegrass leaves. — New Phytol. 160: 489–499, 2003.

    Article  Google Scholar 

  • Mattsson, M., Husted, S., Schjoerring, J.K.: Influence of nitrogen nutrition and metabolism on ammonia volatilization in plants. — Nutr. Cycl. Agroecosyst. 51: 35–40, 1998.

    Article  CAS  Google Scholar 

  • O’Neal, D., Joy, K.D.: Glutamine synthetase of pea leaves. I. Purification, stabilisation and pH optima. — Arch. Biochem. Biophys. 159: 113–122, 1973.

    Article  PubMed  Google Scholar 

  • Schjoerring, J.K., Husted, S., Mäck, G., Mattsson, M.: The regulation of ammonium translocation in plants. — J. exp. Bot. 53: 883–890, 2002.

    Article  CAS  PubMed  Google Scholar 

  • Schjoerring, J.K., Husted, S.M.G., Nielsen, K., Finnemann, J. Matt, M.: Physiological regulation of plant-atmosphere ammonia exchange. — Plant Soil. 221: 95–101, 2000.

    Article  CAS  Google Scholar 

  • Schjoerring, J.K., Mattsson, M.: Quantification of ammonia exchange between agricultural cropland and the atmosphere: measurements over two complete growth cycles of oilseed rape, wheat, barley, and pea. — Plant Soil. 228: 105–115, 2001.

    Article  CAS  Google Scholar 

  • Sommer, S.G, Schjoerring, J.K, Denmead, O.T.: Ammonia emission from mineral fertilizers and fertilized crops. — Adv. Agron. 82: 557–622, 2004.

    Article  CAS  Google Scholar 

  • Sparks, J.P.: Ecological ramifications of the direct foliar uptake of nitrogen. — Oecologia 159: 1–13, 2009.

    Article  PubMed  Google Scholar 

  • Sutton, M.A., Schjoerring, J.K. Wyers, G.P.: Plant-atmosphere exchange of ammonia. — Phil. Trans. roy. Soc. A 351: 261–278, 1995.

    Article  CAS  Google Scholar 

  • Turano, F.J., Dashner, R., Upadhayaya, A., Caldwell, C.R.: Purification of mitochondrial glutamate dehydrogenase from dark-grown soybean seedlings. — Plant Physiol. 112: 1357–1364, 1996.

    PubMed Central  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Henan Agricultural University, Zhengzhou, 450002, P.R. China

    Y. J. Wu, T. Z. Yang, Y. Y. Song, X. Q. Zhang, S. X. Xu, G. Xue & X. X. **ng

Authors
  1. Y. J. Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. Z. Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Y. Y. Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. X. Q. Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. X. Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. G. Xue
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. X. X. **ng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to T. Z. Yang.

Additional information

Acknowledgements: This work was supported by grants from the China Tobacco Corporation (Grant No. Ts-01-2011003) and the Henan Tobacco Corporation (Grant No.HYKJZD201401).

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wu, Y.J., Yang, T.Z., Song, Y.Y. et al. Metabolic regulation of ammonia emission in different senescence phenotypes of Nicotiana tabacum . Biol Plant 60, 190–194 (2016). https://doi.org/10.1007/s10535-015-0556-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10535-015-0556-4

Additional key words

Search

Navigation