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Identification and sequence analysis of cDNA fragments relative to ovary development of Cymbidium hybridium after pollination

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Biologia Plantarum

Abstract

In this study, seven cDNA fragments of genes, differentially expressed in ovaries after pollination in Cymbidium hybridium, were identified and characterized by mRNA differential display reverse transcription polymerase chain reaction (DDRT-PCR). Four (CDD-313, CDD-272, CDD-265, CDD-243) among these seven cDNA fragments showed no significant homology with ESTs or genes in the databases of NCBI; another three (CDD-193, CDD-218, CDD-470) showed significant homologies with sequences encoding components of an ABC-type transporter, a GTPase and 40S ribosomal S3 proteins (RPS3), respectively. The differential expression patterns of them were confirmed by reverse Northern dot blot analysis. More interestingly, CDD-470 appeared to be present and highly expressed in the pollinated ovaries and encoded a new factor of RPS3 participating in cell growth and proliferation. We deduced that this 40s ribosomal S3 like protein was involved in ovary development of orchids.

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Abbreviations

BLAST:

basic local alignment search tool

DDRT-PCR:

differential display RT-PCR

PAGE:

polyacrylamide gel electrophoresis

PCR:

polymerase chain reaction

RPS3:

40s ribosomal S3 proteins

RT-PCR:

reverse transcription-PCR

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Authors and Affiliations

  1. College of Life Sciences, Nankai University, Tian**, 300071, China

    X. Q. Chen, C. G. Wang, Y. Zhang, W. Q. Song & R. Y. Chen

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  1. X. Q. Chen
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Correspondence to W. Q. Song.

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Chen, X.Q., Wang, C.G., Zhang, Y. et al. Identification and sequence analysis of cDNA fragments relative to ovary development of Cymbidium hybridium after pollination. Biol Plant 51, 249–256 (2007). https://doi.org/10.1007/s10535-007-0050-8

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