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Analysis of Methylome of Different Forms of Basal Cell Hyperplasia and Squamous Cell Metaplasia of Bronchial Epithelium

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Bulletin of Experimental Biology and Medicine Aims and scope

Squamous cell lung cancer (SCLC) occurs as a result of dysregenerative changes in the bronchial epithelium: basal cell hyperplasia (BCH), squamous cell metaplasia (SM), and dysplasia. We previously suggested that combinations of precancerous changes detected in the small bronchi of patients with SCLC may reflect various “scenarios” of the precancerous process: isolated BCH→stop** at the stage of hyperplasia, BCH+SM→progression of hyperplasia into metaplasia, SM+dysplasia→progression of metaplasia into dysplasia. In this study, DNA methylome of various forms of precancerous changes in the bronchial epithelium of SCLC patients was analyzed using the genome-wide bisulfite sequencing. In BCH combined with SM, in contrast to isolated BCH, differentially methylated regions were identified in genes of the pathogenetically significant MET signaling pathway (RNMT, HPN). Differentially methylated regions affecting genes involved in inflammation regulation (IL-23, IL-23R, IL12B, IL12RB1, and FIS1) were detected in SM combined with dysplasia in comparison with SM combined with BCH. The revealed changes in DNA methylation may underlie various “scenarios” of the precancerous process in the bronchial epithelium.

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Authors and Affiliations

  1. Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia

    A. A. Ponomaroyva, A. A. Schegoleva, P. A. Gervas, T. S. Gerashchenko, O. V. Pankova, V. M. Perelmuter, N. V. Cherdyntseva & E. V. Denisov

  2. Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia

    N. I. Ershov

Authors
  1. A. A. Ponomaroyva
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  2. A. A. Schegoleva
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  3. P. A. Gervas
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  4. T. S. Gerashchenko
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  5. O. V. Pankova
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  6. N. I. Ershov
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  7. V. M. Perelmuter
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  8. N. V. Cherdyntseva
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  9. E. V. Denisov
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Corresponding author

Correspondence to A. A. Ponomaroyva.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 177, No. 1, pp. 107-112, January, 2024

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Ponomaroyva, A.A., Schegoleva, A.A., Gervas, P.A. et al. Analysis of Methylome of Different Forms of Basal Cell Hyperplasia and Squamous Cell Metaplasia of Bronchial Epithelium. Bull Exp Biol Med (2024). https://doi.org/10.1007/s10517-024-06138-4

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