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Effect of Carnosine on the Activity of Matrix Metalloproteinase-2 and Oxidative Stress in the Kidneys in Experimental Urate Nephrolithiasis

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The effect of carnosine on MMP-2 activity and oxidative stress in the kidneys in experimental urate nephrolithiasis was studied. Urate nephrolithiasis was modeled in Wistar rats by intragastric administration of a mixture of oxonic and uric acids. Carnosine was administered intragastrically through a tube in a dose of 15 mg/kg. In rats treated with carnosine, the concentration of MMP-2 in the urine decreased by 3.7 times, and the excretion of MMP-2 with urine decreased by 4.3 times. In the homogenate of the kidneys from rats treated with carnosine, the concentration of TBA-reactive substances decreased by 5 times and the concentration of MMP-2 decreased by 12.7%. After treatment with carnosine, the number of histologically confirmed cases of urate nephrolithiasis decreased by 2 times, while the mean size of urate deposits decreased by 2.7 times. Thus, carnosine inhibits MMP-2 and reduces the intensity of oxidative stress in the kidneys, which prevents the development of urate nephrolithiasis.

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References

  1. Covington MD, Bayless KJ, Burghardt RC, Davis GE, Parrish AR. Ischemia-induced cleavage of cadherins in NRK cells: evidence for a role of metalloproteinases. Am. J. Physiol. Renal. Physiol. 2005;289(2):F280-288. https://doi.org/10.1152/ajprenal.00351.2004

    Article  CAS  PubMed  Google Scholar 

  2. Chang HR, Yang SF, Li ML, Lin CC, Hsieh YS, Lian JD. Relationships between circulating matrix metalloproteinase-2 and -9 and renal function in patients with chronic kidney disease. Clin. Chim. Acta. 2006;366(1-2):243-248. https://doi.org/10.1016/j.cca.2005.10.007

    Article  CAS  PubMed  Google Scholar 

  3. Tashiro K, Koyanagi I, Ohara I, Ito T, Saitoh A, Horikoshi S, Tomino Y. Levels of urinary matrix metalloproteinase-9 (MMP-9) and renal injuries in patients with type 2 diabetic nephropathy. J. Clin. Lab. Anal. 2004;18(3):206-210. https://doi.org/10.1002/jcla.20024

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Wigner P, Grębowski R, Bijak M, Szemraj J, Saluk-Bijak J. The Molecular Aspect of Nephrolithiasis Development. Cells. 2021;10(8):1926. https://doi.org/10.3390/cells10081926

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Su HY, Yang C, Liang D, Liu HF. Research Advances in the Mechanisms of Hyperuricemia-Induced Renal Injury. Biomed. Res. Int. 2020;2020:5817348. https://doi.org/10.1155/2020/5817348

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Catania JM, Chen G, Parrish AR. Role of matrix metalloproteinases in renal pathophysiologies. Am. J. Physiol. Renal. Physiol. 2007;292(3):F905-F911. https://doi.org/10.1152/ajprenal.00421.2006

    Article  CAS  PubMed  Google Scholar 

  7. Ali MM, Mahmoud AM, Le Master E, Levitan I, Phillips SA. Role of matrix metalloproteinases and histone deacetylase in oxidative stress-induced degradation of the endothelial glycocalyx. Am. J. Physiol. Heart Circ. Physiol. 2019;316(3):H647-H663. https://doi.org/10.1152/ajpheart.00090.2018

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Perfil’ev VYu, Zverev YaF, Zharikov AYu, Bryukhanov VM. The role of free radical oxidation in the development of experimental urate nephropathy. Bull. Exp. Biol. Med. 2017;163(1):28-30. https://doi.org/10.1007/s10517-017-3730-1

  9. Kim EH, Kim ES, Shin D, Kim D, Choi S, Shin YJ, Kim KA, Noh D, Caglayan AB, Rajanikant GK, Majid A, Bae ON. Carnosine Protects against Cerebral Ischemic Injury by Inhibiting Matrix-Metalloproteinases. Int. J. Mol. Sci. 2021;22(14):7495. https://doi.org/10.3390/ijms22147495

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Ghodsi R, Kheirouri S. Carnosine and advanced glycation end products: a systematic review. Amino Acids. 2018;50(9):1177-1186. https://doi.org/10.1007/s00726-018-2592-9

    Article  CAS  PubMed  Google Scholar 

  11. Perfilev VYu, Zverev YaF, Zharikov AYu. Conditions of urate nephrolithiasis and approaches to its modeling. Nefrologiya. 2017;21(4):48-54. Russian. https://doi.org/10.24884/1561-6274-2017-21-4-48-54

  12. Methods of Preclinical (Experimental) Study of the Effect of Drugs on Renal Function). Novosibirsk, 2013. Russian.

  13. Khafaziyanova RKh, Burykin IM, Aleeva GN. Mathematical Statistics in Experimental and Clinical Pharmacology. Kazan, 2006. Russian.

  14. Baran EJ. Metal complexes of carnosine. Biochemistry (Mosc). 2000;65(7):789-797.

    CAS  PubMed  Google Scholar 

  15. Wozniak J, Floege J, Ostendorf T, Ludwig A. Key metalloproteinase-mediated pathways in the kidney. Nat. Rev. Nephrol. 2021;17(8):513-527. https://doi.org/10.1038/s41581-021-00415-5

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Altai State Medical University, Ministry of Health of the Russian Federation, Barnaul, Russia

    A. Yu Zharikov, A. S. Kalnitsky, O. N. Mazko, O. G. Makarova & I. P. Bobrov

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  1. A. Yu Zharikov
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  2. A. S. Kalnitsky
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  3. O. N. Mazko
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  4. O. G. Makarova
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  5. I. P. Bobrov
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Correspondence to A. S. Kalnitsky.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 174, No. 9, pp. 311-314, September, 2022

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Zharikov, A.Y., Kalnitsky, A.S., Mazko, O.N. et al. Effect of Carnosine on the Activity of Matrix Metalloproteinase-2 and Oxidative Stress in the Kidneys in Experimental Urate Nephrolithiasis. Bull Exp Biol Med 174, 326–329 (2023). https://doi.org/10.1007/s10517-023-05701-9

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  • DOI: https://doi.org/10.1007/s10517-023-05701-9

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