Abstract
To select a new strain of Neoporphyra haitanensis with thin blades and high-temperature resistance, an intraspecific hybridization of a cultivated strain (WT-bo, ♂) and improved strain (HR-5, ♀) in N. haitanensis was carried out, and then a new strain qF-15 was isolated from the offspring based on heat stress and characteristics analysis. After being cultured for 40 days, the mean thickness of the blades was 22.3 μm in qF-15, which was 9.7% and 8.3% lower than that of WT-bo and HR-5, respectively; the total phycobiliprotein content was 122.9 mg/g in qF-15, which was 41.6% and 5.9% higher than that of WT-bo and HR-5, respectively. After being cultured for 45 days, the mean length of the blades was 70.4 cm in qF-15, which was 2.6- and 1.6-fold of that of WT-bo and HR-5, respectively. After being cultured for 7 days at 30 °C, the survival rate of conchospore germlings was 25.9% in qF-15, which was 439.6% and 223.8% higher than that of WT-bo and HR-5, respectively. The blades were cultured for 25 days at 23 °C and then cultured for another 20 days at 30 °C. Plenty of cells died in the blades of WT-bo, while only a little of cells died in the blades of HR-5 and qF-15. After the conchocelis-inoculated shells experienced the process of high temperature and shrunk of light, the time for the emergence of a lot of conchosporangial branch was about 20 days for the three strains. In summary, qF-15 was characterized by thin blades, high-temperature resistance of conchospore germlings and blades, fast growth, high phycobiliprotein content, and fast differentiation rate of conchocelis-inoculated shell, and it is expected to be applied in production and cultivation.
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Data Availability
The data that support the findings of this study are available from the corresponding author upon reasonable request.
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The research was supported by the Public Welfare Science and Technology Projects of Ningbo (2022S160), Youth Fund Project of National Natural Science Foundation of China (32102773), and Shanghai Sailing Program (21YF1417100).
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Fei-Fei Lu performed the experiments, analyzed the data, and drafted the manuscript. Hong-Chang Ding and **ng-Hong Yan participated in the design of this study. All authors have read and approved the final manuscript.
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Lu, FF., Ding, HC. & Yan, XH. Selection and characterization of a new strain with thin blades and high-temperature resistance in Neoporphyra haitanensis. Aquacult Int 32, 447–460 (2024). https://doi.org/10.1007/s10499-023-01170-z
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DOI: https://doi.org/10.1007/s10499-023-01170-z