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Pelagicola litorisediminis sp. nov., a novel alphaproteobacterium isolated from tidal flat sediment

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Abstract

A Gram-negative, aerobic, non-motile and rod-shaped or ovoid bacterial strain, designated D1-W8T, was isolated from a tidal flat on the South Sea in South Korea. Strain D1-W8T was found to grow optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain D1-W8T clustered with the type strain of Pelagicola litoralis showing 97.1 % sequence identity. 16S rRNA gene sequences of the type strains of other species exhibited lower similarity values. Strain D1-W8T was determined to contain Q-10 as the predominant ubiquinone and C18:1 ω7c as the predominant fatty acid. The major polar lipids of strain D1-W8T were identified as phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain D1-W8T was determined to be 57.9 mol% and its DNA–DNA relatedness value with the type strain of P. litoralis was 17 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain D1-W8T is separate from P. litoralis. On the basis of the data presented, strain D1-W8T is considered to represent a novel species of the genus Pelagicola, for which the name Pelagicola litorisediminis sp. nov. is proposed. The type strain is D1-W8T (= KCTC 32327T = CECT 8287T).

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Acknowledgments

This study was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) and the Program for Collection, Management and Utilization of Biological Resources and BK21 program from the Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea.

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Authors and Affiliations

  1. Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea

    Sooyeon Park, Yong-Taek Jung & Jung-Hoon Yoon

  2. University of Science and Technology (UST), 113 Gwahangno, Yuseong, Daejeon, South Korea

    Yong-Taek Jung

Authors
  1. Sooyeon Park
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  2. Yong-Taek Jung
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  3. Jung-Hoon Yoon
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Correspondence to Jung-Hoon Yoon.

Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain D1-W8T is KC708867.

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Park, S., Jung, YT. & Yoon, JH. Pelagicola litorisediminis sp. nov., a novel alphaproteobacterium isolated from tidal flat sediment. Antonie van Leeuwenhoek 104, 103–110 (2013). https://doi.org/10.1007/s10482-013-9930-4

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  • DOI: https://doi.org/10.1007/s10482-013-9930-4

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