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Distribution characteristics of ammonia-oxidizing microorganisms and their responses to external nitrogen and carbon in sediments of a freshwater reservoir, China

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Abstract

Ammonia oxidation driven by ammonia-oxidizing archaea (AOA) and bacteria (AOB) plays a significant role in the nitrogen cycle, but the mechanism of this important action in reservoir sediments is unclear. In this study, based on quantitative PCR and Illumina high-throughput sequencing of the ammonia monooxygenase (AMO) gene, the abundance, diversity and community structure of AOA and AOB in Miyun Reservoir sediments were investigated. The key environmental factors for regulating community structure, as well as the response of AOA and AOB abundance and transcriptional activity to external ammonia and organic carbon, were also explored. The results showed that the diversity and abundance of AOB in the reservoir sediments were higher than those of AOA, and the community structure of AOA showed a different spatial distribution characteristics compared with those of AOB. AOA community structure was closely related to ammonia nitrogen concentration and water depth, while that of AOB were not significantly affected by environmental factors. AOB were more sensitive to concentration variations of ammonia nitrogen and organic carbon from exogenous sources. Therefore, in Miyun Reservoir sediments, AOA were more sensitive to the influence of environmental factors in the reservoir area, while AOB were more sensitive to the influence of exogenous sources.

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References

  • Besaury L, Ghiglione JF, Quillet L (2014) Abundance, activity, and diversity of archaeal and bacterial communities in both uncontaminated and highly copper-contaminated marine sediments. Mar Biotechnol (NY) 16:230–242

    Article  CAS  Google Scholar 

  • Bollmann A, Bullerjahn GS, McKay RM (2014) Abundance and diversity of ammonia-oxidizing archaea and bacteria in sediments of trophic end members of the Laurentian Great Lakes, Erie and Superior. PLoS ONE 9:e97068

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Borcard D, Gillet F, Legendre P (2011) Canonical ordination. Numerical ecology with R. Springer, New York, pp 153–225

    Chapter  Google Scholar 

  • Brochier-Armanet C, Boussau B, Gribaldo S, Forterre P (2008) Mesophilic Crenarchaeota: proposal for a third archaeal phylum, the Thaumarchaeota. Nat Rev Microbiol 6:245–252

    Article  CAS  PubMed  Google Scholar 

  • Burford MA, Green SA, Cook AJ, Johnson SA, Kerr JG, O’Brien KR (2012) Sources and fate of nutrients in a subtropical reservoir. Aquat Sci 74:179–190

    Article  CAS  Google Scholar 

  • Cao H, Li M, Hong Y, Gu JD (2011) Diversity and abundance of ammonia-oxidizing archaea and bacteria in polluted mangrove sediment. Syst Appl Microbiol 34:513–523

    Article  CAS  PubMed  Google Scholar 

  • Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N et al (2010) QIIME allows analysis of high-throughput community sequencing data. Nat Methods 7:335–336

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Chen Y, Liu Y, Wang X (2017) Spatiotemporal variation of bacterial and archaeal communities in sediments of a drinking reservoir, Bei**g, China. Appl Microbiol Biotechnol 101:3379–3391

    Article  CAS  PubMed  Google Scholar 

  • Chen S, Gao R, **ang X, Yang H, Ma H, Zheng T, **ao Y et al (2021) Straw mulching and nitrogen application altered ammonia oxidizers communities and improved soil quality in the alkaline purple soil of southwest China. AMB Express 11:52

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Cheng W, Zhang J, Wang Z, Wang M, **e S (2013) Bacterial communities in sediments of a drinking water reservoir. Ann Microbiol 64:875–878

    Article  CAS  Google Scholar 

  • Daebeler A, Herbold C, Vierheilig J, Sedlacek CJ, Pjevac P, Albersten M, Kirkegaard RH, Torre J, Daims H, Wagner M (2017) Cultivation and genomic analysis of candidatus nitrosocaldus islandicus, a novel obligately thermophilic ammonia-oxidizing Thaumarchaeon

  • Dai X, Guo Q, Song D, Zhou W, Liu G, Liang G, He P, Sun G, Yuan F, Liu Z (2021) Long-term mineral fertilizer substitution by organic fertilizer and the effect on the abundance and community structure of ammonia-oxidizing archaea and bacteria in paddy soil of south China. Eur J Soil Biol 103:103288

    Article  CAS  Google Scholar 

  • Dang C, Liu W, Lin Y, Zheng M, Jiang H, Chen Q, Ni J (2018) Dominant role of ammonia-oxidizing bacteria in nitrification due to ammonia accumulation in sediments of Danjiangkou reservoir, China. Appl Microbiol Biotechnol 102:3399–3410

    Article  CAS  PubMed  Google Scholar 

  • Deh A, Yan DB, Jas C (2019) Nitrogen cycling during wastewater treatment - sciencedirect. Adv Appl Microbiol 106:113–192

    Article  CAS  Google Scholar 

  • Dhami KK, Joseph L, Roshier DA, Heinsohn R, Peters JL (2013) Multilocus phylogeography of Australian teals (Anasspp.): a case study of the relationship between vagility and genetic structure. J Avian Biol 44:169–178

    Article  Google Scholar 

  • Edgar RC (2013) UPARSE: highly accurate OTU sequences from microbial amplicon reads. Nat Methods 10:996–998

    Article  CAS  PubMed  Google Scholar 

  • Edgar RC, Haas BJ, Clemente JC, Quince C, Knight R (2011) UCHIME improves sensitivity and speed of chimera detection. Bioinformatics 27:2194–2200

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Fish JA, Chai B, Wang Q, Sun Y, Brown CT, Tiedje JM, Cole JR (2013) FunGene: the functional gene pipeline and repository. Front Microbiol 4:291

    Article  PubMed  PubMed Central  Google Scholar 

  • Francis CA, Roberts KJ, Beman JM, Santoro AE, Oakley BB (2005) Ubiquity and diversity of ammonia-oxidizing archaea in water columns and sediments of the ocean. Proc Natl Acad Sci USA 102:14683–14688

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Funes A, de Vicente J, Cruz-Pizarro L, Alvarez-Manzaneda I, de Vicente I (2016) Magnetic microparticles as a new tool for lake restoration: a microcosm experiment for evaluating the impact on phosphorus fluxes and sedimentary phosphorus pools. Water Res 89:366–374

    Article  CAS  PubMed  Google Scholar 

  • Gao JF, Luo X, Wu GX, Li T, Peng YZ (2013) Quantitative analyses of the composition and abundance of ammonia-oxidizing archaea and ammonia-oxidizing bacteria in eight full-scale biological wastewater treatment plants. Bioresour Technol 138:285–296

    Article  CAS  PubMed  Google Scholar 

  • Gao S, Zhou G, Rees RM, Cao W (2020) Green manuring inhibits nitrification in a typical paddy soil by changing the contributions of ammonia-oxidizing archaea and bacteria. Appl Soil Ecol 156:103698

    Article  Google Scholar 

  • Hatzenpichler R, Lebedeva EV, Spieck E, Stoecker K, Richter A, Daims H, Wagner M (2008) A moderately thermophilic ammonia-oxidizing crenarchaeote from a hot spring. Proc Natl Acad Sci 105:2134–2139

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • He H, Zhen Y, Mi T, Fu L, Yu Z (2018) Ammonia-oxidizing archaea and bacteria differentially contribute to ammonia oxidation in sediments from adjacent waters of rushan bayn China. Front Microbiol 9:116

    Article  PubMed  PubMed Central  Google Scholar 

  • Jiang H, Dong H, Yu B, Lv G, Deng S, Berzins N, Dai M (2009) Diversity and abundance of ammonia-oxidizing archaea and bacteria in Qinghai Lake, Northwestern China. Geomicrobiol J 26:199–211

    Article  CAS  Google Scholar 

  • Junier P, Molina V, Dorador C, Hadas O, Kim OS, Junier T, Witzel JP, Imhoff JF (2010) Phylogenetic and functional marker genes to study ammonia-oxidizing microorganisms (AOM) in the environment. Appl Microbiol Biotechnol 85:425–440

    Article  CAS  PubMed  Google Scholar 

  • Kowalchuk GA, Stephen JR (2001) Ammonia-oxidizing bacteria: a model for molecular microbial ecology. Ann Rev Microbiol 55(1):485–529

    Article  CAS  Google Scholar 

  • Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li S, Gang D, Zhao S, Qi W, Liu H (2021) Response of ammonia oxidation activities to water-level fluctuations in riparian zones in a column experiment. Chemosphere 269:128702

    Article  CAS  PubMed  Google Scholar 

  • Liang D, Ouyang Y, Tiemann L, Robertson GP (2020) Niche differentiation of bacterial versus archaeal soil nitrifiers induced by ammonium inhibition along a management gradient. Front Microbiol 11:568588

    Article  PubMed  PubMed Central  Google Scholar 

  • Limpiyakorn T, Furhacker M, Haberl R, Chodanon T, Srithep P, Sonthiphand P (2013) amoA-encoding archaea in wastewater treatment plants: a review. Appl Microbiol Biotechnol 97:1425–1439

    Article  CAS  PubMed  Google Scholar 

  • Liu T-T, Yang H (2021a) An RNA-based study of the distribution of ammonia-oxidizing microorganisms in vertical sediment. Ecol Indic 121:107143

    Article  CAS  Google Scholar 

  • Liu TT, Yang H (2021b) Different nutrient levels, rather than seasonal changes, significantly affected the spatiotemporal dynamic changes of ammonia-oxidizing microorganisms in Lake Taihu. World J Microbiol Biotechnol 37:91

    Article  CAS  PubMed  Google Scholar 

  • Liu Y, Zhang J, Zhang X, **e S (2014) Depth-related changes of sediment ammonia-oxidizing microorganisms in a high-altitude freshwater wetland. Appl Microbiol Biotechnol 98:5697–5707

    Article  CAS  PubMed  Google Scholar 

  • Liu S, Hu B, He Z, Zhang B, Tian G, Zheng P, Fang F (2015) Ammonia-oxidizing archaea have better adaptability in oxygenated/hypoxic alternant conditions compared to ammonia-oxidizing bacteria. Appl Microbiol Biotechnol 99:8587–8596

    Article  CAS  PubMed  Google Scholar 

  • Liu J, Yu Z, Yao Q, Sui Y, Shi Y, Chu H, Tang C et al (2018a) Ammonia-oxidizing archaea show more distinct biogeographic distribution patterns than ammonia-oxidizing bacteria across the black soil zone of Northeast China. Front Microbiol 9:171

    Article  PubMed  PubMed Central  Google Scholar 

  • Liu Y, Liu J, Yao P, Ge T, Qiao Y, Zhao M, Zhang XH (2018b) Distribution patterns of ammonia-oxidizing archaea and bacteria in sediments of the eastern China marginal seas. Syst Appl Microbiol 41:658–668

    Article  CAS  PubMed  Google Scholar 

  • Liu L, Li S, Han J, Lin W, Luo J (2019) A Two-Step Strategy for the rapid enrichment of nitrosocosmicus-like ammonia-oxidizing thaumarchaea. Front Microbiol 10:875

    Article  PubMed  PubMed Central  Google Scholar 

  • Liu X, Wu Y, Sun R, Hu S, Mi X (2020) NH4+-N/NO3–N ratio controlling nitrogen transformation accompanied with NO2–N accumulation in the oxic-anoxic transition zone. Environ Res 189:109962

    Article  CAS  PubMed  Google Scholar 

  • Liu X, Hu S, Sun R, Wu Y, Qiao Z, Wang S, Zhang Z, Cui C (2021) Dissolved oxygen disturbs nitrate transformation by modifying microbial community, co-occurrence networks, and functional genes during aerobic-anoxic transition. Sci Total Environ 790:148245

    Article  CAS  PubMed  Google Scholar 

  • Liu X, Sun R, Hu S, Zhong Y, Wu Y (2022) Aromatic compounds releases aroused by sediment resuspension alter nitrate transformation rates and pathways during aerobic-anoxic transition. J Hazard Mater 424:127365

    Article  CAS  PubMed  Google Scholar 

  • Lymperopoulou DS, Kormas KA, Karagouni AD (2012) Variability of prokaryotic community structure in a drinking water reservoir (Marathonas, Greece). Microbes Environ 27:1–8

    Article  PubMed  Google Scholar 

  • Martens-Habbena W, Stahl DA (2011) Nitrogen metabolism and kinetics of ammonia-oxidizing archaea. Methods Enzymol 496:465–487

    Article  CAS  PubMed  Google Scholar 

  • Martens-Habbena W, Berube PM, Urakawa H, de la Torre JR, Stahl DA (2009) Ammonia oxidation kinetics determine niche separation of nitrifying Archaea and Bacteria. Nature 461:976–979

    Article  CAS  PubMed  Google Scholar 

  • McDonald D, Vázquez-Baeza Y, Koslicki D, McClelland J, Reeve N, Xu Z, Gonzalez A, Knight R (2018) Striped UniFrac: enabling microbiome analysis at unprecedented scale. Nat Methods 15:847–848

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Muema EK, Cadisch G, Röhl C, Vanlauwe B, Rasche F (2015) Response of ammonia-oxidizing bacteria and archaea to biochemical quality of organic inputs combined with mineral nitrogen fertilizer in an arable soil. Appl Soil Ecol 95:128–139

    Article  Google Scholar 

  • O’Sullivan CA, Wakelin SA, Fillery IRP, Roper MM (2013) Factors affecting ammonia-oxidising microorganisms and potential nitrification rates in southern Australian agricultural soils. Soil Res 51:240–252

    Article  CAS  Google Scholar 

  • Ouyang Y, Norton JM, Stark JM, Reeve JR, Habteselassie MY (2016) Ammonia-oxidizing bacteria are more responsive than archaea to nitrogen source in an agricultural soil. Soil Biol Biochem 96:4–15

    Article  CAS  Google Scholar 

  • Pester M, Rattei T, Flechl S, Grongroft A, Richter A, Overmann J, Reinhold-Hurek B, Loy A, Wagner M (2012) amoA-based consensus phylogeny of ammonia-oxidizing archaea and deep sequencing of amoA genes from soils of four different geographic regions. Environ Microbiol 14:525–539

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Prosser JI, Nicol GW (2010) Relative contributions of archaea and bacteria to aerobic ammonia oxidation in the environment. Environ Microbiol 10:2931–2941

    Article  CAS  Google Scholar 

  • Prosser JI, Nicol GW (2012) Archaeal and bacterial ammonia-oxidisers in soil: the quest for niche specialisation and differentiation. Trends Microbiol 20:523–531

    Article  CAS  PubMed  Google Scholar 

  • Purkhold U, Pommerening-Röser A, Juretschko S, Schmid MC, Koops H-P, Wagner M (2000) Phylogeny of all recognized species of ammonia oxidizers based on comparative 16S rRNA and amoa sequence analysis: implications for molecular diversity surveys. Appl Environ Microbiol 66:5368–5382

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Qiao Z, Sun R, Wu Y, Hu S, Mi X (2020) Characteristics and metabolic pathway of sediment biomass for heterotrophic nitrification and aerobic denitrification in aquatic ecosystems. Environ Res 191:110069

    Article  CAS  PubMed  Google Scholar 

  • Rotthauwe JH, Witzel KP, Liesack W (1997) The ammonia monooxygenase structural gene amoA as a functional marker: molecular fine-scale analysis of natural ammonia-oxidizing populations. Appl Environ Microb 63:4704-4712

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Shen JP, Zhang LM, Di HJ, He JZ (2012) A review of ammonia-oxidizing bacteria and archaea in Chinese soils. Front Microbiol 3:296

    CAS  PubMed  PubMed Central  Google Scholar 

  • Su Y, Wang W, Wu D, Huang W, Wang M, Zhu G (2018) Stimulating ammonia oxidizing bacteria (AOB) activity drives the ammonium oxidation rate in a constructed wetland (CW). Sci Total Environ 624:87–95

    Article  CAS  PubMed  Google Scholar 

  • Tao R, Wakelin SA, Liang Y, Chu G (2017) Response of ammonia-oxidizing archaea and bacteria in calcareous soil to mineral and organic fertilizer application and their relative contribution to nitrification. Soil Biol Biochem 114:20–30

    Article  CAS  Google Scholar 

  • Taylor AE, Zeglin LH, Wanzek TA, Myrold DD, Bottomley PJ (2012) Dynamics of ammonia-oxidizing archaea and bacteria populations and contributions to soil nitrification potentials. ISME J 6:2024–2032

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tourna M, Stieglmeier M, Spang A, Konneke M, Schintlmeister A, Urich T, Engel M et al (2011) Nitrososphaera viennensis, an ammonia oxidizing archaeon from soil. Proc Natl Acad Sci USA 108:8420–8425

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Trivedi C, Reich PB, Maestre FT, Hu HW, Singh BK, Delgado-Baquerizo M (2019) Plant-driven niche differentiation of ammonia-oxidizing bacteria and archaea in global drylands. ISME J 13:2727–2736

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wang X, Wang C, Bao L, **e S (2014) Impact of carbon source amendment on ammonia-oxidizing microorganisms in reservoir riparian soil. Ann Microbiol 65:1411–1418

    Article  CAS  Google Scholar 

  • Wang J, Kan J, Zhang X, **a Z, Zhang X, Qian G, Miao Y, Leng X, Sun J (2017) Archaea dominate the ammonia-oxidizing community in deep-sea sediments of the eastern Indian Ocean-from the equator to the Bay of Bengal. Front Microbiol 8:415

    PubMed  PubMed Central  Google Scholar 

  • Wang W, Liu W, Wu D, Wang X, Zhu G (2018) Differentiation of nitrogen and microbial community in the littoral and limnetic sediments of a large shallow eutrophic lake (Chaohu Lake, China). J Soils Sediments 19:1005–1016

    Article  CAS  Google Scholar 

  • Wang W, Liu W, Wang S, Wang M, Long X-E, Zhu G (2019) Abundance, contribution, and possible driver of ammonia-oxidizing archaea (AOA) in various types of aquatic ecosystems. J Soils Sediments 19:2114–2125

    Article  CAS  Google Scholar 

  • **a W, Zhang C, Zeng X, Feng Y, Weng J, Lin X, Zhu J et al (2011) Autotrophic growth of nitrifying community in an agricultural soil. ISME J 5:1226–1236

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • **ao R, Ran W, Hu S, Guo H (2021) The response of ammonia oxidizing archaea and bacteria in relation to heterotrophs under different carbon and nitrogen amendments in two agricultural soils. Appl Soil Ecol 158:103812

    Article  Google Scholar 

  • Xu Y-g, Yu W-t, Ma Q, Zhou H (2012) Responses of bacterial and archaeal ammonia oxidisers of an acidic luvisols soil to different nitrogen fertilization rates after 9 years. Biol Fertil Soils 48:827–837

    Article  CAS  Google Scholar 

  • Yang Y, Zhang J, Zhao Q, Zhou Q, Li N, Wang Y, **e S, Liu Y (2016) Sediment ammonia-oxidizing microorganisms in two plateau freshwater lakes at different trophic states. Microb Ecol 71:257–265

    Article  CAS  PubMed  Google Scholar 

  • Yao X, Zhang Y, Zhang L, Zhou Y (2018) A bibliometric review of nitrogen research in eutrophic lakes and reservoirs. J Environ Sci 66:274–285 (China)

    Article  CAS  Google Scholar 

  • Ying J, Li X, Wang N, Lan Z, He J, Bai Y (2017) Contrasting effects of nitrogen forms and soil pH on ammonia oxidizing microorganisms and their responses to long-term nitrogen fertilization in a typical steppe ecosystem. Soil Biol Biochem 107:10–18

    Article  CAS  Google Scholar 

  • Yu S, Yao P, Liu J, Zhao B, Zhang G, Zhao M, Yu Z, Zhang XH (2016) Diversity, abundance, and niche differentiation of ammonia-oxidizing prokaryotes in mud deposits of the Eastern China Marginal Seas. Front Microbiol 7:137

    PubMed  PubMed Central  Google Scholar 

  • Zeng Q, Qin L, Bao L, Li Y, Li X (2016) Critical nutrient thresholds needed to control eutrophication and synergistic interactions between phosphorus and different nitrogen sources. Environ Sci Pollut Res Int 23:21008–21019

    Article  CAS  PubMed  Google Scholar 

  • Zhang LM, Duff AM, Smith CJ (2018) Community and functional shifts in ammonia oxidizers across terrestrial and marine (soil/sediment) boundaries in two coastal Bay ecosystems. Environ Microbiol 20:2834–2853

    Article  CAS  PubMed  Google Scholar 

  • Zhao D, Zeng J, Wan W, Liang H, Huang R, Wu QL (2013) Vertical distribution of ammonia-oxidizing archaea and bacteria in sediments of a eutrophic lake. Curr Microbiol 67:327–332

    Article  CAS  PubMed  Google Scholar 

  • Zhao DY, Luo J, Zeng J, Wang M, Yan WM, Huang R, Wu QL (2014) Effects of submerged macrophytes on the abundance and community composition of ammonia-oxidizing prokaryotes in a eutrophic lake. Environ Sci Pollut Res Int 21:389–398

    Article  PubMed  Google Scholar 

  • Zheng L, Zhao X, Zhu G, Yang W, **a C, Xu T (2017) Occurrence and abundance of ammonia-oxidizing archaea and bacteria from the surface to below the water table, in deep soil, and their contributions to nitrification. MicrobiologyOpen 6:e00488

    Article  PubMed Central  CAS  Google Scholar 

  • Zhou W, Jiang X, Ouyang J, Lu B, Liu W, Liu G (2020) Environmental factors, more than spatial distance, explain community structure of soil ammonia-oxidizers in wetlands on the qinghai-tibetan plateau. Microorganisms 8:933

    Article  CAS  PubMed Central  Google Scholar 

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Acknowledgements

Thanks are due to non-point source team for assistance with the sample collection and to Zhiwei Yang, Shuguang **e for valuable discussion.

Funding

This work was supported by the Bei**g Natural Science Fund-Bei**g Municipal Education Commission jointly funded key projects (KZ201810028047), and the National Natural Science Foundation of China (41271495).

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Authors and Affiliations

  1. College of Resources Environment and Tourism, Capital Normal University, Bei**g, 100048, China

    **gyu Huang, **a Wang, **aoyan Wang, Yongjuan Chen, Tingting Li & Shuang Song

  2. College of Life Sciences, Capital Normal University, Bei**g, 100048, China

    Zhiwei Yang

  3. College of Environmental Science and Engineering, Peking University, Bei**g, 100871, China

    Shuguang **e

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  1. **gyu Huang
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  2. **a Wang
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  3. **aoyan Wang
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Correspondence to **aoyan Wang.

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All sequences reported in this study are deposited in the NCBI Sequences Read Archive (SRA) under the Biological Project Accession number PRJNA741463.

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Huang, J., Wang, X., Wang, X. et al. Distribution characteristics of ammonia-oxidizing microorganisms and their responses to external nitrogen and carbon in sediments of a freshwater reservoir, China. Aquat Ecol 56, 841–857 (2022). https://doi.org/10.1007/s10452-022-09943-z

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