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LC–MS Method for Determination of Amphotericin B in Rabbit Tears and Its Application to Ocular Pharmacokinetic Study

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An Erratum to this article was published on 24 February 2011

Abstract

A rapid and sensitive LC–MS–MS method was developed for the quantification of amphotericin B in rabbit tears using natamycin as internal standard. The analyte and internal standard were extracted from the tear sample using a solid phase extraction method. Chromatographic separation was achieved on a Phenomenex Luna 3 μm CN column (100 × 2 mm, 3 μm) using 3.5 mM ammonium acetate (pH 4):methanol (10:90) as mobile phase. The assay was validated with a linear range of 0.1–3.2 μg mL−1 for amphotericin B using 10 μL of tear sample. The intra- and inter-day assay precision ranged from 2.49 to 4.37 and 2.17 to 5.59%, respectively, and intra- and inter-day assay accuracy was between from 0.27 to 3.32 and −0.51 to 3.72%, respectively. The method was successfully applied to the pharmacokinetic studies of amphotericin B eye drops in rabbit tears.

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Acknowledgments

Chetan Rathi and Hardik Chandasana are thankful to NIPER for the MS Fellowships during 2008–2010 and Ms. Rashmi Jaiswal for her help in carrying out the work.

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Authors and Affiliations

  1. Pharmacokinetics and Metabolism Division, Central Drug Research Institute, CSIR, Post box 173, Lucknow, 226001, India

    Rabi Sankar Bhatta, Devendra Kumar, Yashpal Singh Chhonker & Girish Kumar Jain

  2. Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research (NIPER), Raebareli, 229010, India

    Chetan Rathi & Hardik Chandasana

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  1. Rabi Sankar Bhatta
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  2. Chetan Rathi
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  3. Hardik Chandasana
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  4. Devendra Kumar
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  5. Yashpal Singh Chhonker
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  6. Girish Kumar Jain
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Correspondence to Rabi Sankar Bhatta.

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R. S. Bhatta, C. Rathi, H. Chandasana and D. Kumar contributed equally to this work.

An erratum to this article can be found at http://dx.doi.org/10.1007/s10337-011-1953-2

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Bhatta, R.S., Rathi, C., Chandasana, H. et al. LC–MS Method for Determination of Amphotericin B in Rabbit Tears and Its Application to Ocular Pharmacokinetic Study. Chromatographia 73, 487–493 (2011). https://doi.org/10.1007/s10337-010-1902-5

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  • DOI: https://doi.org/10.1007/s10337-010-1902-5

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