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Pin1 expression affects cell proliferation and apoptosis of SW620 cells in colorectal carcinoma

  • Published:
The Chinese-German Journal of Clinical Oncology

Abstract

Objective

The aim of our study was to investigate the effects of Pin1 reduction on SW620 cell proliferation and apoptosis in human colorectal carcinoma.

Methods

We constructed a plasmid of RNA interfering (shRNA) for Pin1 gene (pGenesl-1-Pin1), then the plasmid was transfected into colorectal carcinoma SW620 cells line by liposome mediation. The protein expression of Pin1 was tested by Western blotting. The proliferation rate was analyzed by MTT and the apoptotic rate of cells was tested by flow cytometry. In order to explain further the effect of Pin1 in SW620 cells, the protein level of Bcl-2 was analyzed by Western blotting.

Results

pGenesil-1-Pin1 plasmid was successfully constructed and confirmed by sequencing. The protein relative levels of Pin1 were 0.06 ± 0.04 for the P-shRNA/SW620 cells, and 0.32 ± 0.09 for the P-Con/SW620 cells. The cell growth rate of SW620 cells was slower while the apoptotic rate was increased after transfection with pGenesil-Pin1 plasmid, and the apoptotic rate was 12.38% ± 1.55% for the P-shRNA/SW620 group. At the same time, we found that the protein expression of Bcl-2 was also reduced. The results were 0.13 ± 0.04 for the P-shRNA/SW620 cells, and 0.36 ± 0.08 for the P-Con/SW620 cells.

Conclusion

Inhibited Pin1 expression may suppress the cell proliferation and promote apoptosis of colorectal carcinoma cells in vitro.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, 030001, China

    Yan Zhu, Liyuan Qin, Meining Li, Dong Zhang, Yuehong Zhang & Niuliang Cheng

Authors
  1. Yan Zhu
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  2. Liyuan Qin
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  3. Meining Li
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  4. Dong Zhang
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  5. Yuehong Zhang
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  6. Niuliang Cheng
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Corresponding author

Correspondence to Niuliang Cheng.

Additional information

Supported by grants from the National Natural Science Foundation (No. 81000948/H1606), Natural Science Foundation of Shanxi Province (No. 2010011047-1), and University Science Technology Development Project of Shanxi Province (No. 20091013).

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Zhu, Y., Qin, L., Li, M. et al. Pin1 expression affects cell proliferation and apoptosis of SW620 cells in colorectal carcinoma. Chin. -Ger. J. Clin. Oncol. 10, 100–104 (2011). https://doi.org/10.1007/s10330-011-0737-3

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  • DOI: https://doi.org/10.1007/s10330-011-0737-3

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