SpringerLink
Log in

Rapid enrichment of (homo)acetogenic consortia from animal feces using a high mass-transfer gas-lift reactor fed with syngas

  • Biotechnology Methods
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

A gas-lift reactor having a high mass transfer coefficient (k L a = 80.28 h−1) for a relatively insoluble gas (carbon monoxide; CO) was used to enrich (homo)acetogens from animal feces. Samples of fecal matter from cow, rabbit, chicken, and goat were used as sources of inoculum for the enrichment of CO and H2 utilizing microbial consortia. To confirm the successful enrichment, the Hungate roll tube technique was employed to count and then isolate putative CO utilizers. The results of this work showed that CO and H2 utilizing consortia were established for each inoculum source after 8 days. The number of colony-forming units in cow, rabbit, chicken, and goat fecal samples were 3.83 × 109, 1.03 × 109, 8.3 × 108, and 3.25 × 108 cells/ml, respectively. Forty-two colonies from the animal fecal samples were screened for the ability to utilize CO/H2. Ten of these 42 colonies were capable of utilizing CO/H2. Five isolates from cow feces (samples 5, 6, 8, 16, and 22) were highly similar to previously unknown (homo)acetogen, while cow-7 has shown 99 % similarity with Acetobacterium sp. as acetogens. On the other hand, four isolates from chicken feces (samples 3, 8, 10, and 11) have also shown high CO/H2 utilizing activity. Hence, it is expected that this research could be used as the basis for the rapid enrichment of (homo)acetogenic consortia from various environmental sources.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Abrini J, Naveau H, Nyns EJ (1994) Clostridium autoethanogenum sp. nov., an anaerobic bacterium that produces ethanol from carbon monoxide. Arch Microbiol 161(4):345–351

    Article  CAS  Google Scholar 

  2. Allen TD, Caldwell ME, Lawson PA, Huhnke RL, Tanner RS (2010) Alkalibaculum bacchi gen. nov., sp. nov., a novel CO oxidizing, ethanol producing acetogen isolated from livestock-impacted soil. Int J Syst Evol Microbiol 60(10):2483–2489

    Article  PubMed  CAS  Google Scholar 

  3. Belgiorno V, De Feo G, Della Rocca C, Napoli RMA (2001) Energy from gasification of solid wastes. Waste Manag 23(1):1–15

    Article  Google Scholar 

  4. Bharadwaj SS, Schmidt LD (1995) Catalytic partial oxidation of natural gas to syngas. Fuel Process Technol 42(2–3):109–127

    Article  CAS  Google Scholar 

  5. Bhatnagar L, Krzycki JA, Zeikus JG (1987) Analysis of hydrogen metabolism in Methanosarcina barkeri: regulation of hydrogenase and role of CO-dehydrogenase in H2 production. FEMS Microbiol Lett 41(3):337–343

    Article  CAS  Google Scholar 

  6. Chang IS, Kim DH, Kim BH, Shin PK, Sung HC, Lovitt RW (1998) CO fermentation of Eubacterium limosum KIST612. J Microbiol Biotechnol 8(2):134–140

    CAS  Google Scholar 

  7. Chang IS, Kim BH, Kim DH, Lovitt RW, Sung HC (1999) Formulation of defined media for carbon monoxide fermentation by Eubacterium limosum KIST612 and the growth characteristics of the bacterium. J Biosci Bioeng 88(6):682–685

    Article  PubMed  CAS  Google Scholar 

  8. Chang IS (2000) Carbon monoxide fermentation by Eubacterium limosum KIST612. Ph.D. thesis, University of Wales Swansea, Swansea, UK

  9. Chang IS, Kim BH, Lovitt RW, Bang JS (2001) Effect of partial pressure on cell-recycled continuous co fermentation by Eubacterium limosum KIST612. Process Biochem 37(4):411–421

    Article  CAS  Google Scholar 

  10. Coulson JM, Richardson JF, Backhurst JR, Harker JH (1999) Chemical engineering. Pergamon Press, Oxford

    Google Scholar 

  11. Demirbas A (2007) Progress and recent trends in biofuels. Energy Combust Sci 33(1):1–18

    Article  CAS  Google Scholar 

  12. Dubois JL (2011) Requirements for the development of a bioeconomy for chemicals. Curr Opin Environ Sustain 3(1–2):11–14

    Article  Google Scholar 

  13. Drake HL, Gößner AS, Daniel SL (2008) Old acetogens, new light. Ann NY Acad Sci 1125:100–128

    Article  PubMed  CAS  Google Scholar 

  14. Henstra AM, Sipma J, Rinzema A, Stams AJM (2007) Microbiology of synthesis gas fermentation for biofuel production. Curr Opin Biotechnol 18(3):200–206

    Article  PubMed  CAS  Google Scholar 

  15. Iino T, Mori K, Tanaka K, Suzuki K, Harayama S (2007) Oscillibacter valericigenes gen. nov., sp. nov., a valerate-producing anaerobic bacterium isolated from the alimentary canal of a Japanese corbicula clam. Int J Syst Evol Microbiol 57(8):1840–1845

    Article  PubMed  Google Scholar 

  16. Keim W (1986) C1 chemistry: potential and developments. Pure Appl Chem 58(6):825–832

    Article  CAS  Google Scholar 

  17. Kim BH, Bellows P, Datta R, Zeikus JG (1984) Control of carbon and electron Flow in Clostridium acetobutylicum fermentations: utilization of carbon monoxide to inhibit hydrogen production and to enhance butanol yields. Appl Environ Microbiol 48(4):764–770

    PubMed  CAS  Google Scholar 

  18. Klasson KT, Ackerson MD, Clausen EC, Gaddy JL (1993) Biological conversion of coal and coal-derived synthesis gas. Fuel 72(12):1673–1678

    Article  CAS  Google Scholar 

  19. Levy PF, Barnard GW, Garcia-Martinez DV, Sanderson JE, Wise DL (1981) Organic acid production from CO2/H2 and CO/H2 by mixed-culture anaerobes. Biotechnol Bioeng 23:2293–2306

    Article  CAS  Google Scholar 

  20. Liou JS-C, Balkwill DL, Drake GR, Tanner RS (2005) Clostridium carboxidivorans sp. nov., a solvent-producing clostridium isolated from an agricultural settling lagoon, and reclassification of the acetogen Clostridium scatologenes strain SL1 as Clostridium drakei sp. nov. Int J Syst Evol Microbiol 55(5):2085–2091

    Article  PubMed  CAS  Google Scholar 

  21. Miller TL, Wolin MJ (1974) A serum bottle modification of the Hungate technique for cultivating obligate anaerobes. Appl Microbiol 27(5):985–987

    PubMed  CAS  Google Scholar 

  22. Moench TT, Zeikus JG (1983) An improved preparation method for a titanium (III) media reductant. J Microbiol Methods 1(4):199–202

    Article  CAS  Google Scholar 

  23. Naik SN, Goud VV, Rout PK, Dalai AK (2010) Production of first and second generation biofuels: a comprehensive review. Renew Sustain Energy Rev 14(2):578–597

    Article  CAS  Google Scholar 

  24. Novaes E, Kirst M, Chiang V, Winter-Sederoff H, Sederoff R (2010) Lignin and biomass: a negative correlation for wood formation and lignin content in trees. Plant Physiol 154(2):555–561

    Article  PubMed  CAS  Google Scholar 

  25. Riggs SS, Heindel TJ (2006) Measuring carbon monoxide gas-liquid mass transfer in a stirred tank reactor for syngas fermentation. Biotechnol Prog 22(3):903–906

    Article  PubMed  CAS  Google Scholar 

  26. Shen GJ, Shieh JS, Grethlein AJ, Jain MK, Zeikus JG (1999) Biochemical basis for carbon monoxide tolerance and butanol production by Butyribacterium methylotrophicum. Appl Microbiol Biotechnol 51(6):827–832

    Article  CAS  Google Scholar 

  27. Tanner RS, Miller LM, Yang D (1993) Clostridium ljungdahlii sp. nov., an acetogenic species in clostridial ribosomal-RNA homology group-I. Int J Syst Bacteriol 43(2):232–236

    Article  PubMed  CAS  Google Scholar 

  28. Van Rossum G, Kersten SRA, Van Swaaij WPM (2007) Catalytic and noncatalytic gasification of pyrolysis oil. Ind Eng Chem Res 46(12):3959–3967

    Article  Google Scholar 

  29. Vega JL, Klasson KT, Kimmel DE, Clause EC, Gaddy JL (1991) Sulfur gas tolerance and toxicity of CO-utilizing and methanogenic bacteria. Appl Biochem Biotechnol 24–25:329–340

    Google Scholar 

  30. Zeikus JG, Lynd LH, Thompson TE, Krzycki JA, Weimer PJ, Hegge PW (1980) Isolation and characterization of a new, methylotrophic, acidogenic anaerobe, the Marburg strain. Curr Microbiol 3(6):381–386

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the Development of Biohydrogen Production Technology using the Hyperthermophilic Archaea program of the Ministry of Land, Transport, and Maritime Affairs, Republic of Korea. This work was also supported by the Research Program for Agricultural Science and Technology Development (project no. PJ008517032012) and by the Advanced Biomass R&D Center (ABC-2011-0031355) funded by the Ministry of Education, Science and Technology, and the Korea Environment Corporation.

Author information

Authors and Affiliations

  1. School of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST), 261 Cheomdan-gwagiro, Buk-gu, Gwangju, 500-712, Republic of Korea

    Shinyoung Park, Muhammad Yasin & In Seop Chang

  2. Jeju Global Research Center, Korea Institute of Energy Research, 200 Haemajihaean-ro, Gujwa-eub, Jeju, Jeju Special Self-Governing Province, 695-971, Republic of Korea

    Daehee Kim

  3. School of Civil, Environmental and Architectural Engineering, Korea University, Anam-dong, Sungbuk-ku, Seoul, 136-701, Republic of Korea

    Hee-Deung Park

  4. Gyeongnam Department of Environmental Toxicology & Chemistry, Korea Institute of Toxicology, 17 Jegok-Gil, Munsan-Eup, **ju, Gyeongsangnamdo, 660-844, Republic of Korea

    Chang Min Kang

  5. Department of Water and Sewage Support, Korea Environment Corporation, Gyeongseo-dong, Seo-gu, Incheon, 404-708, Republic of Korea

    Duk ** Kim

Authors
  1. Shinyoung Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Muhammad Yasin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Daehee Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hee-Deung Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Chang Min Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Duk ** Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. In Seop Chang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to In Seop Chang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Park, S., Yasin, M., Kim, D. et al. Rapid enrichment of (homo)acetogenic consortia from animal feces using a high mass-transfer gas-lift reactor fed with syngas. J Ind Microbiol Biotechnol 40, 995–1003 (2013). https://doi.org/10.1007/s10295-013-1292-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10295-013-1292-4

Keywords

Search

Navigation