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Low genetic diversity and significant population structuring in the relict Amentotaxus argotaenia complex (Taxaceae) based on ISSR fingerprinting

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Abstract

Amentotaxus, a genus of the Taxaceae, represents an ancient lineage that has long existed in Eurasia. All Amentotaxus species experienced frequent population expansion and contraction over periodical glaciations in Tertiary and Quaternary. Among them, Amentotaxus argotaenia complex consists of three morphologically alike species, A. argotaenia, Amentotaxus yunnanensis, and Amentotaxus formosana. This complex is distributed in the subtropical region of mainland China and Taiwan where many Pleistocene refugia have been documented. In this study, genetic diversity and population structuring within and between species were investigated based on the inter-simple sequence repeats (ISSR) fingerprinting. Mean genetic diversity within populations was estimated in three ways: (1) the percentage of polymorphic loci out of all loci (P) (2) Nei’s unbiased expected heterozygosity (He), and (3) Shannon’s index of phenotypic diversity. For a total of 310 individuals of 15 populations sampled from the three species, low levels of ISSR genetic variation within populations were detected, with P=4.66–16.58%, He=0.0176–0.0645 and Hpop=0.0263–0.0939, agreeing with their seriously threatened status. AMOVA analyses revealed that the differences between species only accounted for 27.38% of the total variation, whereas differences among populations and within populations were 57.70 and 14.92%, respectively, indicating substantial isolation between the patch-like populations. A neighbor-joining tree identified a close affinity between A. yunnanensis and A. formosana. Genetic drift due to small population size, plus limited current gene flow, resulted in significant genetic structuring. Low levels of intrapopulational genetic variation and considerable interpopulational divergence were also attributable to demographic bottlenecks during and/or after the Pleistocene glaciations.

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Acknowledgments

We thank Chao-Yi Deng, Guo-Sheng He, Yun-Fei Deng, Hua-Gu Ye, and Qing-Zhou Zheng for their help with material sampling. This work was supported by the National Natural Science Foundation of China (No. 30170070).

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Authors and Affiliations

  1. State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China

    Xue-Jun Ge

  2. South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China

    **an-Long Zhou & Zhong-Chao Li

  3. Division of Botany, Taiwan Endemic Species Research Institute, Chi-Chi , 551, Taiwan

    Tsai-Wen Hsu

  4. Department of Biology, Washington University, St. Louis, MO, 63130, USA

    Barbara A. Schaal

  5. Department of Life Sciences, Cheng Kung University, 1, University Road, Tainan, 701, Taiwan

    Tzen-Yuh Chiang

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  1. Xue-Jun Ge
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Correspondence to Tzen-Yuh Chiang.

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Ge, XJ., Zhou, XL., Li, ZC. et al. Low genetic diversity and significant population structuring in the relict Amentotaxus argotaenia complex (Taxaceae) based on ISSR fingerprinting. J Plant Res 118, 415–422 (2005). https://doi.org/10.1007/s10265-005-0235-1

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