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Oral bacterial genic expression detection in aneurysm wall of a French population sample – preliminary monocentric study

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Abstract

Introduction

The etiology of brain aneurysms remains poorly understood. Finnish research suggests that oral bacteria might contribute to the development and rupture of brain aneurysms. Previous studies by our team have not confirmed these findings, likely due to methodological differences. We aimed to replicate the Finnish study with a French population, using the same primers and comparing the results to internal controls.

Methods

We used RT-qPCR to retrospectively analyze the expression of oral bacterial genes in eight patients. During surgical procedures, four tissue types were consistently sampled from each patient: the aneurysmal wall, the superficial temporal artery (STA), the middle meningeal artery (MMA), and the dura mater (DM). Results were expressed as fold differences employing the 2−∆∆Ct method, and statistical analyses were performed accordingly.

Results

Our cohort included eight patients, evenly split into ruptured and unruptured groups. The sex distribution was balanced (4 females, 4 males). We observed DNA expression from oral bacteria in all sampled tissues; however, there were no significant differences between the ruptured and unruptured groups.

Conclusion

We detected oral bacterial gene expression in the aneurysmal wall, STA, MMA, and DM in a sample of French patients. Although limited by the small sample size, our results suggest a potential role for bacterial involvement in vascular invasiveness related to bacteremia. These findings do not definitively link oral bacteria to the pathogenesis of aneurysm development and rupture.

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Fig. 1
Fig. 2

Data availability

No datasets were generated or analysed during the current study.

Abbreviations

ACoA:

Anterior Communicating Artery

aSAH:

Acute SubArachnoid Hemorrhage

DM:

Dura Mater

DNA:

Deoxyribonucleic acid

DSA:

Digital Substraction Angiography

GCS:

Glascow Coma Scale

IA:

Intracranial Aneurysm

ICH:

Intracerebral Hemorrage

MCA:

Middle Cerebral Artery

MMA:

Middle Meningeal Artery

mRS:

Modified Rankin Score

PCoA:

Posterior Communication Artery

RNA:

Ribonucleic acid

RT-qPCR:

Real Time – quantitative Polymerization Chain Reaction

STA:

Superficial Temporal Artery

TLR:

Toll-like receptors

WFNS:

World Federation Neurosurgery Score

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Acknowledgements

We thank the OncoLille Institute. This work is supported by the INSERM Unit U1189-ONCO-THAI.

Funding

No funds, grants, or other support was received.

Author information

Authors and Affiliations

  1. Department of Neurosurgery, Lille University Hospital, Rue E. Laine, Hôpital Nord, Lille cedex, 59037, France

    Antoine Devalckeneer, Tuong Lu, Philippe Bourgeois & Rabih Aboukaïs

  2. Lille University, INSERM, CHU Lille, U1189-ONCO-THAI-Image Assisted Laser Therapy for Oncology, Lille, F-59000, France

    Antoine Devalckeneer, Guillaume Paul Grolez, Olivier Morales, Rabih Aboukaïs & Nadira Delhem

  3. Department of Pathology, Lille University Hospital, Hôpital Nord, France

    Claude-Alain Maurage

  4. Department of Microbiology, Lille University Hospital, Hôpital Nord, France

    Caroline Loiez & Thomas Menovsky

  5. University Lille, CNRS, Inserm, CHU Lille, UMR9020-U1277 - CANTHER - Cancer Heterogeneity Plasticity and Resistance to Therapies, Lille, F-59000, France

    Olivier Morales

  6. Department of Neurosurgery, Antwerpen University Hospital, Antwerp, Belgium

    Thomas Menovsky

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  1. Antoine Devalckeneer
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by A.D., T.L., N.D. and R.A. The first draft of the manuscript was written by A.D. and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Antoine Devalckeneer.

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Ethical approval

was waived by the local Ethics Committee of Lille University in view of the retrospective nature of the study and all the procedures being performed were part of the routine care. An IRB number was allocated by the Collège de Neurochirurgie whom approved the study (IRB00011687 College de neurochirurgie IRB #1 : 2023/34).

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Informed consent was obtained from all individual participants included in the study.

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Devalckeneer, A., Lu, T., Bourgeois, P. et al. Oral bacterial genic expression detection in aneurysm wall of a French population sample – preliminary monocentric study. Neurosurg Rev 47, 325 (2024). https://doi.org/10.1007/s10143-024-02548-0

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