Abstract
Management and improving saline-alkali land is necessary for sustainable agricultural development. We conducted a field experiment to investigate the effects of spraying lactic acid bacteria (LAB) on the cucumber and tomato plant soils. Three treatments were designed, including spraying of water, viable or sterilized LAB preparations to the soils of cucumber and tomato plants every 20 days. Spraying sterilized or viable LAB could reduce the soil pH, with a more obvious effect by using viable LAB, particularly after multiple applications. Metagenomic sequencing revealed that the soil microbiota in LAB-treated groups had higher alpha-diversity and more nitrogen-fixing bacteria compared with the water-treated groups. Both viable and sterilized LAB, but not water application, increased the complexity of the soil microbiota interactive network. The LAB-treated subgroups were enriched in some KEGG pathways compared with water or sterilized LAB subgroups, such as environmental information processing–related pathways in cucumber plant; and metabolism-related pathways in tomato plant, respectively. Redundancy analysis revealed association between some soil physico-chemical parameters (namely soil pH and total nitrogen) and bacterial biomarkers (namely Rhodocyclaceae, Pseudomonadaceae, Gemmatimonadaceae, and Nitrosomonadales). Our study demonstrated that LAB is a suitable strategy for decreasing soil pH and improving the microbial communities in saline-alkali land.
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Data availability
The entire sequence data set has been deposited in the National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA) database (Accession No: SRR16229770-SRR16229787). All data analyzed during this study are included in the additional files of this published article.
Abbreviations
- LAB :
-
Lactic acid bacteria
- PCoA :
-
Principal coordinates analysis
- NMDS :
-
Nonmetric multidimensional scales ordination
- RDA :
-
Redundancy analysis
- PICRUSt :
-
Phylogenetic investigation of communities by reconstruction of unobserved states
- PPM :
-
Plant probiotic microorganisms
- OTUs :
-
Operational taxonomic units
- LEfSe :
-
Linear discriminant analysis (LDA) effect size
- KEGG :
-
Kyoto Encyclopedia of Genes and Genomes
- KO :
-
KEGG Ontology
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This study was supported by the Science and Technology Project of College of Food Science and Engineering, Inner Mongolia Agricultural University (SPKJ201905).
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Conceived and designed the experiments: ZZ; MB.
Performed field experiments and managed the project: JQH; MB.
Analyzed data: HT, LJY, ZXZ.
Wrote the manuscript: HT.
Critical evaluation and extensive revision of the manuscript: LYK.
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Tang, H., Zhong, Z., Hou, J. et al. Metagenomic analysis revealed the potential of lactic acid bacteria in improving natural saline-alkali land. Int Microbiol 27, 311–324 (2024). https://doi.org/10.1007/s10123-023-00388-4
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DOI: https://doi.org/10.1007/s10123-023-00388-4