Abstract
Sepsis causes high mortality in intensive care units. Although there have been many studies on the gut microbiota in patients with sepsis, the impact of sepsis on the gut microbiota has not been directly determined because the treatment of sepsis also affects the gut microbiota. Therefore, we designed this animal experiment to explore gut microbiota alterations during sepsis. Mice were divided into two groups, mice that survived less than 3 days and mice that survived more than 3 days. Fecal samples collected on the day of cecal ligation and puncture (CLP), as well as on the 3rd and 7th days after CLP, were subjected to microbial community analysis and nontargeted metabolomics analysis. The results showed significantly lower bacterial diversity in fecal samples after CLP. At the genus level, the fecal samples obtained on the 3rd and 7th days after CLP exhibited significantly increased relative abundances of Bacteroides, Helicobacter, etc., and significantly decreased relative abundances of Alloprevotella, Prevotella, etc. Innate metabolite levels were significantly different in mice that survived less than 3 days and mice that survived more than 3 days. In conclusion, CLP-induced sepsis in mice changes the structure of the gut microbiome, and innate metabolites affect the prognosis of septic mice.
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Data availability
The datasets generated during the current study are available in the NCBI repository, https://www.ncbi.nlm.nih.gov/bioproject/PRJNA758244.
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This work was supported by the National Natural Science Foundation of China (No. 81770276, U20A20366).
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All authors participated in the design and interpretation of the studies, the analysis of the data and the review of the manuscript. Kaijiang Yu, Changsong Wang and **aohui Ma designed the experiments. **aohui Ma, **aonan Jia, Yahui Peng and Xueting Li conducted the experiments. **aohui Ma analyzed the data and wrote the manuscript.
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Experiments were performed under a project license (NO. KY2018-02) granted by regional ethics board of Harbin Medical University Cancer Hospital on December 2018, in compliance with chinese guidelines for the care and use of animals. The chairperson of the ethics committee is Changhong Zhao.
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Ma, X., Jia, X., Peng, Y. et al. Gut microbiota disruption during sepsis and the influence of innate metabolites on sepsis prognosis. Int Microbiol 26, 929–938 (2023). https://doi.org/10.1007/s10123-023-00349-x
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DOI: https://doi.org/10.1007/s10123-023-00349-x