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Metabolic changes of Issatchenkia orientalis under acetic acid stress by transcriptome profile using RNA-sequencing

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Abstract

Issatchenkia orientalis (I. orientalis) is tolerant to various environmental stresses especially acetic acid stress in wine making. However, limited literature is available on the transcriptome profile of I. orientalis under acetic acid stress. RNA-sequence was used to investigate the metabolic changes due to underlying I. orientalis 166 (Io 166) tolerant to acetic acid. Transcriptomic analyses showed that genes involved in ergosterol biosynthesis are differentially expressed under acetic acid stress. Genes associated with ribosome function were downregulated, while energy metabolism-related genes were upregulated. Moreover, Hsp70/Hsp90 and related molecular chaperones were upregulated to recognize and degrade misfolded proteins. Compared to Saccharomyces cerevisiae, transcriptomic changes of Io 166 showed many similarities under acetic acid stress. There were significant upregulation of genes in ergosterol biosynthesis and for the application of wine production.

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Funding

Thanks to the financial support of the National Natural Science Foundation, China (NNSF No. 31471709), Public Welfare Project of Zhejiang (LGN18C200018), and K.C. Wong Magna Fund in Ningbo University.

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Author notes

  1. Yueqin Li and Yingdi Li contributed equally to the study in the name of first author.

Authors and Affiliations

  1. Department of Food Science and Engineering, School of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, 315832, People’s Republic of China

    Yueqin Li, Yingdi Li, Ruoyun Li, Lianliang Liu, Yingjie Miao, Peifang Weng & Zufang Wu

  2. Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Ningbo University, Ningbo, 315832, People’s Republic of China

    Lianliang Liu & Zufang Wu

  3. School of Life Science, Taizhou University, Taizhou, 318000, People’s Republic of China

    Yingjie Miao

Authors
  1. Yueqin Li
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  2. Yingdi Li
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  3. Ruoyun Li
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  4. Lianliang Liu
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Contributions

All authors contributed to the study’s conception and design. Yueqin Li and Yingdi Li contributed equally to the study in the name of first author. Early experiments (including material preparation) and testing were mainly in charge of Yingdi Li. Yueqin Li analyzes the protein-protein interaction network and the effect of heat shock proteins and protein folding on yeast cells. Moreover, she is responsible for writing and revising the manuscript in the whole process. Ruoyun Li provided data analysis support. Lianliang Liu and Zufang Wu designed and supervised the manuscript. Yingjie Miao, Peifang Weng, and all authors commented on previous versions of the document. All authors read and approved the final manuscript.

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Correspondence to Lianliang Liu or Zufang Wu.

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The original online version of this article was revised: The authors regret that there is a mistake in the first author’s name in the original article. The correct first author’s name is incorporated in this erratum article.

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Li, Y., Li, Y., Li, R. et al. Metabolic changes of Issatchenkia orientalis under acetic acid stress by transcriptome profile using RNA-sequencing. Int Microbiol 25, 417–426 (2022). https://doi.org/10.1007/s10123-021-00217-6

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