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Fractionated low-level laser irradiation on breast cancer (MCF 7 cells) treatment

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Abstract

Breast cancer is responsible for one of the top leading causes of cancer deaths among women. Radiotherapy (RT) uses high energy radiation to kill cancer cells, but this method has been reportedly linked to risks of toxicity. Post-therapeutic relapse from RT believed to be caused by its toxicity is one of the challenges encountered during tumour therapy. Therefore, further attention should be devoted to develo** novel anti-tumour therapeutic approaches. The role of low-level laser therapy (LLLT) in breast cancer management is to alleviate the side effects arising from RT, instead of acting against the tumour cells directly. This study investigated the effects of low-level laser (532 nm), as well as single and fractionated irradiation, on breast cancer MCF 7 cell line. Additionally, this study assessed the most effective laser parameter for fractionated irradiation. The MCF 7 cells were irradiated with green laser power at 1.5, 45.0, and 100.0 mW with a spot size diameter of 0.7 mm for 1, 5, 10, and 15 min. The irradiation was carried out in single, double, and triple fractionation separated by 5- and 10-min intervals in between the fractional regimes. The laser output of 100 mW showed a promising potential in killing cells with single fractionation. However, as the irradiation was fractionated into two, power of 1.5 mW appeared to be more effective in cell death, which contributed to the lowest percentage cells viable of 31.4% recorded in the study. It was proven that fractionated regime was more successful in tumour cell death.

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Acknowledgements

The authors wish to thank staff of Institute for Research in Molecular Medicine (INFORMM) for their assistance and support for this research work.

Funding

This research was supported by a research grant (RUI 1001/PFIZIK/8011111) from Universiti Sains Malaysia (USM).

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Authors and Affiliations

  1. School of Physics, Universiti Sains Malaysia 11800, Penang, Malaysia

    Nursakinah Suardi, Pegah Moradi Khaniabadi, Aijesta Taggo, Siti Farrah Mursyida Zulbaharin & Sylvester Jande Gemanam

  2. Department of Radiology and Molecular Imaging, College of Medicine and Health Science, Sultan Qaboos University, 123, Al Khod, PO. Box: 35, Muscat, Oman

    Pegah Moradi Khaniabadi

  3. Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800, Penang, Malaysia

    Daruliza Kernain Mohd Azman

  4. Department of Physics, Benue State University, PMB 102119, Makurdi, Nigeria

    Sylvester Jande Gemanam

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  1. Nursakinah Suardi
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Correspondence to Nursakinah Suardi or Pegah Moradi Khaniabadi.

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Suardi, N., Khaniabadi, P.M., Taggo, A. et al. Fractionated low-level laser irradiation on breast cancer (MCF 7 cells) treatment. Lasers Med Sci 37, 1265–1271 (2022). https://doi.org/10.1007/s10103-021-03384-0

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