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Evaluating Fourier-transform infrared spectroscopy with IR Biotyper as a faster and simpler method for investigating the sources of an outbreak of legionellosis

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Abstract

Fourier-transform infrared (FTIR) spectroscopy using the IR Biotyper and core genome single nucleotide polymorphism (cgSNP) analysis were performed on 12 Legionella isolates associated with an outbreak at a spa house in Kanagawa Prefecture, Japan, and 3 non-outbreak isolates. The discriminative power of FTIR spectroscopy for 48-h incubation conditions of L. pneumophila in this outbreak was lower than cgSNP-based ty** but higher than serogroup ty**. FTIR spectroscopy could screen outbreak isolates from a group of genetically related isolates and may be useful as an initial ty** method in Legionella outbreak investigations.

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Data availability

The whole genome sequencing data generated in this study can be found in the BioProject PRJDB16933, and BioSample SAMD00656567-SAMD00656578 and SAMD00738127-SAMD00738129.

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Funding

This study was supported (partially) by a Health and Labor Sciences Research Grant (JPMH22LA1008) from the Ministry of Health, Labor and Welfare, Japan.

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Authors and Affiliations

  1. Department of Microbiology, Kanagawa Prefectural Institute of Public Health, 1-3-1 Shimomachiya, Chigasaki, Kanagawa, 253-0087, Japan

    Naoki Nakajima & Michio **nai

  2. Department of Parasitology, National Institute of Infectious Diseases, Tokyo, Japan

    Shinji Izumiyama

  3. Faculty of Veterinary Medicine, Okayama University of Science, Okayama, Japan

    Toshiro Kuroki

Authors
  1. Naoki Nakajima
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  2. Michio **nai
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  3. Shinji Izumiyama
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  4. Toshiro Kuroki
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Contributions

NN and MJ contributed to the conception of the study. NN performed the experiments and data analysis. NN wrote the first draft of the manuscript. NN, MJ, SI, and TK revised the manuscript and approved the final draft.

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Correspondence to Naoki Nakajima.

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Nakajima, N., **nai, M., Izumiyama, S. et al. Evaluating Fourier-transform infrared spectroscopy with IR Biotyper as a faster and simpler method for investigating the sources of an outbreak of legionellosis. Eur J Clin Microbiol Infect Dis 43, 991–997 (2024). https://doi.org/10.1007/s10096-024-04781-3

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  • DOI: https://doi.org/10.1007/s10096-024-04781-3

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