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Inulinase from Rhodotorula mucilaginosa: immobilization and application in the production of fructooligosaccharides

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Abstract

The crude extract containing inulinase from Rhodotorula mucilaginosa was obtained by submerged fermentation. Inulinase was immobilized on chicken eggshell by physical adsorption and covalent crosslinking, using glutaraldehyde as a crosslinking reagent, and Celite by adsorption. Fructooligosaccharides production was performed using immobilized inulinase (5%, w/v) and inulin substrate solution under experimental conditions evaluated through Doehlert experimental design. The production of inulinase was optimized for concentrations of D-glucose and yeast extract at 12.5 and 0.5 g/L, respectively, resulting in an optimal activity of 0.62 U. The optimal pH and temperature for enzyme activity were 8.0 and 75 °C, respectively, leading to an optimal activity of 3.54 U. The highest immobilization efficiency (46.27%) was obtained upon immobilization on Celite. Immobilization by adsorption to eggshell allowed for specific activity of 4.15 U/g, and adsorption to Celite resulted in specific activity of 3.70 U/g. The highest titer in fructooligosaccharides was obtained with an initial inulin concentration of 250 g/L (25%, w/v), and a reaction time of 16 h. Hence, immobilized inulinase proved to be a promising catalyst for fructooligosaccharides production since the formulation is performed through a simple, low-cost, and large-scale applicable methodology.

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Acknowledgements

This work was supported by FINEP (Financier of Studies and Projects), CAPES (Coordination of Improvement of Higher Level Personnel), CNPq (National Research Council), and FAPESB (Foundation for Research Support of the State of Bahia). We also thank the Biotechnology Graduate Program of the State University of Feira de Santana (UEFS/FIOCRUZ).

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Authors and Affiliations

  1. Laboratory of Enzymology and Fermentation Technology, Department of Health, State University of Feira de Santana, Av Transnordestina, Km 0, BR 116, Feira de Santana, Bahia, 44036-900, Brazil

    Geise Camila de Araujo Ribeiro & Sandra Aparecida de Assis

  2. DREAMS and Faculty of Engineering, Lusófona University, Lisboa, Portugal

    Pedro Fernandes

  3. Department of Bioengineering, IBB - Institute for Bioengineering and Biosciences, Instituto Superior Técnico, University of Lisbon, Lisboa, Portugal

    Pedro Fernandes

  4. Laboratory of Vegetal Bioprospection, Department of Health, State University of Feira de Santana, Av Transnordestina, Km 0, BR 116, Feira de Santana, Bahia, 44036-900, Brazil

    Dayse Alessandra Almeida Silva & Hugo Neves Brandão

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  1. Geise Camila de Araujo Ribeiro
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  2. Pedro Fernandes
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  3. Dayse Alessandra Almeida Silva
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  5. Sandra Aparecida de Assis
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Correspondence to Sandra Aparecida de Assis.

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de Araujo Ribeiro, G.C., Fernandes, P., Silva, D.A.A. et al. Inulinase from Rhodotorula mucilaginosa: immobilization and application in the production of fructooligosaccharides. Food Sci Biotechnol 30, 959–969 (2021). https://doi.org/10.1007/s10068-021-00931-x

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  • DOI: https://doi.org/10.1007/s10068-021-00931-x

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