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Anti-adipogenic effects in 3T3-L1 cells of acetone extracts and fractions from Styrax japonica fruit

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Abstract

Reduction in reactive oxygen species (ROS) production facilitated by Styrax japonica (SJ) extracts and fractions for inhibition of obesity development through inhibition of adipogenesis was investigated. Treatment of cells with SJ extracts and fractions did not result in changes in cell viability. SJ extracts and fractions inhibited lipid accumulation in a dose-dependent manner during adipogenesis. Expressions of PPARγ and C/EBPα, key adipogenic markers, were decreased in cells treated with SJ extracts and fractions. Inhibitory effects of SJ extracts and fractions were the most powerful in the early stages of differentiation (days 0-2). Intracellular ROS production was decreased in cells treated with SJ extracts and fractions. SJ extracts and fractions can inhibit adipogenesis via reduced ROS levels, which involves down-regulation of adipogenic transcription factors.

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Authors and Affiliations

  1. Gyeonggi Institute of Science and Technology Promotion, Suwon, Gyeonggi, 443-270, Korea

    ** Kyu Kim & Joa Sub Oh

  2. Institute of Natural Medicine, Hallym University, Chuncheon, Gangwon, 200-702, Korea

    Ha** Nam & Jun Gyo Suh

  3. Department of Medical Genetics, College of Medicine, Hallym University, Chuncheon, Gangwon, 200-702, Korea

    Yoo Yeon Kim, Harry Jung, Yoon Soo Choi & Jun Gyo Suh

  4. College of Pharmacy, Dankook University, Cheonan, Chungnam, 330-714, Korea

    Joa Sub Oh

  5. College of Forest & Environmental Sciences, Kangwon National University, Chuncheon, Gangwon, 200-701, Korea

    Su Kyoung Chun

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  1. ** Kyu Kim
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Correspondence to Jun Gyo Suh.

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Kim, J.K., Nam, H., Kim, Y.Y. et al. Anti-adipogenic effects in 3T3-L1 cells of acetone extracts and fractions from Styrax japonica fruit. Food Sci Biotechnol 24, 1513–1521 (2015). https://doi.org/10.1007/s10068-015-0195-8

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  • DOI: https://doi.org/10.1007/s10068-015-0195-8

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