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Advanced platelet rich fibrin demonstrates improved osteogenic induction potential in human periodontal ligament cells, growth factor production and mechanical properties as compared to leukocyte and platelet fibrin and injectable platelet rich fibrin

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Abstract

Objectives

This cross-sectional invitro research aimed to compare and contrast the macroscopic and microscopic, mechanical and biochemical features of leukocyte-rich platelet-rich fibrin, advanced platelet-rich fibrin, and injectable platelet-rich fibrin.

Materials and methods

In all, 150 samples were taken from males aged 18 to 25 with good systemic health (n = 50 each for i-PRF, A-PRF, and L-PRF). The samples were assessed for clot length, clot width, membrane length and width. Microscopic parameters assessed were the distribution of cells and fibrin structure. Mechanical tests were performed for tensile strength using a universal testing machine and growth factor analysis was performed for platelet derived growth factor (PDGF), vascular endothelial growth factor (VEGF), and transforming growth factor (TGF)- \(\beta\) on Days 1, 3 and 7 using commercially available ELISA kits. The osteogenic potential was analyzed in a culture of human periodontal ligament cells for 21 days using cell viability assay, alkaline phosphatase formation and alizarin red staining for mineralization.

Results

L-PRF demonstrates statistically superior clot length, width, weight, membrane length, width and weight in comparison to A-PRF (p < 0.05). L-PRF demonstrates a denser fibrin structure in comparison to A-PRF and i-PRF (p < 0.05). The cells in L-PRF are most commonly situated in the proximal of the clot where as they are distributed in the proximal and middle aspect for A-PRF(p < 0.05). A-PRF demonstrates the highest tensile strength followed by L-PRF (p < 0.05). When growth factor release was evaluated, A-PRF showed noticeably increased release of all growth factors, namely PDGF-BB, TGF-ß, and VEGF, in comparison to i-PRF and L-PRF (p < 0.05). On days 7 and 14, the cell viability of human periodontal ligament cells in co-culture with A-PRF was statistically substantially greater than that of L-PRF and i-PRF (p < 0.05). Alkaline phosphatase levels were statistically substantially higher in A-PRF, followed by i-PRF and L-PRF on days 14 and 21 (p < 0.05). After 21 days of culture, A-PRF treated cultures had much more Alizarin Red staining than L-PRF and i-PRF cultures did (p < 0.05).

Conclusion

It was determined that although L-PRF exhibits greater size and weight in comparison to A-PRF and i-PRF, A-PRF has superior mechanical properties, increased growth factor releases of TGF-b, PDGF-BB, and VEGF as well as superior cell viability, alkaline phosphatase production, and mineralization on human periodontal ligament cells.

Clinical relevance

Based on these findings, A-PRF can be recommended for improved delivery of growth factors and osteogenesis whereas L-PRF is better-suited for applications relying on the size of membrane.

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Data availability

Data supporting the findings of this study are available upon request from the corresponding author. The data are not publicly available due to privacy or ethical restrictions, but can be shared upon request. Requests for access to the data should be directed to srucha2k@gmail.com.

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Acknowledgements

Dr. K G Bhat, Maratha Mandal Dental College, Belgaum, Karnataka India for carrying out ELISA and Cell culture analysis.

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  1. Department of Periodontics, Bapuji Dental College & Hospital, MCC B Block, Davangere, Karnataka, India, 577004

    Rucha Shah, Triveni M. G., Raison Thomas & Tarun Kumar A. B.

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  1. Rucha Shah
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Contributions

Shah R: contributed to Conceptualization, Methodology,, Investigation, Resources, Data Collection, Statistics, Writing – Original Draft Preparation, Writing – Review & Editing, Visualization

MG Triveni: contributed to Conceptualization, Methodology, Data Collection, Writing – Review & Editing, Visualization, Approval of final draft

Thomas R: contributed to Conceptualization, Methodology, Data Collection, Statistics, Writing – Review & Editing, Visualization

AB Tarun Kumar: contributed to Conceptualization, Methodology, Data Collection, Writing – Review & Editing, Visualization

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Correspondence to Rucha Shah.

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Shah, R., M. G., T., Thomas, R. et al. Advanced platelet rich fibrin demonstrates improved osteogenic induction potential in human periodontal ligament cells, growth factor production and mechanical properties as compared to leukocyte and platelet fibrin and injectable platelet rich fibrin. Oral Maxillofac Surg 28, 413–424 (2024). https://doi.org/10.1007/s10006-023-01160-8

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