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Characterization and biological activities of a novel exopolysaccharide produced from Geobacillus thermodenitrificans HBB 111 strain

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Abstract

In this study, EPS production conditions of Geobacillus thermodenitrificans HBB 111, a thermophilic microorganism, were optimized and the amount of produced EPS (EPS 111) was found to be 44.0 mg/L. EPS 111 was purified using ion exchange chromatography and gel filtration chromatography, and a single type of exopolysaccharide was obtained. The structure of the purified EPS 111 was evaluated by TLC, FTIR, NMR, and GC–MS, and it was observed that it contained hexose (glucose, fructose, galactose and mannose) and pentose sugars. From the SEM photographs, it was understood that EPS 111 had an amorphous, rough, and layered structure. It was found that purified EPS 111 had low cytotoxicity (2.3%) and exhibited high antioxidant activity and remarkable antidiabetic, prebiotic and fibrinolytic activities. It is very valuable that the purified EPS 111 in this study offers multiple biological activities compared to the thermophilic EPSs reported in the literature and has a high potential for use in biotechnological and biomedical fields.

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The data of this study is available upon request.

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Acknowledgements

This work was supported by Adnan Menderes University (Turkey) Research Fund, by the project number FEF-20026. Mehmet Aytar thanks the Higher Education Council of Turkey (YOK) for 100/2000 PhD scholarship.

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Authors and Affiliations

  1. Department of Biology, Institute of Science, Aydın Adnan Menderes University, Aydın, Türkiye

    Mehmet Aytar

  2. Department of Biology, Faculty of Science, Aydın Adnan Menderes University, Aydın, Türkiye

    Gamze Başbülbül

  3. Department of Chemistry, Faculty of Science, Aydın Adnan Menderes University, Aydın, Türkiye

    Deniz Aktaş Uygun

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  1. Mehmet Aytar
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  2. Gamze Başbülbül
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Correspondence to Deniz Aktaş Uygun.

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Aytar, M., Başbülbül, G. & Uygun, D.A. Characterization and biological activities of a novel exopolysaccharide produced from Geobacillus thermodenitrificans HBB 111 strain. Extremophiles 28, 27 (2024). https://doi.org/10.1007/s00792-024-01344-4

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