Abstract
A novel alkaliphilic sulfate-reducing bacterium, strain M08butT, was isolated from a salsa lake of terrestrial mud volcano (Taman Peninsula, Russia). Cells were rod-shaped, motile and Gram-stain-negative. The temperature range for growth was 15–42 °C (optimum at 30 °C). The pH range for growth was 7.0–11.0, with an optimum at pH 8.5–9.0 Strain M08butT used sulfate, thiosulfate, sulfite, dimethyl sulfoxide and arsenate as electron acceptors. Acetate, formate, butyrate, fumarate, succinate, glycerol and pyruvate were utilized as electron donors with sulfate. Fermentative growth was observed with fumarate, pyruvate, crotonate. Strain M08butT grew chemolithoautotrophically with H2 and CO2. The G + C content of the genomic DNA was 60.1%. The fatty acid profile of strain M08butT was characterized by the presence of anteiso-C15:0 as the major component (68.8%). The closest phylogenetic relative of strain M08butT was Desulfatitalea tepidiphila (the order Desulfobacterales) with 96.3% 16S rRNA gene sequence similarity. Based on the phenotypic, genotypic and phylogenetic characteristics of the isolate, strain M08butT is considered to represent a novel species of the genus Desulfatitalea, with proposed name Desulfatitalea alkaliphila sp. nov. The type strain of Desulfatitalea alkaliphila is M08butT (= KCTC 25382T = VKM B-3560T = DSM 113909T = JCM 39202T = UQM 41473T).
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This work was funded by the Russian Science Foundation, grant number 22–14–00011 (MK, AM, AS, isolation and characterization) and by the Ministry of Science and Higher Education of the Russian Federation (genome sequencing and annotation).
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The GenBank accession number for the 16S rRNA gene sequence of strain M08butT is OP380633. This Whole Genome Shotgun project has been deposited at DDBJ/ENA/GenBank under the accession JALJRB000000000.
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Khomyakova, M.A., Merkel, A.Y., Segliuk, V.S. et al. Desulfatitalea alkaliphila sp. nov., an alkalipilic sulfate- and arsenate- reducing bacterium isolated from a terrestrial mud volcano. Extremophiles 27, 12 (2023). https://doi.org/10.1007/s00792-023-01297-0
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DOI: https://doi.org/10.1007/s00792-023-01297-0