Abstract
Objective
To identify the effect of two chitosan solutions on the release of root dentin matrix proteins and to describe the chemical changes observed following conditioning with chelating agents.
Materials and methods
The release of dentin sialoprotein (DSP), transforming growth factor-beta 1 (TGF-β1), vascular endothelial growth factor (VEGF), and platelet-derived growth factor-BB (PDGF-BB) with different chelating agents, including ethylenediaminetetraacetic acid (EDTA), chitosan solution (CS), and nanoparticulate chitosan (CSnp), was investigated. DSP was quantified using an enzyme-linked immunosorbent assay (ELISA). TGF-β1, VEGF, and PDGF-BB were quantified using a cytokine bead panel (CBA). Raman spectroscopy was performed to identify surface chemical changes. Statistical analysis was performed using Kruskal–Wallis test with Mann–Whitney–Wilcoxon rank-sum test (p < 0.05).
Results
TGF-β1, VEGF, and DSP solubilized in all irrigants tested. CSnp showed the highest concentration of DSP. PDGF-BB did not exceed the detection limits. Raman spectroscopy revealed a decrease in the phosphate and carbonate peaks, representing the chelating effect of EDTA, CS, and CSnp. Additionally, CSnp showed the greatest preservation of the amide I and III content.
Conclusion
Proteins can be released from dentin via EDTA, CS, and CSnp conditioning. Raman spectroscopic revealed changes in the inorganic content of the root dentin after chelation. Furthermore, use of CSnp facilitated a preservation of the organic content.
Clinical relevance
Chelation allows the release of proteins, justifying the use of chelating agents in regenerative endodontics. The chitosan–dentin matrix interaction also promotes the protection of the organic content as an additional benefit to its protein releasing effect.
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Funding
This research was funded by the Universidad Nacional de Colombia through the Convocatoria para el fortalecimiento de alianzas interdisciplinarias de investigación y creación artística sede Bogotá 2018, Project Code: 41999.
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Quijano-Guauque, S., Bernal-Cepeda, L.J., Delgado, F.G. et al. Effect of chitosan irrigant solutions on the release of bioactive proteins from root dentin. Clin Oral Invest 27, 691–703 (2023). https://doi.org/10.1007/s00784-022-04787-1
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DOI: https://doi.org/10.1007/s00784-022-04787-1