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Correlation between vitamin D metabolites and rheumatoid arthritis with osteoporosis by ultra-high-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS)

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Abstract

Introduction

Our aim is to study the correlation between vitamin D metabolites and osteoporosis in rheumatoid arthritis (RA) by ultra-high-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS). At the same time, other influencing factors and serum biomarkers of osteoporosis in patients with RA were studied.

Materials and methods

Patients with RA admitted from January 2020 to December 2020 were selected at our hospital. The subjects were divided into the normal bone mineral density (BMD), osteopenia, and osteoporosis groups. The differences of vitamin D (VD) metabolites among groups were compared. The Pearson correlation coefficient was used to analyze the relationship between BMD and various parameters. The relationship between BMD and influencing factors was studied by a multiple linear regression equation.

Results

A total of 287 patients with RA were included. RA patients with 25-hydroxy vitamin D [25(OH)D] deficiency accounted for 43.63% and 25(OH)D insufficient levels accounted for 31.37%. There were 31 cases (10.80%) in the normal BMD group, 161 cases (56.10%) in the osteopenia group, and 95 cases (33.10%) in the osteoporosis group. The BMD of L1-4 (T- score) was negatively correlated with age (P < 0.05), course of disease (P < 0.05), and erythrocyte sedimentation rate (ESR) (P < 0.05), and positively correlated with 25(OH)D3 (P < 0.05). The multiple linear regression model results showed that age and 25(OH)D3 were independent predictors of BMD; this explained 22.11% of the total variation.

Conclusions

VD deficiency and insufficient are common in RA patients. RA patients can be appropriately supplemented with VD. VD3 may be a better choice.

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Availability of data and materials

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

We would like to express our gratitude to all those who helped us during the writing of this manuscript.

Funding

Science and Technology Program of Sichuan Province (2017SZ0148).

Author information

Author notes

  1. **mei Zou and Lungang Zhu have contributed equally to this work.

Authors and Affiliations

  1. Department of Rheumatology and Immunology, Mianyang Central Hospital, No.12 Changjiaxiang, **gzhong Street, Fucheng District, Mianyang, 621000, China

    **mei Zou, **g Yang, Siyin Li, Jiaang Luo, Min Li, Yan Ren, Jianling Dong, Yu Zhang & Lan Tian

  2. Department of Emergency, Mianyang Central Hospital, Mianyang, China

    Lungang Zhu

  3. Department of Clinical Laboratory, Mianyang Central Hospital, Mianyang, China

    Jiafu Feng

Authors
  1. **mei Zou
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  2. Lungang Zhu
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  4. Jiafu Feng
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  5. Siyin Li
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  10. Yu Zhang
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  11. Lan Tian
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Contributions

Conception and design: JZ and LZ. Administrative support: JY, SL and YZ. Provision of study materials or patients: JZ, LZ. Collection and assembly of data: JL, ML and LT. Data analysis and interpretation: YR and JD. Manuscript writing: JZ and LZ. Manuscript revision: JF. Final approval of manuscript: all authors.

Corresponding author

Correspondence to **g Yang.

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Conflict of interest

All of the authors had no any personal, financial, commercial, or academic conflicts of interest separately.

Ethics approval

This study was conducted in accordance with the Declaration of Helsinki. This study was conducted with approval from the Ethics Committee of our Hospital No.S2016051.

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Written informed consent was obtained from all participants.

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Zou, J., Zhu, L., Yang, J. et al. Correlation between vitamin D metabolites and rheumatoid arthritis with osteoporosis by ultra-high-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS). J Bone Miner Metab 40, 696–703 (2022). https://doi.org/10.1007/s00774-022-01337-3

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  • DOI: https://doi.org/10.1007/s00774-022-01337-3

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