Abstract
Meyna spinosa Roxb. ex Link, locally known as “Haloo”, was sampled from Surgana, Nasik, and is reported to have many ethnobotanical uses. This study aims at analyzing and isolating the bioactive components responsible for the antimicrobial activity of the plant’s leaf methanolic extracts. Antimicrobial activity was studied using ditch-plate technique and bioautography. Ditch-plate method showed antimicrobial activity against Staphylococcus aureus. Bioautography in which thin-layer chromatography (TLC) is coupled to bioassay technique showed antimicrobial activity at the application spot against Pseudomonas aeruginosa. The compound/s were collected and concentrated by preparative TLC. The concentrated bioautography isolate (BI) and the leaf methanolic extract (ML) of M. spinosa Roxb. ex Link were subjected to liquid chromatography (LC) followed by electrospray ionization‒quadrupole-time-of-flight‒mass spectrometry (ESI‒Q-TOF‒MS), to compare the bioactive components. It was found that BI showed 10-deoxymethymycin, dihydrodeoxystreptomycin, 5E,8E,11E-hexadecatrienoic acid, deferoxime that have antibacterial properties. ML in addition shows quercetin, kaempferol, 3,4,5-trihydroxystilbene, swietenine, which have antioxidant and antimicrobial activities. We conclude that the antimicrobial activity exhibited by the ML was due to the synergistic action of all these compounds. The minimum inhibitory concentration (MIC) of ML by microdilution revealed to be 375 mg/mL for S. aureus and 125 mg/mL for P. aeruginosa.
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Acknowledgements
We would like to acknowledge the HRLC-MS Lab Staff at SAIF-IIT Bombay for providing LC‒ESI‒Q-TOF‒MS services for our work and TLC and preparative TLC service at Anchrom Enterprises Pvt. Ltd., Mulund, Mumbai.
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All authors contributed to the study conception and design. SK, SD and AP prepared the material, collected the data and its analysis. SK prepared the first draft of the manuscript and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Kadirvelu, S., Damle, S. & Pillai, A. Bioautography and liquid chromatography‒mass spectrometry studies of Meyna spinosa Roxb. ex Link leaf methanolic extracts. JPC-J Planar Chromat 34, 403–410 (2021). https://doi.org/10.1007/s00764-021-00134-4
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DOI: https://doi.org/10.1007/s00764-021-00134-4