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Spectroscopic studies on the drug–drug interaction: the influence of fluoroquinolones on the affinity of tigecycline to human serum albumin and identification of the binding site

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Abstract

Concomitant use of two or more drugs in therapy is becoming a more frequent phenomenon and clinically relevant drug–drug interactions at the level of binding to human serum albumin (HSA) are more often. The influence of fluoroquinolones–sparfloxacin, levofloxacin, and ciprofloxacin, on the interaction between tigecycline and HSA, was investigated in vitro by means of fluorescence and absorption spectroscopy. The results of UV–Vis and fluorescence spectroscopy showed that the fluorescence quenching of HSA was a result of the formation of new complexes through a static quenching process. The binding constants (Ka) and the number of binding sites (n) of all systems were calculated. The presence of sparfloxacin and ciprofloxacin increases and that of levofloxacin slightly decreases the binding constant of the HSA–tigecycline system. Competitive binding studies in the presence of site-specific markers showed that tigecycline was not significantly displaced by ibuprofen, but warfarin showed a significant displacement of tigecycline. These results suggest that the competitive binding of tigecycline and warfarin to HSA exists. The results confirm that the binding site of tigecycline is mainly located in Sudlow’s site I (subdomain IIA) of HSA.

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Acknowledgements

The authors are grateful to the Ministry of Science and Technological Development of the Republic of Serbia for financial support (Grant Nos 172016 and 175007).

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Authors and Affiliations

  1. Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovića 69, 34000, Kragujevac, Serbia

    Stefan D. Stojanović & Ratomir M. Jelić

  2. Department of Medical, Natural, Mathematical and Biotechnical Sciences, Faculty of Hotel Management and Tourism in Vrnjačka Banja, University of Kragujevac, Vojvođanska 5a, 36210, Vrnjačka Banja, Serbia

    Jovan M. Nićiforović & Sandra M. Živanović

  3. Department of Analytical Chemistry, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221, Belgrade, Serbia

    Jadranka V. Odović

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  1. Stefan D. Stojanović
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  2. Jovan M. Nićiforović
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  3. Sandra M. Živanović
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  4. Jadranka V. Odović
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  5. Ratomir M. Jelić
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Correspondence to Stefan D. Stojanović.

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Stojanović, S.D., Nićiforović, J.M., Živanović, S.M. et al. Spectroscopic studies on the drug–drug interaction: the influence of fluoroquinolones on the affinity of tigecycline to human serum albumin and identification of the binding site. Monatsh Chem 151, 999–1007 (2020). https://doi.org/10.1007/s00706-020-02627-0

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