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The complete genome sequences of a negative single-stranded RNA virus and a double-stranded RNA virus coinfecting the entomopathogenic fungus Beauveria bassiana Vuillemin

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Abstract

Beauveria bassiana Vuillemin is an entomopathogenic fungus that has been developed as a biological insecticide. B. bassiana can be infected by single or multiple mycoviruses, most of which are double-stranded RNA (dsRNA) viruses, while infections with single-stranded RNA (ssRNA) viruses, especially negative single-stranded RNA (-ssRNA) viruses, have been observed less frequently. In the present study, we sequenced and analyzed the complete genomes of two new different mycoviruses coinfecting a single B. bassiana strain: a -ssRNA virus which we have named "Beauveria bassiana negative-strand RNA virus 1" (BbNSRV1), and a dsRNA virus, which we have named "Beauveria bassiana orthocurvulavirus 1" (BbOCuV1). The genome of BbNSRV1 consists of a single segment of negative-sense, single-stranded RNA with a length of 6169 nt, containing a single open reading frame (ORF) encoding a putative RNA-dependent RNA polymerase (RdRp) with 1949 aa (220.1 kDa). BLASTx analysis showed that the RdRp had the highest sequence similarity (59.79%) to that of Plasmopara viticola lesion associated mononegaambi virus 2, a member of the family Mymonaviridae. This is the first report of a -ssRNA mycovirus infecting B. bassiana. The genome of BbOCuV1 consists of two dsRNA segments, 2164 bp and 1765 bp in length, respectively, with dsRNA1 encoding a protein with conserved RdRp motifs and 70.75% sequence identity to the putative RdRp of the taxonomically unassigned mycovirus Fusarium graminearum virus 5 (FgV5), and the dsRNA2 encoding a putative coat protein with sequence identity 64.26% to the corresponding protein of the FgV5. Phylogenetic analysis indicated that BbOCuV1 belongs to a taxonomically unassigned group of dsRNA mycoviruses related to members of the families Curvulaviridae and Partitiviridae. Hence, it might be the member of a new family that remains to be named and formally recognized.

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Acknowledgments

This study was supported by Jilin Project for Outstanding Talents (Teams) for Innovation and Entrepreneurship in Science and Technology (grant number 20230508011RC).

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Author notes

  1. Mengnan Xu and Hongyu Liu contributed equally to this work.

Authors and Affiliations

  1. Jilin Normal University, Si**, 136000, China

    Mengnan Xu, Yizhuo Lu, Zhengkun Zhang & Jianfeng Liu

  2. Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Jilin Key Laboratory of Agricultural Microbiology, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of agriculture and rural affairs, Changchun, 130033, China

    Mengnan Xu, Hongyu Liu, Xue Jia, **aowei Zou, Yizhuo Lu, Li Sui, Qiyun Li & Zhengkun Zhang

  3. Jilin Agricultural University, Changchun, 130118, China

    Hongyu Liu, Xue Jia, Qiyun Li & Zhengkun Zhang

  4. Jilin Agricultural Science and Technology University, Jilin, 132101, China

    Qiyun Li

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  1. Mengnan Xu
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  2. Hongyu Liu
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Correspondence to Zhengkun Zhang or Jianfeng Liu.

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Xu, M., Liu, H., Jia, X. et al. The complete genome sequences of a negative single-stranded RNA virus and a double-stranded RNA virus coinfecting the entomopathogenic fungus Beauveria bassiana Vuillemin. Arch Virol 169, 42 (2024). https://doi.org/10.1007/s00705-024-05985-w

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