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Biological and molecular characterization of bean bushy stunt virus, a novel bipartite begomovirus infecting common bean in northwestern Argentina

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Abstract

Common bean plants (Phaseolus vulgaris L.) showing different virus-like symptoms were collected in northwestern Argentina. Dot-blot hybridization tests showed that the begomoviruses bean golden mosaic virus and tomato yellow vein streak virus were the most prevalent, but they also revealed the presence of unknown begomoviruses. The complete genome sequence of one of these unknown begomoviruses was determined. Sequence analysis showed that the virus is a typical New World begomovirus, for which the name "bean bushy stunt virus" (BBSV) is proposed. Biological assays based on biolistic inoculations showed that BBSV induced leaf roll and stunting symptoms similar to those observed in the field-collected common bean sample.

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Acknowledgements

This work was supported by the Argentine Government, through the Instituto Nacional de Tecnología Agropecuaria (INTA) and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Pablo Gastón Reyna has a CONICET doctoral scholarship. The authors thank researchers Daniel Agustín Maidana, Juan Facundo Alamo, and Ceferino René Flores for field sampling.

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Authors and Affiliations

  1. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Unidad de Fitopatología y Modelización Agrícola (UFYMA), Avenida 11 de Septiembre 4755, X5014MGO, Córdoba, Argentina

    Pablo Gastón Reyna, Nicolás Bejerman, Irma Graciela Laguna & Patricia Rodríguez Pardina

  2. Instituto Nacional de Tecnología Agropecuaria (INTA), Centro de Investigaciones Agropecuarias (CIAP), Instituto de Patología Vegetal (IPAVE), Avenida 11 de Septiembre 4755, X5014MGO, Córdoba, Argentina

    Pablo Gastón Reyna, Nicolás Bejerman & Patricia Rodríguez Pardina

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  1. Pablo Gastón Reyna
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  3. Irma Graciela Laguna
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  4. Patricia Rodríguez Pardina
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Correspondence to Pablo Gastón Reyna.

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Supplementary file1 (DOC 100 KB) Supplementary Table 1 Begomoviruses used for pairwise sequence comparisons and phylogenetic and recombination analysis

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Reyna, P.G., Bejerman, N., Laguna, I.G. et al. Biological and molecular characterization of bean bushy stunt virus, a novel bipartite begomovirus infecting common bean in northwestern Argentina. Arch Virol 166, 1409–1414 (2021). https://doi.org/10.1007/s00705-021-05002-4

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