Abstract
Menghai flavivirus (MFV) was isolated from Aedes albopictus in Menghai county of Yunnan Province, China, during an arboviruses screening program in August 2010. Whole genome sequencing of MFV was performed using an Ion PGM™ Sequencer. The complete genome of MFV was 10897 nucleotides in length and encoded a polyprotein and fairly interesting flavivirus orf (FIFO). The polyprotein contained three flavivirus structural proteins (C, prM/M and E) and seven nonstructural proteins. Nucleotide BLAST analysis revealed that the MFV genome showed highest similarity to **shuangbanna Aedes flavivirus, a novel insect-specific flavivirus recently isolated from the same area. These species shared a query cover of 99%, but only 71% identity, while FIFO showed no similarity with any of the published sequences. Genomic and phylogenetic analyses suggested that MFV was a novel species of the genus Flavivirus. Our findings enrich our understanding of the genetics and prevalence of the family Flaviviridae.
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This research was supported by grants from the National Natural Science Foundation of China (No. 81273138 and No. 81572045), the China Mega-Project on Infectious Disease Prevention (No. 2013ZX10004-605, No. 2013ZX10004-607, No. 2013ZX10004-217 and No. 2011ZX10004-001) and the National Hi-Tech Research and Development (863) Program of China (No. 2014AA021402, No. 2012AA022-003 and No. 2015AA020108).
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X. Zhang and X. Guo contributed equally to this work.
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Zhang, X., Guo, X., Fan, H. et al. Complete genome sequence of Menghai flavivirus, a novel insect-specific flavivirus from China. Arch Virol 162, 1435–1439 (2017). https://doi.org/10.1007/s00705-017-3232-5
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DOI: https://doi.org/10.1007/s00705-017-3232-5