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Complete genomic sequence of barley (Hordeum vulgare) endornavirus (HvEV) determined by next-generation sequencing

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Abstract

Endornaviruses are unusual plant-, fungus- and oomycete-infecting viruses with a large, ca 14- to 17-kb linear double-stranded RNA (dsRNA) genome and a persistent lifestyle. The complete genome sequence of an endornavirus from the barley (Hordeum vulgare) Nerz variety was determined from paired Illumina MySeq reads derived from purified dsRNAs. The genome is 14,243 nt long, with 5’ and 3’ non-coding regions of 207 and 47 nt, respectively. It encodes a single large protein of 4663 amino acids that carries conserved motifs for a methyltransferase, a helicase and an RNA-dependent RNA polymerase. The sequence of Hordeum vulgare endornavirus (HvEV) carries all the hallmarks of a typical member of the genus Endornavirus, with the exception of an UDP-glycosyltransferase motif observed in many, but not all, endornaviral genomes.

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Acknowledgments

This work was supported by the CASDAR “MOSA-Hordeum” project (N°C-2013-08) financed by the French Ministère de l’Agriculture, de l’Agroalimentaire et de la Forêt (MAAF).

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Authors and Affiliations

  1. INRA, UMR 1332 Biologie du Fruit et Pathologie, CS 20032, 33882, Villenave d’Ornon Cedex, France

    Thierry Candresse, Armelle Marais, Chantal Faure & Sébastien Theil

  2. Université de Bordeaux, UMR 1332 Biologie du Fruit et Pathologie, CS 20032, 33882, Villenave d’Ornon Cedex, France

    Thierry Candresse, Armelle Marais, Chantal Faure & Sébastien Theil

  3. Dipartimento di Agraria, Università degli Studi di Napoli “Federico II”, 80055, Portici, Italy

    Roberto Sorrentino

  4. GEVES, 25 rue Georges Morel, CS 90024, 49071, Beaucouzé Cedex, France

    Valérie Cadot, Mathieu Rolland & Julie Villemot

  5. Institute for Epidemiology and Pathogen Diagnostics, Julius Kuhn-Institute, Federal Research Institute for Cultivated Plants, Erwin-Baur-Straße 27, 06484, Quedlinburg, Germany

    Frank Rabenstein

Authors
  1. Thierry Candresse
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  2. Armelle Marais
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  3. Roberto Sorrentino
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  4. Chantal Faure
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  5. Sébastien Theil
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  6. Valérie Cadot
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  7. Mathieu Rolland
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  8. Julie Villemot
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  9. Frank Rabenstein
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Corresponding author

Correspondence to Thierry Candresse.

Additional information

The nucleotide sequence reported in this work has been deposited in the GenBank database under accession number KT721705.

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Candresse, T., Marais, A., Sorrentino, R. et al. Complete genomic sequence of barley (Hordeum vulgare) endornavirus (HvEV) determined by next-generation sequencing. Arch Virol 161, 741–743 (2016). https://doi.org/10.1007/s00705-015-2709-3

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  • DOI: https://doi.org/10.1007/s00705-015-2709-3

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