SpringerLink
Log in

Identification and characterization of novel hepatitis B virus subgenotype C10 in Nusa Tenggara, Indonesia

  • Original Article
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Six novel subgenotypes (B7, B8, C6, C8, C9, and D6) within three hepatitis B virus (HBV) genotypes (B–D) were recently identified in Indonesia. To further characterize HBV in this country, 18 HBV-viremic samples obtained from blood donors in Nusa Tenggara, Indonesia, were subjected to phylogenetic analysis of an 1.6-kb partial or full-length sequence. Thirteen HBV isolates were classified into genotype B with four distinct subgenotypes [B3 (n = 2), B5 (n = 1), B7 (n = 4), and B8 (n = 6)], followed by 4 HBV isolates of genotype C (HBV/C); the remaining one isolate was of D (D1). As for the four HBV/C isolates, one isolate segregated into subgenotype C1, and two into C2. The remaining HBV/C isolate [C0901177(NT3)] differed from reported HBV/C isolates (C1–C9) by 4.6–7.7% over the entire genome and did not show evidence of recombination with any of the known HBV genotypes/subgenotypes, justifying its conclusive assignment into a novel subgenotype (C10) within genotype C.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Germany)

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Arauz-Ruiz P, Norder H, Robertson BH, Magnius LO (2002) Genotype H: a new Amerindian genotype of hepatitis B virus revealed in Central America. J Gen Virol 83:2059–2073

    CAS  PubMed  Google Scholar 

  2. Cavinta L, Sun J, May A, Yin J, von Meltzer M, Radtke M, Barzaga NG, Cao G, Schaefer S (2009) A new isolate of hepatitis B virus from the Philippines possibly representing a new subgenotype C6. J Med Virol 81:983–987

    Article  CAS  PubMed  Google Scholar 

  3. Cavinta L, Cao G, Schaefer S (2009) Description of a new hepatitis B virus C6 subgenotype found in the Papua province of Indonesia and suggested renaming of a tentative C6 subgenotype found in the Philippines as subgenotype C7. J Clin Microbiol 47:3068–3069

    Article  PubMed  Google Scholar 

  4. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  5. Huy TTT, Ngoc TT, Abe K (2008) New complex recombinant genotype of hepatitis B virus identified in Vietnam. J Virol 82:5657–5663

    Article  CAS  Google Scholar 

  6. Ina Y (1994) ODEN: a program package for molecular evolutionary analysis and database search of DNA and amino acid sequences. Comput Appl Biosci 10:11–12

    CAS  PubMed  Google Scholar 

  7. Khan M, Dong JJ, Acharya SK, Dhagwahdorj Y, Abbas Z, Jafri SM W, Mulyono DH, Tozun N, Sarin SK (2004) Hepatology issues in Asia: perspectives from regional leaders. J Gastroenterol Hepatol 19:S419–S430

    Article  Google Scholar 

  8. Kramvis A, Kew M, François G (2005) Hepatitis B virus genotypes. Vaccine 23:2409–2423

    Article  CAS  PubMed  Google Scholar 

  9. Kramvis A, Arakawa K, Yu MC, Nogueira R, Stram DO, Kew MC (2008) Relationship of serological subtype, basic core promoter and precore mutations to genotypes/subgenotypes of hepatitis B virus. J Med Virol 80:27–46

    Article  CAS  PubMed  Google Scholar 

  10. Lee WM (1997) Hepatitis B virus infection. N Engl J Med 337:1733–1745

    Article  CAS  PubMed  Google Scholar 

  11. Lole KS, Bollinger RC, Paranjape RS, Gadkari D, Kulkarni SS, Novak NG, Ingersoll R, Sheppard HW, Ray SC (1999) Full-length human immunodeficiency virus type 1 genomes from subtype C-infected seroconverters in India, with evidence of intersubtype recombination. J Virol 73:152–160

    CAS  PubMed  Google Scholar 

  12. Lusida MI, Nugrahaputra VE, Soetjipto, Handajani R, Nagano-Fujii M, Sasayama T, Utsumi T, Hotta H (2008) Novel subgenotypes of hepatitis B virus genotypes C and D in Papua, Indonesia. J Clin Microbiol 46:2160–2166

    Article  CAS  PubMed  Google Scholar 

  13. Miyakawa Y, Mizokami M (2003) Classifying hepatitis B virus genotypes. Intervirology 46:329–338

    Article  CAS  PubMed  Google Scholar 

  14. Mulyanto, Surayah K, Depamede SN, Lestarini IA, Budianto W, Hafiludin, Umi K, Tsuda F, Takahashi M, Okamoto H (2008) Hepatitis B virus infection in Eastern part of Indonesia. Abstract Book of the Second China–Indonesia Joint International Symposium on Hepatobiliary Medicine and Surgery, Chongqing, China

  15. Mulyanto, Depamede SN, Surayah K, Tsuda F, Ichiyama K, Takahashi M, Okamoto H (2009) A nationwide molecular epidemiological study on hepatitis B virus in Indonesia: identification of two novel subgenotypes, B8 and C7. Arch Virol 154:1047–1059

    Article  CAS  PubMed  Google Scholar 

  16. Norder H, Couroucé AM, Magnius LO (1994) Complete genomes, phylogenetic relatedness, and structural proteins of six strains of the hepatitis B virus, four of which represent two new genotypes. Virology 198:489–503

    Article  CAS  PubMed  Google Scholar 

  17. Norder H, Couroucé AM, Coursaget P, Echevarria JM, Lee SD, Mushahwar IK, Robertson BH, Locarnini S, Magnius LO (2004) Genetic diversity of hepatitis B virus strains derived worldwide: genotypes, subgenotypes, and HBsAg subtypes. Intervirology 47:289–309

    Article  PubMed  Google Scholar 

  18. Nurainy N, Muljono DH, Sudoyo H, Marzuki S (2008) Genetic study of hepatitis B virus in Indonesia reveals a new subgenotype of genotype B in east Nusa Tenggara. Arch Virol 153:1057–1065

    Article  CAS  PubMed  Google Scholar 

  19. Okamoto H, Tsuda F, Sakugawa H, Sastrosoewignjo RI, Imai M, Miyakawa Y, Mayumi M (1988) Ty** hepatitis B virus by homology in nucleotide sequence: comparison of surface antigen subtypes. J Gen Virol 69:2575–2583

    Article  CAS  PubMed  Google Scholar 

  20. Olinger CM, Jutavijittum P, Hübschen JM, Yousukh A, Samountry B, Thammavong T, Toriyama K, Muller CP (2008) Possible new hepatitis B virus genotype, southeast Asia. Emerg Infect Dis 14:1777–1780

    Article  PubMed  Google Scholar 

  21. Page RDM (1996) TREEVIEW: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358

    CAS  PubMed  Google Scholar 

  22. Pourkarim MR, Lemey P, Amini-Bavil-Olyaee S, Maes P, Van Ranst M (2010) Novel hepatitis B virus subgenotype A6 in African-Belgian patients. J Clin Virol 47:93–96

    Article  CAS  PubMed  Google Scholar 

  23. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  24. Sakamoto S, Tanaka Y, Orito E, Co J, Clavio J, Ito K, Ozasa A, Quino A, Ueda R, Sollano J, Mizokami M (2006) Novel subtypes (subgenotypes) of hepatitis B virus genotypes B and C among chronic liver disease patients in the Philippines. J Gen Virol 87:1873–1882

    Article  CAS  PubMed  Google Scholar 

  25. Stuyver L, De Gendt S, Van Geyt C, Zoulim F, Fried M, Schinazi RF, Rossau R (2000) A new genotype of hepatitis B virus: complete genome and phylogenetic relatedness. J Gen Virol 81:67–74

    CAS  PubMed  Google Scholar 

  26. Takahashi M, Nishizawa T, Gotanda Y, Tsuda F, Komatsu F, Kawabata T, Hasegawa K, Altankhuu M, Chimedregzen U, Narantuya L, Hoshino H, Hino K, Kagawa Y, Okamoto H (2004) High prevalence of antibodies to hepatitis A and E viruses and viremia of hepatitis B, C, and D viruses among apparently healthy populations in Mongolia. Clin Diagn Lab Immunol 11:392–398

    PubMed  Google Scholar 

  27. Tatematsu K, Tanaka Y, Kurbanov F, Sugauchi F, Mano S, Maeshiro T, Nakayoshi T, Wakuta M, Miyakawa Y, Mizokami M (2009) A genetic variant of hepatitis B virus divergent from known human and ape genotypes isolated from a Japanese patient and provisionally assigned to new genotype J. J Virol 83:10538–10547

    Article  CAS  PubMed  Google Scholar 

  28. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  CAS  PubMed  Google Scholar 

  29. Tiollais P, Charnay P, Vyas GN (1981) Biology of hepatitis B virus. Science 213:406–411

    Article  CAS  PubMed  Google Scholar 

  30. Utsumi T, Lusida MI, Yano Y, Nugrahaputra VE, Amin M, Juniastuti Soetjipto, Hayashi Y, Hotta H (2009) Complete genome sequence and phylogenetic relatedness of hepatitis B virus isolates in Papua, Indonesia. J Clin Microbiol 47:1842–1847

    Article  CAS  PubMed  Google Scholar 

  31. Wang Z, Huang Y, Wen S, Zhou B, Hou J (2007) Hepatitis B virus genotypes and subgenotypes in China. Hepatol Res 37(s1):S36–S41

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This study was supported in part by a grant from the Ministry of Education, Culture, Sports, Science and Technology of Japan. The authors are grateful to Dr. Teron of Kupang Blood Center, who helped us to collect blood samples, for his invaluable contributions.

Author information

Authors and Affiliations

  1. Immunobiology Laboratory, Faculty of Medicine, University of Mataram, Mataram, Indonesia

    Mulyanto & Sulaiman Ngongu Depamede

  2. West Nusa Tenggara Hepatitis Laboratory, Mataram, Indonesia

    Mulyanto & Kiely Surayah

  3. Biomedical Laboratory, Faculty of Medicine, University of Nusa Cendana, Kupang, Indonesia

    Andreas Andhry Heru Tjahyono

  4. Division of Virology, Department of Infection and Immunity, Jichi Medical University School of Medicine, 3311-1 Yakushiji, Shimotsuke, Tochigi, 329-0498, Japan

    Jirintai, Shigeo Nagashima, Masaharu Takahashi & Hiroaki Okamoto

Authors
  1. Mulyanto
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sulaiman Ngongu Depamede
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kiely Surayah
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Andreas Andhry Heru Tjahyono
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jirintai
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Shigeo Nagashima
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Masaharu Takahashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Hiroaki Okamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hiroaki Okamoto.

Additional information

The nucleotide sequence data reported in this study have been assigned DDBJ/EMBL/GenBank accession numbers AB540582-AB540585 for 4 full-length HBV genomes and AB540586-AB540599 for 14 1.6-kb partial HBV sequences.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mulyanto, Depamede, S.N., Surayah, K. et al. Identification and characterization of novel hepatitis B virus subgenotype C10 in Nusa Tenggara, Indonesia. Arch Virol 155, 705–715 (2010). https://doi.org/10.1007/s00705-010-0628-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-010-0628-x

Keywords

Search

Navigation