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Relationship between U83 gene variation in human herpesvirus 6 and secretion of the U83 gene product

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Abstract

The betaherpesvirus human herpesvirus 6 (HHV-6) has two variants. The U83 gene product of strain HST is a chemoattractant for monocytes. Here, we describe U83 gene variations that accumulated in variants A and B. A gene-variation hot spot was examined in 36 different strains and one donor DNA sample. U83 gene variations accumulated in variant A and in reactivated variant B after transplantation. None of the variant-A viruses encoded the signal peptide found in the B variant. U83 gene sequencing suggested that the variant A and B groups were separate, and that the variant B viruses could be further divided into the HST-Z29 type and another type with a shorter signal peptide. In a eukaryotic expression system, the HST-Z29 type of U83 gene product was secreted into the medium, a frame-shifted HST-Z29 type was partially secreted, and the variant-A type and a first-methionine knockout of the HST-Z29 type were not secreted.

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Acknowledgments

We thank the clinical staff, especially Dr. K. Tanaka-Taya and Dr. Y. Yamagishi, of the Department of Pediatrics, Graduate School of Medicine, Osaka University, for their help in collecting the ES virus samples and the DNA sample of Japanese HHV-6A.

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Authors and Affiliations

  1. Department of Infectious Disease Control, Graduate School of Medicine G5, Osaka University, 2-2 Yamada-Oka Suita, Osaka, 565-0871, Japan

    R. Sjahril, Yuji Isegawa, K. Nakano & N. Sugimoto

  2. Center for Medical Research and Education, C-10, Graduate School of Medicine, Osaka University Suita, Osaka, 565-0871, Japan

    T. Tanaka

  3. Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, 470-1192, Japan

    T. Yoshikawa & Y. Asano

  4. Gene Engineering Laboratory, Genomics Program, Nagahamabio Institute of Bio-Science and Technology, Nagahama, Shiga, 526-0829, Japan

    A. Ohshima

  5. National Institute of Biomedical Innovation, Ibaraki, Osaka, 565-0085, Japan

    K. Yamanishi

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  1. R. Sjahril
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  2. Yuji Isegawa
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  3. T. Tanaka
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  4. K. Nakano
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  8. K. Yamanishi
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Correspondence to Yuji Isegawa.

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Sjahril, R., Isegawa, Y., Tanaka, T. et al. Relationship between U83 gene variation in human herpesvirus 6 and secretion of the U83 gene product. Arch Virol 154, 273–283 (2009). https://doi.org/10.1007/s00705-008-0307-3

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  • DOI: https://doi.org/10.1007/s00705-008-0307-3

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