Abstract
The study considers the phylogenetic affinities and taxonomical placement of the previously unknown hepatic from Hengduan Mts. in Yunnan Province of China. This area is known to house a vast assemblage of peculiar and taxonomically secluded taxa of hepatics, serving as kind of storehouse of missing links in the evolution. The newly recorded taxon combines lophozioid appearance with prominent tooth in the antical side of the leaf—the feature previously observed in Cephaloziineae very rarely and in bracts only. Molecular phylogenetic reconstruction, inferred from the nuclear ITS region and the chloroplast rps4 gene, trnL-F region and trnG-intron resolved Konstantinovia gen. nov. as the closest relative of highly phylogenetically isolated monospecific genus Obtusifolium. Their joint clade is sister to the Anastrophyllaceae clade in the tree inferred from combined ITS and trnL sequences, while topology inferred from combined trnG and rps4 sequences fails to resolve its affinity within the Scapaniaceae s.l. clade. Therefore, the new family Obtusifoliaceae is introduced to accommodate these genera, and the familial classification of Cephaloziineae is discussed.
Similar content being viewed by others
Availability of data and materials (data transparency)
The material was legally collected by the permission from Botanical Garden in Kunming and parts of collected material was transferred abroad (to Russian Federation) with corresponding permissions. The studied material is now available in VBGI, KUN and MW.
References
Bakalin VA (2005) Monograficheskaia obrabotka roda Lophozia (Dumort.) Dumort. s. str. Nauka, Moscow (in Russian)
Bakalin VA (2010) Distribution of bryophytes in the Russian Far East. Part. I. Hepatics. Izd-vo DVFU, Vladivostok
Bakalin V, Vilnet A (2014) Two new species of the liverwort genus Hygrobiella Spruce (Marchantiophyta) described from the North Pacific based on integrative taxonomy. Pl Syst Evol 300:2277–2291
Bakalin V, Vilnet A (2018) A review of the genus Diplophyllum (Marchantiophyta) in North and East Asia with the description of a new species (D. sibiricum) based on integrative taxonomy. Pl Syst Evol 304:1269–1287. https://doi.org/10.1007/s00606-018-1547-7
Bakalin VA, Fedosov VE, Fedorova AV, Nguyen VS (2019a) Integrative taxonomic revision of Marsupella (Gymnomitriaceae, Hepaticae) reveals neglected diversity in Pacific Asia. Cryptog Bryol 40:59–85. https://doi.org/10.5252/cryptogamie-bryologie2019v40a7
Bakalin V, Vilnet A, Ma WZ, Klimova K (2019b) The differentiation and speciation of Scapania javanica and S. undulata complexes in the Eastern Sino-Himalayas and perimeters for Scapania Sect. Stephania (Scapaniaceae, Hepaticae). Phytotaxa 400:123–144 https://doi.org/10.11646/phytotaxa.400.3.2
Bakalin V, Vilnet A, Nguyen VS (2020a) Vietnamiella epiphytica – a new genus and species of Anastrophyllaceae (Hepaticae). Bryologist 123:48–63
Bakalin VA, Fedosov VE, Maltseva YD, Milyutina IA, Klimova KG, Nguyen HM, Troitsky AV (2020b) Overview of Schistochilopsis (Hepaticae) in Pacific Asia with the Description Protochilopsis gen. nov. Plants 9:850
Bisang I (1991) Biosystematische Studien an Lophozia subg. Schistochilopsis (Hepaticae). Bryophyt Biblioth 43:1–187
Chen P-C (1955) Bryophyta nova sinica. Feddes Repert 58:23–52
Chen YS, Deng T, Zhou Z, Sun H (2018) Is the east Asian flora ancient or not? Natl Sci Rev 5:142–154. https://doi.org/10.1093/nsr/nwx156
de Roo RT, Hedderson TA, Söderström L (2007) Molecular insights into the phylogeny of the leafy liverwort family Lophoziaceae Cavers. Taxon 56:301–314
Fedosov VE, Fedorova AV, Fedosov AE, Ignatov MS (2016) Phylogenetic inference and peristome evolution in haplolepideous mosses, focusing on Pseudoditrichaceae and Ditrichaceae s. l. Bot J Linn Soc 181:139–155
Gao C, Chang K-C, Cao T (1981) Taxa nova bryophytarum Tibeticarum. Acta Bot Yunnan 3:389–399
Gao C, Cao T (eds) (2000) Flora Yunnanica Tomus 17 (Bryophyta: Hepaticae, Anthocerotae). Science Press, Bei**g
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 41:95–98
Hodgetts NG, Söderström L, Blockeel TL, Caspari S, Ignatov MS, Konstantinova NA, Lockhart N, Papp B, Schröck C, Sim-Sim M, Bell D, Bell NE, Blom HH, Bruggeman-Nannenga MM, Brugués M, Enroth J, Flatberg KI, Garilleti R, Hedenäs L, Holyoak DT, Hugonnot V, Kariyawasam I, Köckinger H, Kučera J, Lara F, Porley RD (2020) An annotated checklist of bryophytes of Europe, Macaronesia and Cyprus. J Bryol 42:1–116. https://doi.org/10.1080/03736687.2019.1694329
Katoh K, Standley DN (2013) MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability. Molec Biol Evol 30:772–780
Konstantinova NA, Bakalin VA, Andrejeva EN, Bezgodov AG, Borovichev EA, Dulin MV, Mamontov YM (2009) Checklist of liverworts (Marchantiophyta) of Russia. Arctoa 18:1–64
Kowal J, Pressel S, Duckett JG, Bidartondo MI, Field KJ (2018) From rhizoids to roots? Experimental evidence of mutualism between liverworts and ascomycete fungi. Ann Bot (Oxford) 121:221–227. https://doi.org/10.1093/aob/mcx126
Laenen B, Shaw B, Schneider H, Goffinet B, Paradis E, De Samore A, Heinrichs J, Villarreal JC, Gradstein SR, McDaniel SF, Long DG, Forrest LL, Hollingsworth ML, Crandall-Stotler B, Davis EC, Engel J, Von Konrat M, Cooper ED, Patiňo J, Cox CJ, Vanderpoorten A, Shaw AJ (2014) Extant diversity of bryophytes emerged from successive post-Mesozoic diversification bursts. Nat Commun 5:1–6. https://doi.org/10.1038/ncomms6134
Lanfear R, Calcott B, Ho SYW, Guindon S (2012) PartitionFinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molec Biol Evol 29:1695–1701
Masuzaki H, Tsubota H, Shimamura M, Yamaguchi T, Deguchi H (2010) A taxonomic revision of the genus Apometzgeria (Metzgeriaceae, Marchantiophyta). Hikobia 15:427–452
Miller MA, W. Pfeiffer W, Schwartz T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceedings of the Gateway Computing Environments Workshop (GCE), 14 Nov. 2010, New Orleans, LA, pp 1–8
Mitten W (1860) Hepaticae Indiae Orientalis. J Proc Linn Soc Bot 5:89–128
Müller K (2005) SeqState. Appl Bioinf 4:65–69
Myers N, Mittermeier RA, Mittermeier CG, da Fonse GAB, Kent J (2000) Biodiversity hotspots for conservation priorities. Nature 403:853–858
Patzak SDF, Schaefer-Verwimp A, Váňa J, Renner MAM, Peralta DF, Heinrichs J (2016) Chonecoleaceae (Lophocoleineae) is a synonym of Cephaloziellaceae (Cephaloziineae) and Rivulariella (Jungermanniineae) belongs to Scapaniaceae s.l. (Cephaloziineae). Phytotaxa 267:91. https://doi.org/10.11646/phytotaxa.267.2.1
Potemkin AD (2005) On systematic position and taxonomic status of the genera Schistochilopsis (Kitag.) Konstantinova and Obtusifolium, S.W. Arnell (Lophoziaceae, Hepaticae). Nov Sist Nizsh Rast 38:362–365 (in Russian)
Pressel S, Bidartondo MI, Ligrone R, Duckett JG (2010) Fungal symbioses in bryophytes: New insights in the Twenty First Century. Phytotaxa 9:238–253
Prothero D (2007) Evolution: What the Fossils Say and Why It Matters. Columbia University Press, New York
Rambaut A, Drummond AJ (2007) BEAST. Computer program and documentation. Distributed by the author. Available at: http://beast.bio.ed.ac.uk/Tracer
Ronquist F, Teslenko M, Van Der Mark P, Ayres DL, Darling S, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol 61:539–542
Schuster RM (1966) The Hepaticae and Anthocerotae of North America. I. Columbia University Press, New York
Schuster RM (1969) The Hepaticae and Anthocerotae of North America. Columbia University Press, New York, II
Schuster RM (1970) Studies on Hepaticae. XVIII. The family Jungermanniaceae, s. lat.: a reclassification. Trans Brit Bryol Soc 6:86–107
Shaw B, Crandall-Stotler B, Váňa J, Stotler RE, von Konrat M, Engel JJ, Davis CE, Long DG, Sova P, Shaw AJ (2015) Phylogenetic relationships and morphological evolution in a major clade of leafy liverworts (phylum Marchantiophyta, order Jungermanniales): suborder Jungermanniineae. Syst Bot 40:27–45. https://doi.org/10.1600/036364415X686314
Simmons MP, Ochoterena H (2000) Gaps as characters in sequence-based phylogenetic analyses. Syst Biol 49:369–381
Söderström L, de Roo RT, Hedderson TAJ (2010) Taxonomic novelties resulting from recent reclassification of the Lophoziaceae/Scapaniaceae clade. Phytotaxa 3:47–53. https://doi.org/10.11646/phytotaxa.3.1.7
Söderström L, Hagborg A, von Konrat M, Bartholomew-Began S, Bell D, Briscoe L, Brown E, Cargill DC, Costa DP, Crandall-Stotler BJ, Cooper ED, Dauphin G, Engel JJ, Feldberg K, Glenny D, Gradstein SR, He X, Heinrichs J, Hentschel J, Ilkiu-Borges AL, Katagiri T, Konstantinova NA, Larrain J, Long DG, Nebel M, Pócs T, Felisa Puche F, Reiner-Drehwald E, Renner MAM, Sass-Gyarmati A, Schäfer-Verwimp A, Moragues JGS, Stotler RE, Sukkharak P, Thiers BM, Uribe J, Váňa J, Villarreal JC, Wigginton M, Zhang L, Zhu R-L (2016) World checklist of hornworts and liverworts. PhytoKeys 59:1–828. https://doi.org/10.3897/phytokeys.59.6261
Struck TH, Feder JL, Bendiksby M, Birkeland S, Cerca J, Gusarov VI, Kistenich S, Larsson KH, Liow LH, Nowak MD, Stedje B, Bachmann L, Dimitrov D (2018) Finding Evolutionary Processes Hidden in Cryptic Species. Trends Ecol Evol 33:153–163. https://doi.org/10.1016/j.tree.2017.11.007
Sun H, Zhang JW, Deng T (2017) Origins and evolution of plant diversity in the Hengduan Mountains. China Pl Diversity 39:161–166
Tan BC, Iwatsuki Z (1996) Hot spots of mosses in East Asia. Hot spots of mosses in East Asia. Anales Inst Biol Univ Nac Auton Mexico, Bot 67:159–167
Váňa J, Long DG (2008) Hamatostrepta concinna gen. et sp. nov. (Jungermanniopsida, Scapaniaceae), a new Asiatic leafy liverwort from the Sino-Burmese border. Fieldiana, Bot 47:133–138. https://doi.org/10.3158/0015-0746-47.1.133
Váňa J, Söderström L, Hagborg A, von Konrat MJ (2013) Notes on Early Land Plants Today. 40. Notes on Cephaloziellaceae (Marchantiophyta). Phytotaxa 112:1–6. https://doi.org/10.11646/phytotaxa.112.1.1
Vilnet AA, Konstantinova NA, Troitsky AV (2008) Phylogeny and systematics of the genus Lophozia s. str. (Dumort.) Dumort. (Hepaticae) and related taxa from nuclear ITS1–2 and chloroplast trnL-F sequences. Molec Phylogen Evol 47:403–418
Vilnet AA, Konstantinova NA, Troitsky AV (2010) Molecular insight on phylogeny and systematics of the Lophoziaceae, Scapaniaceae, Gymnomitriaceae and Jungermanniaceae. Arctoa 19:31–50
Ward FK (1921) The Mekong-Salween Divide as a geographical barrier. Geogr J 58:49–56. https://doi.org/10.2307/1780720
Ward FK (1925) Sino-Himalaya. Nature 116:282–284. https://doi.org/10.1038/116282a0
Ward FK (1927) The Sino-Himalayan Flora. Proc Linn Soc London 139:67–74. https://doi.org/10.1111/j.1095-8312.1927.tb00074.x
Zhang LS (2012) Palaeogeography of China: the Formation of China’s Natural Environment. Science Press, Bei**g
Acknowledgements
The work was partially supported by the Russian Foundation for Basic Research (grant 20-04-00278 for VB). The work of VF was supported by MSU research project no. AAAA-A16-116021660039-1, the work of AF was partly supported by the institutional research project no. 118021490111–5. The line drawings were provided by Mr. Matvei Bakalin to whom authors are sincerely grateful.
Funding
Russian Foundation for Basic Research (grant 20-04-00278 for VB), Moscow State University (project no. AAAA-A16-116021660039-1 for VF), Main Botanical Garden institutional research project (no. 118021490111–5 for AF) and Botanical Garden-Institute research project (no. АААА-А20-120031990012-4 for VB).
Author information
Authors and Affiliations
Contributions
VB and VF contributed to general concept manuscript writing, VF and AF contributed to molecular analysis, VB and VF contributed to morphological implications, and VB and MWZ contributed to plant collecting.
Corresponding author
Ethics declarations
Conflict of interest
Authors declare no conflict of interest.
Ethics approval
Authors follow ethical standards required in the preparation of scientific papers.
Consent to participate
All authors are agree to work on preparation of this paper together.
Consent for publication
All authors agree to submit the manuscript for publication in its present form. (include appropriate statements).
Additional information
Handling editor: Karol Marhold.
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Below is the link to the electronic supplementary material.
Information on Electronic Supplementary Material
Information on Electronic Supplementary Material
Online resource 1. a Specimens and GenBank accession numbers used in reconstruction based on rps4 + trnG sequences (newly obtained sequences are provided in bold). b Specimens and GenBank accession numbers used in reconstruction based on ITS + trnLF sequences (newly obtained sequences are provided in bold). c Voucher data of the specimens originally studied for molecular phylogenetic reconstructions.
Online resource 2. The combined alignment ITS & trnL-F dataset.
Online resource 3. The combined alignment rps4 & trnG dataset.
Online resource 4. h ML tree inferred from rps4 & trnG dataset, indels are coded using simple indel coding technique. i ML tree inferred from rps4 & trnG dataset, indels are not considered. j ML tree inferred from ITS & trnL-F dataset, indels are coded using simple indel coding technique. k ML tree inferred from ITS & trnL-F dataset, indels are not considered. l ML tree inferred from ITS dataset, indels are not considered.
Online resource 5. Konstantinovia pulchra Bakalin & Fedosov: a the cushion in dry condition (holotype, VBGI); b plant habit, vital (holotype, VBGI); c plant habit, vital (holotype, VBGI); d plant habit, vital (holotype, VBGI); e gemmae (holotype, VBGI); f gemmae (holotype, VBGI); g midleaf cells showing oil bodies, vital (holotype, VBGI).
Rights and permissions
About this article
Cite this article
Bakalin, V.A., Fedosov, V.E., Fedorova, A.V. et al. Obtusifoliaceae, a new family of leafy liverworts to accommodate Konstantinovia, newly described from the Hengduan Mts. (South China) and Obtusifolium (Cephaloziineae, Marchantiophyta). Plant Syst Evol 307, 62 (2021). https://doi.org/10.1007/s00606-021-01779-8
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s00606-021-01779-8