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Rapid detection of melamine by DNA Walker mediated SERS sensing technique based on signal amplification function

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Abstract

A new method is proposed for detecting typical melamine dopants in food using surface-enhanced Raman scattering (SERS) biosensing technology. Melamine specific aptamer was used as the identification probe, and gold magnets (AuNPs@MNPs) and small gold nanoparticles (AuNPs@MBA) were used as the basis for Raman detection. The Raman signal of the detection system can directly detect melamine quantitatively. Under optimized conditions, the detection of melamine was carried out in the low concentration range of 0.001–500 mg/kg, the enhancement factor (EF) was 2.3 × 107, and the detection limit was 0.001 mg/kg. The method is sensitive and rapid, and can be used for the rapid detection of melamine in the field environment.

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Acknowledgments

I would like to express my heartfelt thanks to my tutor Zhixian Gao and teacher Shuyue Ren for their academic guidance, my Senior brother Ruipeng Chen for his writing help, and my classmates Jiaqi Lin, Han Cui and Rui Zhang for their summary.

Funding

The National Key Research and Development Program of China (2021YFA0910200) for funding this research project.

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Authors and Affiliations

  1. State Key Laboratory of Food Nutrition and Safety, Tian** University of Science and Technology, Tian**, 300457, China

    Yu**g Ma, Han Cui, Rui Zhang & Jun Liang

  2. Tian** Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tian** Institute of Environmental and Operational Medicine, Tian**, 300050, China

    Yu**g Ma, Han Cui, Ruipeng Chen, Rui Zhang, Jiaqi Lin, Shuyue Ren & Zhixian Gao

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  1. Yu**g Ma
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  2. Han Cui
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Contributions

Yu**g Ma: Conceptualization, Methodology, Validation, Writing—Original Draft, Writing—Review & Editing.

Han Cui: Resources, Formal analysis.

Ruipeng Chen: Formal analysis, Resources, Writing—Original Draft, Writing—Review & Editing.

Rui Zhang: Resources.

Jiaqi Lin: Resources, Formal analysis.

Shuyue Ren: Data Curation, Figure Processing, Investigation, Methodology, Formal analysis.

Jun Liang: Supervision, Project administration.

Zhixian Gao: Project administration, Funding acquisition.

Corresponding authors

Correspondence to Shuyue Ren, Jun Liang or Zhixian Gao.

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Ma, Y., Cui, H., Chen, R. et al. Rapid detection of melamine by DNA Walker mediated SERS sensing technique based on signal amplification function. Microchim Acta 191, 283 (2024). https://doi.org/10.1007/s00604-024-06336-x

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